Cited by 511Mass Spectrometry Techniques and ApplicationsAdvanced Proteomics Techniques and ApplicationsMetabolomics and Mass Spectrometry Studies

High-Resolution Native Mass Spectrometry

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Tamara, Sem, den Boer, Maurits A., Heck, Albert J. R.·2022·Chemical Reviews

Abstract

Native mass spectrometry (MS) involves the analysis and characterization of macromolecules, predominantly intact proteins and protein complexes, whereby as much as possible the native structural features of the analytes are retained. As such, native MS enables the study of secondary, tertiary, and even quaternary structure of proteins and other biomolecules. Native MS represents a relatively recent addition to the analytical toolbox of mass spectrometry and has over the past decade experienced immense growth, especially in enhancing sensitivity and resolving power but also in ease of use. With the advent of dedicated mass analyzers, sample preparation and separation approaches, targeted fragmentation techniques, and software solutions, the number of practitioners and novel applications has risen in both academia and industry. This review focuses on recent developments, particularly in high-resolution native MS, describing applications in the structural analysis of protein assemblies, proteoform profiling of─among others─biopharmaceuticals and plasma proteins, and quantitative and qualitative analysis of protein-ligand interactions, with the latter covering lipid, drug, and carbohydrate molecules, to name a few.

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Crossref · 10.1021/acs.chemrev.1c00212

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  1. McNaught A. D.; Wilkinson A. Compendium of Chemical Terminology: IUPAC Recommendations (The “Gold Book”), 2nd ed.; Blackwell Scientific Publications: Oxford, U.K., 1997.
  2. Mamyrin B. A.; Karataev V. I.; Shmikk D. V.; Zagulin V. A. The Mass-Reflectron. A New Nonmagnetic Time-of-Flight High Resolution Mass-Spectrometer. Zh. Ehksp. Teor. Fiz. 1973, 64, 82– 89.

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Authors
Tamara, S., den Boer, M. A., & Heck, A. J. R.
Affiliations
  • Utrecht University (NL)
  • Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands
Corresponding author
Heck, Albert J. R.
Venue
Chemical Reviews
Published
2021-08-20
Volume
122
Issue
8
Pages
7269-7326
ISSN
  • 0009-2665 (ISSN-L)
  • 1520-6890
OpenAlex
W3194897413
Cited by
511
References
494
Citations by year
2026: 95 · 2025: 138 · 2024: 123 · 2023: 95 · 2022: 54 · 2021: 6
FWCI
7.75
Type
journal article
Language
en
Open access
Locations
  • Chemical Reviews (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • Utrecht University Repository (Utrecht University) (submittedVersion, other-oa)
Funders
Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NACTAR 16442, SPI.2017.028)
Topics
  • Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
  • Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
Keywords
native mass spectrometry, high-resolution mass spectrometry, protein structure, protein complexes, protein assembly, biopharmaceuticals, plasma proteins, protein-ligand interactions, protein-lipid interactions, drug-protein interactions, carbohydrate-protein interactions, mass analyzers
MeSH terms
Ligands, Proteins, Mass Spectrometry, Macromolecular Substances

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