The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index; none of the 2 sources its claims cite is checked for support yet.
Dietary inorganic nitrate reverses features of metabolic syndrome in endothelial nitric oxide synthase-deficient mice
Open accessAbstract
The metabolic syndrome is a clustering of risk factors of metabolic origin that increase the risk for cardiovascular disease and type 2 diabetes. A proposed central event in metabolic syndrome is a decrease in the amount of bioavailable nitric oxide (NO) from endothelial NO synthase (eNOS). Recently, an alternative pathway for NO formation in mammals was described where inorganic nitrate, a supposedly inert NO oxidation product and unwanted dietary constituent, is serially reduced to nitrite and then NO and other bioactive nitrogen oxides. Here we show that several features of metabolic syndrome that develop in eNOS-deficient mice can be reversed by dietary supplementation with sodium nitrate, in amounts similar to those derived from eNOS under normal conditions. In humans, this dose corresponds to a rich intake of vegetables, the dominant dietary nitrate source. Nitrate administration increased tissue and plasma levels of bioactive nitrogen oxides. Moreover, chronic nitrate treatment reduced visceral fat accumulation and circulating levels of triglycerides and reversed the prediabetic phenotype in these animals. In rats, chronic nitrate treatment reduced blood pressure and this effect was also present during NOS inhibition. Our results show that dietary nitrate fuels a nitrate–nitrite–NO pathway that can partly compensate for disturbances in endogenous NO generation from eNOS. These findings may have implications for novel nutrition-based preventive and therapeutic strategies against cardiovascular disease and type 2 diabetes.
Overview
AI snapshot
Written from the paper’s full text.
TL;DR
Dietary sodium nitrate supplementation in eNOS-deficient mice increased bioactive nitrogen oxides and reversed several features of metabolic syndrome, including visceral fat, circulating triglycerides, and a prediabetic glucose phenotype. Chronic nitrate also lowered blood pressure in rats, with the effect still present during NOS inhibition.
Key findings
- Acute nitrate (0.1 mmol·kg−1 i.p.) increased plasma nitrite and tissue nitros(yl)ation products in eNOS-deficient mice; chronic nitrate increased tissue S-nitrosothiols/nitros(yl)ation products without significantly changing plasma/tissue nitrate and nitrite versus controls.
- In aged eNOS−/− mice, chronic sodium nitrate (0.1 mmol·kg−1·d−1; 85 mg·L−1, 1 mM in drinking water for 8–10 wk) reduced body weight over a 7-wk observation period, reduced visceral fat, and lowered circulating triglycerides.
- After 10 wk of nitrate supplementation, glucose tolerance was almost normalized in eNOS−/− mice; fasting blood glucose, HbA1c, and the proinsulin/insulin ratio were lower than in untreated eNOS−/− mice. Nitrate had no effect on glucose tolerance in young wild-type or nNOS-deficient mice.
- In rats, chronic nitrate (same dose for 8 wk) lowered mean arterial pressure throughout a 3-d observation period, and this difference persisted after L-NAME (1 g·L−1); L-NAME’s pressor effect was delayed by 12 h in nitrate-treated animals. No firm evidence of increased mitochondrial biogenesis or tissue cGMP was found.
Methods
- Aged eNOS-deficient mice (mean 16 mo, range 14–22 mo) were randomized to sodium nitrate in drinking water (85 mg·L−1, 1 mM) for 8–10 wk or control; acute experiments used 0.1 mmol·kg−1 i.p. Rats received the same nitrate dose for 8 wk for telemetric blood pressure measurements, followed by L-NAME (1 g·L−1).
- Nitrate, nitrite, and nitros(yl)ation products were measured by chemiluminescence. Glucose tolerance tests were performed after 14 h fasting with i.p. glucose (2 g·kg−1); HbA1c, insulin, and proinsulin were measured after 14 h fasting. Body weight was recorded weekly for 7 wk; visceral adipose tissue and triglycerides were measured at termination.
- Statistics used mean ± SEM with 5–15 animals per group; two-tailed independent Student’s t test, ANOVA with Bonferroni or Dunnett’s post hoc tests, and P < 0.05 considered significant.
Limitations
- The paper states that the reason for the 12-h delay in the L-NAME blood-pressure response in nitrate-treated rats is not known, and that the importance of enterosalivary nitrate cycling and bacteria-derived nitrite formation in mice is yet to be determined.
