The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.
A microRNA regulatory mechanism of osteoblast differentiation
Open accessAbstract
Growing evidence shows that microRNAs (miRNAs) regulate various developmental and homeostatic events in vertebrates and invertebrates. Osteoblast differentiation is a key step in proper skeletal development and acquisition of bone mass; however, the physiological role of non-coding small RNAs, especially miRNAs, in osteoblast differentiation remains elusive. Here, through comprehensive analysis of miRNAs expression during osteoblast differentiation, we show that miR-206, previously viewed as a muscle-specific miRNA, is a key regulator of this process. miR-206 was expressed in osteoblasts, and its expression decreased over the course of osteoblast differentiation. Overexpression of miR-206 in osteoblasts inhibited their differentiation, and conversely, knockdown of miR-206 expression promoted osteoblast differentiation. In silico analysis and molecular experiments revealed connexin 43 (Cx43), a major gap junction protein in osteoblasts, as a target of miR-206, and restoration of Cx43 expression in miR-206-expressing osteoblasts rescued them from the inhibitory effect of miR-206 on osteoblast differentiation. Finally, transgenic mice expressing miR-206 in osteoblasts developed a low bone mass phenotype due to impaired osteoblast differentiation. Our data show that miRNA is a regulator of osteoblast differentiation.
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Cited by
Osteoclast-derived exosomal miR-214-3p inhibits osteoblastic bone formation
Li, D., Liu, J., Guo, B., Liang, C., Dang, L., Lu, C., He, X., Cheung, H. Y.-S., Xu, L., Lu, C., et al. (2016) · Nature Communications · doi:10.1038/ncomms10872 · Cited by 588
miR-214 targets ATF4 to inhibit bone formation
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miR-29 Modulates Wnt Signaling in Human Osteoblasts through a Positive Feedback Loop
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Nielsen, M. S., Nygaard Axelsen, L., Sorgen, P. L., Verma, V., Delmar, M., & Holstein‐Rathlou, N. (2012) · Comprehensive Physiology · doi:10.1002/cphy.c110051 · Cited by 386
Metadata
- Authors
- Inose, H., Ochi, H., Kimura, A., Fujita, K., Xu, R., Sato, S., Iwasaki, M., Sunamura, S., Takeuchi, Y., Fukumoto, S., Saito, K., Nakamura, T., Siomi, H., Ito, H., Arai, Y., Shinomiya, K.-I., & Takeda, S.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2009-12-08
- Volume
- 106
- Issue
- 49
- Pages
- 20794-20799
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2089125941
- Cited by
- 295
- References
- 47
- FWCI
- 6.48
- Type
- journal article
- Language
- en
- Open access
- green
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- Inose, H., Ochi, H., Kimura, A., Fujita, K., Xu, R., Sato, S., Iwasaki, M., Sunamura, S., Takeuchi, Y., Fukumoto, S., Saito, K., Nakamura, T., Siomi, H., Ito, H., Arai, Y., Shinomiya, K.-I., & Takeda, S. (2009). A microRNA regulatory mechanism of osteoblast differentiation. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0909311106
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