Amorphous calcium phosphate is a major component of the forming fin bones of zebrafish: Indications for an amorphous precursor phase
Open accessAbstract
A fundamental question in biomineralization is the nature of the first-formed mineral phase. In vertebrate bone formation, this issue has been the subject of a long-standing controversy. We address this key issue using the continuously growing fin bony rays of the Tuebingen long-fin zebrafish as a model for bone mineralization. Employing high-resolution scanning and transmission electron microscopy imaging, electron diffraction, and elemental analysis, we demonstrate the presence of an abundant amorphous calcium phosphate phase in the newly formed fin bones. The extracted amorphous mineral particles crystallize with time, and mineral crystallinity increases during bone maturation. Based on these findings, we propose that this amorphous calcium phosphate phase may be a precursor phase that later transforms into the mature crystalline mineral.
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Cryo-EM and HPF-FS preparation have recently provided evidence of a secretory process for bone mineralization.
“Cryo-EM and HPF-FS preparation have recently provided evidence of a secretory process for bone mineralization [131, 133, 154, 155].”
From A Review of Phosphate Mineral Nucleation in Biology and Geobiology (2013) · cites this paper as [154]
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- Authors
- Mahamid, J., Sharir, A., Addadi, L., & Weiner, S.
- Affiliations
- Weizmann Institute of Science (IL)
- Hebrew University of Jerusalem (IL)
- Corresponding author
- Addadi, Lia
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2008-09-02
- Volume
- 105
- Issue
- 35
- Pages
- 12748-12753
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC2529085
- OpenAlex
- W1990454260
- Cited by
- 572
- References
- 1
- Citations by year
- 2026: 16 · 2025: 31 · 2024: 25 · 2023: 29 · 2022: 28 · 2021: 36 · 2020: 39 · 2019: 40 · 2018: 36 · 2017: 23 · 2016: 36 · 2015: 53 · 2014: 39 · 2013: 34 · 2012: 31
- FWCI
- 10.91
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Calcium Carbonate Crystallization and Inhibition
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Alkaline Phosphatase Research Studies
- Health Sciences › Medicine › Rheumatology › Bone and Dental Protein Studies
- Keywords
- amorphous calcium phosphate, biomineralization, bone mineralization, zebrafish, precursor phase, mineral crystallinity, skeletal maturation
- MeSH terms
- Animals, Animal Structures, Calcification, Physiologic, Calcium Phosphates, Male, Osteogenesis, Tomography, X-Ray Computed, Zebrafish, Spectroscopy, Fourier Transform Infrared
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