- The paper states that long-term human intervention studies are warranted and that the exact mechanisms underlying metabolic syndrome remain unsettled; it also notes that failure to detect increased tissue cGMP does not exclude cGMP-mediated effects.
- Evident from the text: findings are from animal models (aged eNOS-deficient mice and rats), not humans; sample sizes were modest (5–15 per group), and the eNOS-deficient model may not fully represent human metabolic syndrome.
Model-written summary. Check it against the paper before relying on it.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
0 of 2 cited sources checked · 0 supported · 0 flagged
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Here we show that several features of metabolic syndrome that develop in eNOS-deficient mice can be reversed by dietary supplementation with sodium nitrate, in amounts similar to those derived from eNOS under normal conditions.”
- Also stated in: § Discussion · plain
“The results presented herein show that dietary supplementation with inorganic nitrate attenuates several features of metabolic syndrome in aged eNOS-deficient mice.”
§ Dietary inorganic nitrate reverses features of metabolic syndrome in endothelial nitric oxide synthase-deficient mice > Abstract
Tests✓ Asserted: passes✓ Checkable: passes✓ Load-bearing: passes✓ Based: passes- Rests on an assumption: The effects of sodium nitrate are attributable to nitrate rather than to sodium or other components of the treatment.
- Also stated in: § Discussion · plain
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“With chronic low-dose administration of nitrate, plasma and tissue levels of nitrate and nitrite were not significantly different from those seen in control animals receiving no nitrate supplementation.”
§ Dietary inorganic nitrate reverses features of metabolic syndrome in endothelial nitric oxide synthase-deficient mice > Results > Formation of Bioactive Nitrogen Oxides from Dietary Nitrate.
Load-bearingStrength:plain· not yet calibratedTests✓ Asserted: passes✓ Checkable: passes✓ Load-bearing: passes✓ Based: passes- Rests on an assumption: The effects of sodium nitrate are attributable to nitrate rather than to sodium or other components of the treatment.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“This finding indicates that at least some of the observed metabolic effects of nitrate are cGMP-independent.”
§ Dietary inorganic nitrate reverses features of metabolic syndrome in endothelial nitric oxide synthase-deficient mice > Discussion
Tests✓ Asserted: passes✓ Checkable: passes✓ Load-bearing: passes✓ Based: passesA conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“The present findings are highly relevant from a nutritional perspective as well, as the amount of nitrate used is readily achievable via a normal diet.”
§ Dietary inorganic nitrate reverses features of metabolic syndrome in endothelial nitric oxide synthase-deficient mice > Discussion
Load-bearingStrength:plain· not yet calibratedTests✓ Asserted: passes✓ Checkable: passes✓ Load-bearing: passes✓ Based: passesA conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Thus, in addition to the second-by-second regulation of vascular tone by eNOS-derived NO, its oxidized end-product nitrate may serve as a long-lived reservoir for NO-like bioactivity in tissues.”
§ Dietary inorganic nitrate reverses features of metabolic syndrome in endothelial nitric oxide synthase-deficient mice > Discussion
Tests✓ Asserted: passes✓ Checkable: passes✓ Load-bearing: passes✓ Based: passes
- It may state two separate claims as one. 4
- Probably not a substantial claim. 4
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“In the present study, we failed to detect an increase in tissue cGMP formation after nitrate, but we did detect significant formation of nitros(yl)ation products, including S-nitrosothiols, in the tissues after nitrate administration.”
§ Dietary inorganic nitrate reverses features of metabolic syndrome in endothelial nitric oxide synthase-deficient mice > Discussion
Load-bearingStrength:plain· not yet calibratedTests✓ Asserted: passes✓ Checkable: passes✓ Load-bearing: passes✓ Based: passesIt may state two separate claims as one.
- Rests on an assumption: The effects of sodium nitrate are attributable to nitrate rather than to sodium or other components of the treatment.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“The fact that this very modest amount had such profound biological effects supports the intriguing possibility that endogenous nitrate levels are already sufficient to affect cellular processes.”
§ Dietary inorganic nitrate reverses features of metabolic syndrome in endothelial nitric oxide synthase-deficient mice > Discussion
Tests✓ Asserted: passes✓ Checkable: passes✓ Load-bearing: passes✓ Based: passesIt may state two separate claims as one.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“The reason for this is not known, but apparently NOS-independent NO formation seemed to have prevented the initial blood-pressure response during NOS inhibition.”
