Cited by 322Bone health and osteoporosis researchBone and Dental Protein StudiesCalcium Carbonate Crystallization and Inhibition

Disaggregation of bone into crystals

Weiner, Stephen, Price, Paul A.·1986·Calcified Tissue International

Abstract

The sizes, shapes, and organizational states of the crystals in bone are studied by systematic disaggregation of the mineral phase. This is achieved by oxidizing the organic phase with sodium hypochlorite, dispersing the resultant particles by sonication, and separating the crystal aggregates from the crystal monomers by gravity setting in ethanol. Six different bones are compared. Bones in which crystals are intimately associated with the collagen fibrils mostly disaggregate into crystal monomers. In dense bones, where the crystals are mostly located between fibrils, they tend to persist as "fused" aggregates. All the crystals are tabular or plate-shaped. In bones in which the majority of crystals are associated with the collagen fibrils, just less than 90% of the crystals are shorter than about 450 A in length. Their widths are on the average about 250 A, almost an order of magnitude larger than the diameters of individual gap regions within the collagen fibril. The notion that one crystal is located in one gap region is therefore untenable and a reevaluation of the relations between collagen and mineral in bone is necessary.

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Crossref · 10.1007/bf02555173

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34 of 34 references verified0 citation errors · 0 not checkable

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  • Nano-sized bone mineral crystals were observed when isolated by the method of Weiner and Price.

    “Nano-sized bone mineral crystals were observed when isolated by the method of Weiner and Price [158].”

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Authors
Weiner, S., & Price, P. A.
Affiliations
University of California San Diego (US)
Venue
Calcified Tissue International
Published
1986-11
Volume
39
Issue
6
Pages
365-375
ISSN
  • 0171-967X (ISSN-L)
  • 1432-0827
OpenAlex
W2030995263
Cited by
322
References
34
Citations by year
2026: 2 · 2025: 7 · 2024: 3 · 2023: 11 · 2022: 9 · 2021: 9 · 2020: 5 · 2019: 10 · 2018: 8 · 2017: 10 · 2016: 11 · 2015: 11 · 2014: 13 · 2013: 14 · 2012: 13
FWCI
11.66
Type
journal article
Language
en
Open access
closed
Locations
  • Calcified Tissue International (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institutes of Health (5r01am027029-06)
Topics
  • Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
  • Health Sciences › Medicine › Rheumatology › Bone and Dental Protein Studies
  • Physical Sciences › Materials Science › Biomaterials › Calcium Carbonate Crystallization and Inhibition
Keywords
collagen fibrils, crystal size and shape, crystal aggregates, bone mineralization, sodium hypochlorite oxidation, ultrasonic dispersion, ethanol precipitation, bone mineral density
MeSH terms
Animals, Bone and Bones, Crystallization, Humans, Hydroxyapatites, Proteins, Specimen Handling, Vertebrates, Durapatite

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