Fully functional hair follicle regeneration through the rearrangement of stem cells and their niches
Open accessAbstract
Organ replacement regenerative therapy is purported to enable the replacement of organs damaged by disease, injury or aging in the foreseeable future. Here we demonstrate fully functional hair organ regeneration via the intracutaneous transplantation of a bioengineered pelage and vibrissa follicle germ. The pelage and vibrissae are reconstituted with embryonic skin-derived cells and adult vibrissa stem cell region-derived cells, respectively. The bioengineered hair follicle develops the correct structures and forms proper connections with surrounding host tissues such as the epidermis, arrector pili muscle and nerve fibres. The bioengineered follicles also show restored hair cycles and piloerection through the rearrangement of follicular stem cells and their niches. This study thus reveals the potential applications of adult tissue-derived follicular stem cells as a bioengineered organ replacement therapy. Bioengineered hair follicles can be produced from embryonic follicle germ cells, but whether these follicles can interact with the surrounding tissue and function normally is unknown. Here, bioengineered hair follicles transplanted into mouse dermis make connections with the surrounding tissue and show normal hair cycles.
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HFG-like aggregates were reconstructed in culture using dissociated embryonic epithelial and mesenchymal cells.
“Hair follicle germ (HFG)–like aggregates were reconstructed in culture using dissociated embryonic epithelial and mesenchymal cells (10).”
From Reprogramming of three-dimensional microenvironments for in vitro hair follicle induction (2022) · cites this paper as [10]
When transplanted into the skin of nude mice, HFGs generated de novo hair follicles, implicating that HFGs have hair neogenesis capability.
“When transplanted into the skin of nude mice, HFGs generated de novo hair follicles, implicating that HFGs have hair neogenesis capability.”
From Reprogramming of three-dimensional microenvironments for in vitro hair follicle induction (2022) · cites this paper as [10]
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Self-citation
- Authors
- Toyoshima, K.-E., Asakawa, K., Ishibashi, N., Toki, H., Ogawa, M., Hasegawa, T., Irié, T., Tachikawa, T., Sato, A., Takeda, A., & Tsuji, T.
- Affiliations
- Tokyo University of Science (JP)
- Research Institute for Science and Technology, Tokyo University of Science (JP)
- Organ Technologies (Japan) (JP)
- SHOWA Medical University (JP)
- Corresponding author
- Tsuji, Takashi
- Venue
- Nature Communications
- Published
- 2012-04-17
- Volume
- 3
- Issue
- 1
- Pages
- 784-784
- ISSN
- 2041-1723 (ISSN-L)
- PMC
- PMC3337983
- OpenAlex
- W2164589411
- Cited by
- 250
- References
- 62
- Citations by year
- 2026: 18 · 2025: 10 · 2024: 14 · 2023: 18 · 2022: 22 · 2021: 21 · 2020: 17 · 2019: 13 · 2018: 12 · 2017: 29 · 2016: 18 · 2015: 16 · 2014: 14 · 2013: 20 · 2012: 7
- FWCI
- 20.98
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Nature Communications (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Funders
- Japan Society for the Promotion of Science (22249064)
- Topics
- Health Sciences › Medicine › Urology › Hair Growth and Disorders
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
- Keywords
- hair follicle regeneration, stem cell niche, organ replacement therapy, hair cycle, piloerection, arrector pili muscle, nerve innervation, tissue engineering
- MeSH terms
- Adult, Animals, Cell Differentiation, Humans, Male, Mice, Inbred BALB C, Mice, Inbred C57BL, Middle Aged, Vibrissae, Hair Follicle, Cell Proliferation, Mice, Adult Stem Cells, Bioengineering
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