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Cited by 59Genomics and Chromatin DynamicsDevelopmental Biology and Gene RegulationWound Healing and Treatments

Multiple transcription factor codes activate epidermal wound–response genes in Drosophila

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Pearson, Joseph C., Juarez, Michelle T., Kim, Myungjin, Drivenes, Øyvind, McGinnis, William·2009·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.0810219106PMC PMC2630199
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Abstract

Wounds in Drosophila and mouse embryos induce similar genetic pathways to repair epidermal barriers. However, the transcription factors that transduce wound signals to repair epidermal barriers are largely unknown. We characterize the transcriptional regulatory enhancers of 4 genes-Ddc, ple, msn, and kkv-that are rapidly activated in epidermal cells surrounding wounds in late Drosophila embryos and early larvae. These epidermal wound enhancers all contain evolutionarily conserved sequences matching binding sites for JUN/FOS and GRH transcription factors, but vary widely in trans- and cis-requirements for these inputs and their binding sites. We propose that the combination of GRH and FOS is part of an ancient wound-response pathway still used in vertebrates and invertebrates, but that other mechanisms have evolved that result in similar transcriptional output. A common, but largely untested assumption of bioinformatic analyses of gene regulatory networks is that transcription units activated in the same spatial and temporal patterns will require the same cis-regulatory codes. Our results indicate that this is an overly simplistic view.

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  • The integument cells that are tasked with wound repair and building the outer epidermis of insects and vertebrates use entirely different effector genes, but these genes are driven by the same regulatory factor.

    “Similarly, the integument cells that are tasked with wound repair and building the outer epidermis of insects and vertebrates use entirely different effector genes, but these genes are driven by the same regulatory factor (21).”

    From Transphyletic conservation of developmental regulatory state in animal evolution (2011) · cites this paper as [21]

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Cited by

Sorted by citations

Authors
Pearson, J. C., Juarez, M. T., Kim, M., Drivenes, Ø., & McGinnis, W.
Affiliations
  • University of California San Diego (US)
  • Section of Cell and Developmental Biology, Division of Biology, University of California at San Diego, La Jolla, CA 92093
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
2009-02-17
Volume
106
Issue
7
Pages
2224-2229
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.0810219106
PMC
PMC2630199
OpenAlex
W2122277738
Cited by
59
References
1
Citations by year
2025: 2 · 2024: 1 · 2022: 2 · 2020: 4 · 2019: 2 · 2018: 2 · 2017: 3 · 2016: 2 · 2015: 3 · 2014: 4 · 2013: 6 · 2012: 7
FWCI
3.62
Type
journal article
Language
en
Update notices
correction (2009-11-24)
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • National Institute of General Medical Sciences (R01GM077197, T32GM007240, GM-68524, K12GM068524)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
Keywords
transcription factor binding sites, enhancer regulation, Drosophila embryo, DDC, PLE, MSN, Fos/Jun, gene regulatory networks
MeSH terms
Animals, Binding Sites, Drosophila, Drosophila melanogaster, Enhancer Elements, Genetic, Epidermis, Gene Expression Regulation, Microscopy, Fluorescence, Models, Biological, Models, Genetic, Mutation, Time Factors, Transcription Factors, Transcription, Genetic, Wound Healing

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