The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Genetic dissection of polyunsaturated fatty acid synthesis in Caenorhabditis elegans
Open accessAbstract
Polyunsaturated fatty acids (PUFAs) are important membrane components and precursors of signaling molecules. To investigate the roles of these fatty acids in growth, development, and neurological function in an animal system, we isolated Caenorhabditis elegans mutants deficient in PUFA synthesis by direct analysis of fatty acid composition. C. elegans possesses all the desaturase and elongase activities to synthesize arachidonic acid and eicosapentaenoic acid from saturated fatty acid precursors. In our screen we identified mutants with defects in each fatty acid desaturation and elongation step of the PUFA biosynthetic pathway. The fatty acid compositions of the mutants reveal the substrate preferences of the desaturase and elongase enzymes and clearly demarcate the steps of this pathway. The mutants show that C. elegans does not require n3 or Δ5-unsaturated PUFAs for normal development under laboratory conditions. However, mutants with more severe PUFA deficiencies display growth and neurological defects. The mutants provide tools for investigating the roles of PUFAs in membrane biology and cell function in this animal model.
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Prostaglandins and Leukotrienes: Advances in Eicosanoid Biology
Funk, C. D. (2001) · Science · doi:10.1126/science.294.5548.1871 · Cited by 3,817
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An ARC/Mediator subunit required for SREBP control of cholesterol and lipid homeostasis
Yang, F., Vought, B. W., Satterlee, J. S., Walker, A. K., Jim Sun, Z.-Y., Watts, J. L., DeBeaumont, R., Mako Saito, R., Hyberts, S. G., Yang, S., et al. (2006) · Nature · doi:10.1038/nature04942 · Cited by 405
Self-citationBergé, J.-P., & Barnathan, G. (2005) · Advances in Biochemical Engineering/Biotechnology · doi:10.1007/b135782 · Cited by 401
Polyunsaturated fatty acid synthesis: What will they think of next?
Wallis, J. G., Watts, J. L., & Browse, J. (2002) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(02)02168-0 · Cited by 375
Polyunsaturated Fatty Acids Drive Lipid Peroxidation during Ferroptosis
Mortensen, M. S., Ruiz, J., & Watts, J. L. (2023) · Cells · doi:10.3390/cells12050804 · Cited by 312
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Metadata
- Authors
- Watts, J. L., & Browse, J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2002-04-23
- Volume
- 99
- Issue
- 9
- Pages
- 5854-5859
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2004775480
- Cited by
- 402
- References
- 1
- FWCI
- 4.09
- Type
- journal article
- Language
- en
- Open access
- green
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