Fully functional bioengineered tooth replacement as an organ replacement therapy
Open accessAbstract
Current approaches to the development of regenerative therapies have been influenced by our understanding of embryonic development, stem cell biology, and tissue engineering technology. The ultimate goal of regenerative therapy is to develop fully functioning bioengineered organs which work in cooperation with surrounding tissues to replace organs that were lost or damaged as a result of disease, injury, or aging. Here, we report a successful fully functioning tooth replacement in an adult mouse achieved through the transplantation of bioengineered tooth germ into the alveolar bone in the lost tooth region. We propose this technology as a model for future organ replacement therapies. The bioengineered tooth, which was erupted and occluded, had the correct tooth structure, hardness of mineralized tissues for mastication, and response to noxious stimulations such as mechanical stress and pain in cooperation with other oral and maxillofacial tissues. This study represents a substantial advance and emphasizes the potential for bioengineered organ replacement in future regenerative therapies.
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In animal models, rodent and human skin with hair follicles can be reconstituted by co-culturing mesenchymal cells with epithelial cells or HF-initiating dermal papilla cells.
“In animal models, rodent and human skin with HFs can be reconstituted by co-culturing mesenchymal cells with epithelial cells or, specifically, HF initiating dermal papilla (DP) cells (Asakawa et al., 2012; Chuong et al., 2007; Ehama et al., 2007; Ikeda et al., 2009; Nakao et al., 2007; Takagi et al., 2016; Toyoshima et al., 2012; Zheng et al., 2005a).”
From Hair Follicle Development in Mouse Pluripotent Stem Cell-Derived Skin Organoids (2018) · cites this paper as Ikeda et al., 2009
The paper’s introductory section says epithelial and mesenchymal cells can be used to reconstitute organ germs and identifies the whisker follicle as a possible application. It does not report reconstituting rodent or human skin with hair follicles, or describe co-culture with hair-follicle-initiating dermal papilla cells; its reported replacement experiment concerns teeth. (LLM, conf=0.79)
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Cites
Hematopoietic Stem-Cell Transplantation
Copelan, E. A. (2006) · New England Journal of Medicine · doi:10.1056/nejmra052638 · Cited by 2,522 · see citing works
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Research works printed but not linked: 1
- [44]Voruganti K (2008) Clinical periodontology and implant dentistry, 5th edition. Br Dent J 205:216.
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Fully functional hair follicle regeneration through the rearrangement of stem cells and their niches
Toyoshima, K.-E., Asakawa, K., Ishibashi, N., Toki, H., Ogawa, M., Hasegawa, T., Irié, T., Tachikawa, T., Sato, A., Takeda, A., et al. (2012) · Nature Communications · doi:10.1038/ncomms1784 · Cited by 250 · see citing works
Self-citationHair Follicle Development in Mouse Pluripotent Stem Cell-Derived Skin Organoids
Lee, J., Bӧscke, R., Tang, P.-C., Hartman, B. H., Heller, S., & Koehler, K. R. (2018) · Cell Reports · doi:10.1016/j.celrep.2017.12.007 · Cited by 187 · see citing works
- Authors
- Ikeda, E., Morita, R., Nakao, K., Ishida, K., Nakamura, T., Takano-Yamamoto, T., Ogawa, M., Mizuno, M., Kasugai, S., & Tsuji, T.
- Affiliations
- Tokyo University of Science (JP)
- Division of Orthodontics and Dentofacial Orthopedics, Graduate School of Dentistry, Tohoku University, Sendai, Miyagi 980-8575, Japan; and
- Organ Technologies (Japan) (JP)
- Tokyo Medical and Dental University (JP)
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2009-08-11
- Volume
- 106
- Issue
- 32
- Pages
- 13475-13480
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC2720406
- OpenAlex
- W2104831977
- Cited by
- 447
- References
- 1
- Citations by year
- 2026: 6 · 2025: 18 · 2024: 14 · 2023: 17 · 2022: 17 · 2021: 23 · 2020: 26 · 2019: 25 · 2018: 30 · 2017: 38 · 2016: 40 · 2015: 33 · 2014: 37 · 2013: 35 · 2012: 42
- FWCI
- 11.82
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › dental development and anomalies
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Keywords
- tooth germ transplantation, organ replacement therapy, regenerative therapy, alveolar bone, tooth eruption, tooth mineralization, mechanical stress response, tooth structure, tooth loss
- MeSH terms
- Animals, Hardness, Mastication, Mice, Inbred C57BL, Neurons, Pain, Stress, Mechanical, Tooth, Tooth Eruption, Bioartificial Organs, Tissue Engineering, Regenerative Medicine, Mice
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