The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
The photorespiratory glycolate metabolism is essential for cyanobacteria and might have been conveyed endosymbiontically to plants
Open accessAbstract
Photorespiratory 2-phosphoglycolate (2PG) metabolism is essential for photosynthesis in higher plants but thought to be superfluous in cyanobacteria because of their ability to concentrate CO 2 internally and thereby inhibit photorespiration. Here, we show that 3 routes for 2PG metabolism are present in the model cyanobacterium Synechocystis sp. strain PCC 6803. In addition to the photorespiratory C2 cycle characterized in plants, this cyanobacterium also possesses the bacterial glycerate pathway and is able to completely decarboxylate glyoxylate via oxalate. A triple mutant with defects in all 3 routes of 2PG metabolism exhibited a high-CO 2 -requiring (HCR) phenotype. All these catabolic routes start with glyoxylate, which can be synthesized by 2 different forms of glycolate dehydrogenase (GlcD). Mutants defective in one or both GlcD proteins accumulated glycolate under high CO 2 level and the double mutant Δ glcD1 /Δ glcD2 was unable to grow under low CO 2 . The HCR phenotype of both the double and the triple mutant could not be attributed to a significantly reduced affinity to CO 2 , such as in other cyanobacterial HCR mutants defective in the CO 2 -concentrating mechanism (CCM). These unexpected findings of an HCR phenotype in the presence of an active CCM indicate that 2PG metabolism is essential for the viability of all organisms that perform oxygenic photosynthesis, including cyanobacteria and C3 plants, at ambient CO 2 conditions. These data and phylogenetic analyses suggest cyanobacteria as the evolutionary origin not only of oxygenic photosynthesis but also of an ancient photorespiratory 2PG metabolism.
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Cites
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“Phylogenetic trees, where the cyanobacterial and corresponding plant proteins cluster closest together, are usually taken as evidence that those genes might have been transferred by means of endosymbiosis into the plant genome (3, 36).”
BackgroundCO2 CONCENTRATING MECHANISMS IN PHOTOSYNTHETIC MICROORGANISMS
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Cited by
Ecological Aspects of the Distribution of Different Autotrophic CO 2 Fixation Pathways
Berg, I. A. (2011) · Applied and Environmental Microbiology · doi:10.1128/aem.02473-10 · Cited by 821
Photorespiration: players, partners and origin
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Mapping photoautotrophic metabolism with isotopically nonstationary 13C flux analysis
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Metadata
- Authors
- Eisenhut, M., Ruth, W., Haimovich, M., Bauwe, H., Kaplan, A., & Hagemann, M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2008-11-04
- Volume
- 105
- Issue
- 44
- Pages
- 17199-17204
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2060800168
- Cited by
- 317
- References
- 39
- FWCI
- 4.96
- Type
- journal article
- Language
- en
- Open access
- green
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- Eisenhut, M., Ruth, W., Haimovich, M., Bauwe, H., Kaplan, A., & Hagemann, M. (2008). The photorespiratory glycolate metabolism is essential for cyanobacteria and might have been conveyed endosymbiontically to plants. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0807043105
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