The DOI matches its Crossref record.
Fast live simultaneous multiwavelength four-dimensional optical microscopy
Open accessAbstract
Live fluorescence microscopy has the unique capability to probe dynamic processes, linking molecular components and their localization with function. A key goal of microscopy is to increase spatial and temporal resolution while simultaneously permitting identification of multiple specific components. We demonstrate a new microscope platform, OMX, that enables subsecond, multicolor four-dimensional data acquisition and also provides access to subdiffraction structured illumination imaging. Using this platform to image chromosome movement during a complete yeast cell cycle at one 3D image stack per second reveals an unexpected degree of photosensitivity of fluorophore-containing cells. To avoid perturbation of cell division, excitation levels had to be attenuated between 100 and 10,000× below the level normally used for imaging. We show that an image denoising algorithm that exploits redundancy in the image sequence over space and time allows recovery of biological information from the low light level noisy images while maintaining full cell viability with no fading.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Phototoxicity in live fluorescence microscopy, and how to avoid it
Icha, J., Weber, M., Waters, J. C., & Norden, C. (2017) · BioEssays · doi:10.1002/bies.201700003 · Cited by 622
Live-cell imaging reveals enhancer-dependent Sox2 transcription in the absence of enhancer proximity
Alexander, J. M., Guan, J., Li, B., Maliskova, L., Song, M., Shen, Y., Huang, B., Lomvardas, S., & Weiner, O. D. (2019) · eLife · doi:10.7554/elife.41769 · Cited by 346
The Conserved GTPase Gem1 Regulates Endoplasmic Reticulum–Mitochondria Connections
Kornmann, B., Osman, C., & Walter, P. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1111314108 · Cited by 335
Tracking single molecules at work in living cells
Kusumi, A., Tsunoyama, T. A., Hirosawa, K. M., Kasai, R. S., & Fujiwara, T. K. (2014) · Nature Chemical Biology · doi:10.1038/nchembio.1558 · Cited by 332
Murley, A., Lackner, L. L., Osman, C., West, M., Voeltz, G. K., Walter, P., & Nunnari, J. (2013) · eLife · doi:10.7554/elife.00422 · Cited by 326
Metadata
- Authors
- Carlton, P. M., Boulanger, J., Kervrann, C., Sibarita, J.-B., Salamero, J., Gordon-Messer, S., Bressan, D., Haber, J. E., Haase, S., Shao, L., Winoto, L., Matsuda, A., Kner, P., Uzawa, S., Gustafsson, M., Kam, Z., Agard, D. A., & Sedat, J. W.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2010-08-12
- Volume
- 107
- Issue
- 37
- Pages
- 16016-16022
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2085617302
- Cited by
- 186
- References
- 0
- FWCI
- 7.10
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Fast live simultaneous multiwavelength four-dimensional optical microscopy. https://app.cochat.ai/papers/10.1073/pnas.1004037107
- The paper
- Carlton, P. M., Boulanger, J., Kervrann, C., Sibarita, J.-B., Salamero, J., Gordon-Messer, S., Bressan, D., Haber, J. E., Haase, S., Shao, L., Winoto, L., Matsuda, A., Kner, P., Uzawa, S., Gustafsson, M., Kam, Z., Agard, D. A., & Sedat, J. W. (2010). Fast live simultaneous multiwavelength four-dimensional optical microscopy. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1004037107
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
