The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
ATP-dependent activation of p21 WAF1/CIP1 -associated Cdk2 by Cdc6
Abstract
When cells progressing in mid-S phase are damaged with a base-modifying chemical, they arrest in S phase long after the CHK1 checkpoint signal fades out, partly because of p53-mediated long-lasting induction of the cyclin-dependent kinase inhibitor p21 WAF1/CIP1 . We have recently found that enforced expression of Cdc6, the assembler of prereplicative complexes, markedly advances recovery from the prolonged S-phase arrest and reactivation of Cdk2 despite the presence of a high level of induced p21. Here, we report that Cdc6 protein can activate p21-associated Cdk2 in an ATP-dependent manner in vitro . Consistently, Cdc6 mutated for ATPase or a putative cyclin binding motif is no longer able to activate the Cdk2 in vitro or promote reinitiation of S-phase progression and reactivation of Cdk2 in vivo . These results reveal the never anticipated function of Cdc6 and redefine its role in the control of S-phase progression in mammalian cells.
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“E7-expressing cells were shown to have upregulated cdc6 [143], which promotes cell cycle progression by activating cdk2 [144,145], and furthermore, cdc6 was shown to be important in the G1/S transition in E7-expressing cells under hypoxic conditions [146]”
From The Not-So-Good, the Bad and the Ugly: HPV E5, E6 and E7 Oncoproteins in the Orchestration of Carcinogenesis (2021) · cites this paper as [144]
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Cites
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p53 accumulates but is functionally impaired when DNA synthesis is blocked
Gottifredi, V., Shieh, S.-Y., Taya, Y., & Prives, C. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.98.3.1036 · Cited by 147
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Cited by
Basukala, O., & Banks, L. (2021) · Viruses · doi:10.3390/v13101892 · Cited by 137
Cdk2 is required for p53-independent G2/M checkpoint control
Chung, J. H., & Bunz, F. (2010) · PLoS Genetics · doi:10.1371/journal.pgen.1000863 · Cited by 105
Metadata
- Authors
- Kan, Q., Jinno, S., Yamamoto, H., Kobayashi, K., & Okayama, H.
- Affiliations
- Department of Biochemistry and Molecular Biology, Graduate School and Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2008-03-25
- Volume
- 105
- Issue
- 12
- Pages
- 4757-4762
- ISSN
- 0027-8424
- 1091-6490
- Cited by
- 19
- References
- 2
- Language
- en
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