The DOI matches its Crossref record.
High-resolution mass spectrometry of viral assemblies: Molecular composition and stability of dimorphic hepatitis B virus capsids
Open accessAbstract
Hepatitis B virus (HBV) is a major human pathogen. In addition to its importance in human health, there is growing interest in adapting HBV and other viruses for drug delivery and other nanotechnological applications. In both contexts, precise biophysical characterization of these large macromolecular particles is fundamental. HBV capsids are unusual in that they exhibit two distinct icosahedral geometries, nominally composed of 90 and 120 dimers with masses of ≈3 and ≈4 MDa, respectively. Here, a mass spectrometric approach was used to determine the masses of both capsids to within 0.1%. It follows that both lattices are complete, consisting of exactly 180 and 240 subunits. Nanoindentation experiments by atomic-force microscopy indicate that both capsids have similar stabilities. The data yielded a Young's modulus of ≈0.4 GPa. This experimental approach, anchored on very precise and accurate mass measurements, appears to hold considerable potential for elucidating the assembly of viruses and other macromolecular particles.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Atomic force microscopy-based mechanobiology
Krieg, M., Fläschner, G., Alsteens, D., Gaub, B. M., Roos, W. H., Wuite, G. J. L., Gaub, H. E., Gerber, C., Dufrêne, Y. F., & Müller, D. J. (2018) · Nature Reviews Physics · doi:10.1038/s42254-018-0001-7 · Cited by 799
Self-citationNative mass spectrometry: a bridge between interactomics and structural biology
Heck, A. J. R. (2008) · Nature Methods · doi:10.1038/nmeth.1265 · Cited by 766
Self-citationHigh-Resolution Native Mass Spectrometry
Tamara, S., den Boer, M. A., & Heck, A. J. R. (2022) · Chemical Reviews · doi:10.1021/acs.chemrev.1c00212 · Cited by 511
Self-citationIon mobility mass spectrometry of proteins and proteinassemblies
Uetrecht, C., Rose, R. J., van Duijn, E., Lorenzen, K., & Heck, A. J. R. (2010) · Chem. Soc. Rev. · doi:10.1039/b914002f · Cited by 462
Self-citationInterrogating viral capsid assembly with ion mobility–mass spectrometry
Uetrecht, C., Barbu, I. M., Shoemaker, G. K., van Duijn, E., & Heck, A. J. R. (2011) · Nature Chemistry · doi:10.1038/nchem.947 · Cited by 267
Self-citation
Metadata
- Authors
- Uetrecht, C., Versluis, C., Watts, N. R., Roos, W. H., Wuite, G. J. L., Wingfield, P. T., Steven, A. C., & Heck, A. J. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2008-07-08
- Volume
- 105
- Issue
- 27
- Pages
- 9216-9220
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2093922575
- Cited by
- 219
- References
- 0
- FWCI
- 8.29
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: High-resolution mass spectrometry of viral assemblies: Molecular composition and stability of dimorphic hepatitis B virus capsids. https://app.cochat.ai/papers/10.1073/pnas.0800406105
- The paper
- Uetrecht, C., Versluis, C., Watts, N. R., Roos, W. H., Wuite, G. J. L., Wingfield, P. T., Steven, A. C., & Heck, A. J. R. (2008). High-resolution mass spectrometry of viral assemblies: Molecular composition and stability of dimorphic hepatitis B virus capsids. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0800406105
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
