The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Extensive phosphorylation with overlapping specificity by Mycobacterium tuberculosis serine/threonine protein kinases
Open accessAbstract
The Mycobacterium tuberculosis genome encodes 11 serine/threonine protein kinases (STPKs) that are structurally related to eukaryotic kinases. To gain insight into the role of Ser/Thr phosphorylation in this major global pathogen, we used a phosphoproteomic approach to carry out an extensive analysis of protein phosphorylation in M. tuberculosis . We identified more than 500 phosphorylation events in 301 proteins that are involved in a broad range of functions. Bioinformatic analysis of quantitative in vitro kinase assays on peptides containing a subset of these phosphorylation sites revealed a dominant motif shared by six of the M. tuberculosis STPKs. Kinase assays on a second set of peptides incorporating targeted substitutions surrounding the phosphoacceptor validated this motif and identified additional residues preferred by individual kinases. Our data provide insight into processes regulated by STPKs in M. tuberculosis and create a resource for understanding how specific phosphorylation events modulate protein activity. The results further provide the potential to predict likely cognate STPKs for newly identified phosphoproteins.
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Beausoleil, S. A., Villén, J., Gerber, S. A., Rush, J., & Gygi, S. P. (2006) · Nature Biotechnology · doi:10.1038/nbt1240 · Cited by 1,577
Kang, C.-M., Abbott, D. W., Park, S. T., Dascher, C. C., Cantley, L. C., & Husson, R. N. (2005) · Genes & Development · doi:10.1101/gad.1311105 · Cited by 388
Self-citationKang, C.-M., Nyayapathy, S., Lee, J.-Y., Suh, J.-W., & Husson, R. N. (2008) · Microbiology · doi:10.1099/mic.0.2007/014076-0 · Cited by 264
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Cited by
Xie, L., Liu, W., Li, Q., Chen, S., Xu, M., Huang, Q., Zeng, J., Zhou, M., & Xie, J. (2015) · Journal of Proteome Research · doi:10.1021/pr500859a · Cited by 128
Global Dynamics of the Escherichia coli Proteome and Phosphoproteome During Growth in Minimal Medium
Soares, N. C., Spät, P., Krug, K., & Macek, B. (2013) · Journal of Proteome Research · doi:10.1021/pr3011843 · Cited by 113
Phosphorylation of KasB Regulates Virulence and Acid-Fastness in Mycobacterium tuberculosis
Vilchèze, C., Molle, V., Carrère-Kremer, S., Leiba, J., Mourey, L., Shenai, S., Baronian, G., Tufariello, J., Hartman, T., Veyron-Churlet, R., et al. (2014) · PLoS Pathogens · doi:10.1371/journal.ppat.1004115 · Cited by 76
Mycobacterium tuberculosis Ser/Thr Protein Kinase B Mediates an Oxygen-Dependent Replication Switch
Ortega, C., Liao, R., Anderson, L. N., Rustad, T., Ollodart, A. R., Wright, A. T., Sherman, D. R., & Grundner, C. (2014) · PLoS Biology · doi:10.1371/journal.pbio.1001746 · Cited by 74
Carette, X., Platig, J., Young, D. C., Helmel, M., Young, A. T., Wang, Z., Potluri, L.-P., Moody, C. S., Zeng, J., Prisic, S., et al. (2018) · mBio · doi:10.1128/mbio.02333-17 · Cited by 65
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Metadata
- Authors
- Prisic, S., Dankwa, S., Schwartz, D., Chou, M. F., Locasale, J. W., Kang, C.-M., Bemis, G., Church, G. M., Steen, H., & Husson, R. N.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2010-04-05
- Volume
- 107
- Issue
- 16
- Pages
- 7521-7526
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2064910808
- Cited by
- 268
- References
- 3
- FWCI
- 12.11
- Type
- journal article
- Language
- en
- Open access
- green
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- Prisic, S., Dankwa, S., Schwartz, D., Chou, M. F., Locasale, J. W., Kang, C.-M., Bemis, G., Church, G. M., Steen, H., & Husson, R. N. (2010). Extensive phosphorylation with overlapping specificity by Mycobacterium tuberculosis serine/threonine protein kinases. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0913482107
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