Cell proliferation at 122°C and isotopically heavy CH 4 production by a hyperthermophilic methanogen under high-pressure cultivation
Open accessAbstract
We have developed a technique for cultivation of chemolithoautotrophs under high hydrostatic pressures that is successfully applicable to various types of deep-sea chemolithoautotrophs, including methanogens. It is based on a glass-syringe-sealing liquid medium and gas mixture used in conjunction with a butyl rubber piston and a metallic needle stuck into butyl rubber. By using this technique, growth, survival, and methane production of a newly isolated, hyperthermophilic methanogen Methanopyrus kandleri strain 116 are characterized under high temperatures and hydrostatic pressures. Elevated hydrostatic pressures extend the temperature maximum for possible cell proliferation from 116°C at 0.4 MPa to 122°C at 20 MPa, providing the potential for growth even at 122°C under an in situ high pressure. In addition, piezophilic growth significantly affected stable carbon isotope fractionation of methanogenesis from CO 2 . Under conventional growth conditions, the isotope fractionation of methanogenesis by M. kandleri strain 116 was similar to values (−34‰ to−27‰) previously reported for other hydrogenotrophic methanogens. However, under high hydrostatic pressures, the isotope fractionation effect became much smaller (<−12‰), and the kinetic isotope effect at 122°C and 40 MPa was −9.4‰, which is one of the smallest effects ever reported. This observation will shed light on the sources and production mechanisms of deep-sea methane.
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High-pressure environments are often associated with high temperatures, such as in submarine hydrothermal vents or the deep continental subsurface.
“For example, high-pressure environments are often associated with high temperatures, such as in submarine hydrothermal vents or the deep continental subsurface (e.g., Takai et al., 2008), and low pH often causes an excess of heavy metals (e.g., Robbins et al., 2000).”
From The Astrobiology Primer v2.0 (2016) · cites this paper as Takai et al., 2008
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- Authors
- Takai, K., Nakamura, K., Toki, T., Tsunogai, U., Miyazaki, M., Miyazaki, J., Hirayama, H., Nakagawa, S., Nunoura, T., & Horikoshi, K.
- Affiliations
- Japan Agency for Marine-Earth Science and Technology (JP)
- University of the Ryukyus (JP)
- Hokkaido University (JP)
- *Subground Animalcule Retrieval Program and
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2008-08-05
- Volume
- 105
- Issue
- 31
- Pages
- 10949-10954
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC2490668
- OpenAlex
- W1983384197
- Cited by
- 848
- References
- 1
- Citations by year
- 2026: 53 · 2025: 49 · 2024: 57 · 2023: 54 · 2022: 48 · 2021: 66 · 2020: 63 · 2019: 55 · 2018: 62 · 2017: 48 · 2016: 44 · 2015: 32 · 2014: 46 · 2013: 48 · 2012: 36
- FWCI
- 20.69
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (18684027)
- Topics
- Physical Sciences › Environmental Science › Environmental Chemistry › Methane Hydrates and Related Phenomena
- Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
- Keywords
- Methanopyrus kandleri, piezophiles, cell proliferation, methane production, carbon isotope fractionation, kinetic isotope effects, chemolithoautotrophic bacteria, hydrogenotrophic methanogenesis, high-temperature growth
- MeSH terms
- Base Sequence, Carbon Isotopes, Chemical Fractionation, Kinetics, Methane, Molecular Sequence Data, Pressure, Temperature, Thermodynamics, Survival Analysis, Cell Culture Techniques, Euryarchaeota
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