The DOI matches its Crossref record; 59 of the 59 references it cites were found in a registry or an index.
A moderately thermophilic ammonia-oxidizing crenarchaeote from a hot spring
Open accessAbstract
The recent discovery of ammonia-oxidizing archaea (AOA) dramatically changed our perception of the diversity and evolutionary history of microbes involved in nitrification. In this study, a moderately thermophilic (46°C) ammonia-oxidizing enrichment culture, which had been seeded with biomass from a hot spring, was screened for ammonia oxidizers. Although gene sequences for crenarchaeotal 16S rRNA and two subunits of the ammonia monooxygenase ( amoA and amoB ) were detected via PCR, no hints for known ammonia-oxidizing bacteria were obtained. Comparative sequence analyses of these gene fragments demonstrated the presence of a single operational taxonomic unit and thus enabled the assignment of the amoA and amoB sequences to the respective 16S rRNA phylotype, which belongs to the widely distributed group I.1b (soil group) of the Crenarchaeota . Catalyzed reporter deposition (CARD)–FISH combined with microautoradiography (MAR) demonstrated metabolic activity of this archaeon in the presence of ammonium. This finding was corroborated by the detection of amoA gene transcripts in the enrichment. CARD-FISH/MAR showed that the moderately thermophilic AOA is highly active at 0.14 and 0.79 mM ammonium and is partially inhibited by a concentration of 3.08 mM. The enriched AOA, which is provisionally classified as “ Candidatus Nitrososphaera gargensis,” is the first described thermophilic ammonia oxidizer and the first member of the crenarchaeotal group I.1b for which ammonium oxidation has been verified on a cellular level. Its preference for thermophilic conditions reinvigorates the debate on the thermophilic ancestry of AOA.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Nucleic acid techniques in bacterial systematics
Stackebrandt, E. S., & Goodfellow, M. (1991) · Wiley eBooks · Cited by 9,460
ARB: a software environment for sequence data
Ludwig, W. (2004) · Nucleic Acids Research · doi:10.1093/nar/gkh293 · Cited by 6,702
Isolation of an autotrophic ammonia-oxidizing marine archaeon
Könneke, M., Bernhard, A. E., de la Torre, J. R., Walker, C. B., Waterbury, J. B., & Stahl, D. A. (2005) · Nature · doi:10.1038/nature03911 · Cited by 2,857
Archaea predominate among ammonia-oxidizing prokaryotes in soils
Leininger, S., Urich, T., Schloter, M., Schwark, L., Qi, J., Nicol, G. W., Prosser, J. I., Schuster, S. C., & Schleper, C. (2006) · Nature · doi:10.1038/nature04983 · Cited by 2,497
Ubiquity and diversity of ammonia-oxidizing archaea in water columns and sediments of the ocean
Francis, C. A., Roberts, K. J., Beman, J. M., Santoro, A. E., & Oakley, B. B. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0506625102 · Cited by 2,381
Molecular Microbial Ecology Manual
(1996) · Springer eBooks · doi:10.1007/978-94-009-0215-2 · Cited by 1,687
Missing lithotroph identified as new planctomycete
Strous, M., Fuerst, J. A., Kramer, E. H. M., Logemann, S., Muyzer, G., van de Pas-Schoonen, K. T., Webb, R., Kuenen, J. G., & Jetten, M. S. M. (1999) · Nature · doi:10.1038/22749 · Cited by 1,575
Archaeal nitrification in the ocean
Wuchter, C., Abbas, B., Coolen, M. J. L., Herfort, L., van Bleijswijk, J., Timmers, P., Strous, M., Teira, E., Herndl, G. J., Middelburg, J. J., et al. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0600756103 · Cited by 1,122
Purkhold, U., Pommerening-Röser, A., Juretschko, S., Schmid, M. C., Koops, H.-P., & Wagner, M. (2000) · Applied and Environmental Microbiology · doi:10.1128/aem.66.12.5368-5382.2000 · Cited by 1,102
Self-citationPernthaler, A., Pernthaler, J., & Amann, R. (2002) · Applied and Environmental Microbiology · doi:10.1128/aem.68.6.3094-3101.2002 · Cited by 1,065
Cited by
Nitrososphaera viennensis , an ammonia oxidizing archaeon from soil
Tourna, M., Stieglmeier, M., Spang, A., Könneke, M., Schintlmeister, A., Urich, T., Engel, M., Schloter, M., Wagner, M., Richter, A., et al. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1013488108 · Cited by 938
Self-citationPhysiology and Diversity of Ammonia-Oxidizing Archaea
Stahl, D. A., & de la Torre, J. R. (2012) · Annual Review of Microbiology · doi:10.1146/annurev-micro-092611-150128 · Cited by 593
Diversity, Physiology, and Niche Differentiation of Ammonia-Oxidizing Archaea
Hatzenpichler, R. (2012) · Applied and Environmental Microbiology · doi:10.1128/aem.01960-12 · Cited by 532
Self-citationArchaea and the Human Gut: New Beginning of an Old Story
Gaci, N., Borrel, G., Tottey, W., O’Toole, P. W., & Brugère, J.-F. (2014) · World Journal of Gastroenterology · doi:10.3748/wjg.v20.i43.16062 · Cited by 422
Gubry-Rangin, C., Nicol, G. W., & Prosser, J. I. (2010) · FEMS Microbiology Ecology · doi:10.1111/j.1574-6941.2010.00971.x · Cited by 419
Metadata
- Authors
- Hatzenpichler, R., Lebedeva, E. V., Spieck, E., Stoecker, K., Richter, A., Daims, H., & Wagner, M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2008-02-12
- Volume
- 105
- Issue
- 6
- Pages
- 2134-2139
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2130167404
- Cited by
- 674
- References
- 59
- FWCI
- 52.26
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: A moderately thermophilic ammonia-oxidizing crenarchaeote from a hot spring. https://app.cochat.ai/papers/10.1073/pnas.0708857105
- The paper
- Hatzenpichler, R., Lebedeva, E. V., Spieck, E., Stoecker, K., Richter, A., Daims, H., & Wagner, M. (2008). A moderately thermophilic ammonia-oxidizing crenarchaeote from a hot spring. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0708857105
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