§ Dietary inorganic nitrate reverses features of metabolic syndrome in endothelial nitric oxide synthase-deficient mice > Results > Blood Pressure.
Strength:hedged· not yet calibratedCausal:conditional· not yet calibratedTests✓ Asserted: passes✓ Checkable: passes✗ Load-bearing: fails✓ Based: passesProbably not a substantial claim. The paper’s conclusions may not depend on it.
What the paper did — it cites no source for this, so there is none to check. The quote shows it is in the paper.
In the paper
“The eNOS-deficient mice were obtained from Jackson Laboratories and were randomly assigned to treatment groups to ensure that each group had the same average age (mean 16 mo, range 14–22 mo) and weight.”
§ Dietary inorganic nitrate reverses features of metabolic syndrome in endothelial nitric oxide synthase-deficient mice > Materials and Methods > Animals.
Strength:plain· not yet calibratedCausal:direct· not yet calibratedTests✓ Asserted: passes✓ Checkable: passes✗ Load-bearing: fails✓ Based: passesProbably not a substantial claim. The paper’s conclusions may not depend on it.
What the paper did — it cites no source for this, so there is none to check. The quote shows it is in the paper.
In the paper
“All animal work was conducted in accordance with the Swedish Animal Research Committee at Karolinska Institutet.”
§ Dietary inorganic nitrate reverses features of metabolic syndrome in endothelial nitric oxide synthase-deficient mice > Materials and Methods > Animals.
Strength:plain· not yet calibratedTests✓ Asserted: passes✓ Checkable: passes✗ Load-bearing: fails✓ Based: passesProbably not a substantial claim. The paper’s conclusions may not depend on it.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“This result is consistent with the theory that metabolic effects of nitrate might be related to a reduction in oxidative stress, which is indeed absent or less pronounced in these mice.”
§ Dietary inorganic nitrate reverses features of metabolic syndrome in endothelial nitric oxide synthase-deficient mice > Discussion
Tests✓ Asserted: passes✓ Checkable: passes✓ Load-bearing: passes✓ Based: passesIt may state two separate claims as one.
None of the sources cited for this claim have been checked yet.
In the paper
“Ingested nitrate is rapidly absorbed in the small intestine, then actively taken up by the salivary glands and concentrated in saliva.”
§ Dietary inorganic nitrate reverses features of metabolic syndrome in endothelial nitric oxide synthase-deficient mice > Discussion
What the sources say
- Not checked yetcites [18] Lundberg JO, Govoni M. Inorganic nitrate is a possible source for systemic generation of nitric oxide. Free Radic Biol Med. 2004;37:395–400. doi: 10.1016/j.freeradbiomed.2004.04.027. [[DOI](https://doi.org/10.1016/j.freeradbiomed.2004.04.027)] [[PubMed](https://pubmed.ncbi.nlm.nih.gov/15223073/)] [[Google Scholar](https://scholar.google.com/scholar_lookup?journal=Free%20Radic%20Biol%20Med&title=Inorganic%20nitrate%20is%20a%20possible%20source%20for%20systemic%20generation%20of%20nitric%20oxide&author=JO%20Lundberg&author=M%20Govoni&volume=37&publication_year=2004&pages=395-400&pmid=15223073&do source
- Not checked yetcites [22] Gladwin MT, et al. The emerging biology of the nitrite anion. Nat Chem Biol. 2005;1:308–314. doi: 10.1038/nchembio1105-308. [[DOI](https://doi.org/10.1038/nchembio1105-308)] [[PubMed](https://pubmed.ncbi.nlm.nih.gov/16408064/)] [[Google Scholar](https://scholar.google.com/scholar_lookup?journal=Nat%20Chem%20Biol&title=The%20emerging%20biology%20of%20the%20nitrite%20anion&author=MT%20Gladwin&volume=1&publication_year=2005&pages=308-314&pmid=16408064&doi=10.1038/nchembio1105-308&)] source
Strength:plain· not yet calibratedCausal:direct· not yet calibratedTests✓ Asserted: passes✓ Checkable: passes✗ Load-bearing: fails✓ Based: passesProbably not a substantial claim. The paper’s conclusions may not depend on it. It may state two separate claims as one.
The paper relies on this without support.
The effects of sodium nitrate are attributable to nitrate rather than to sodium or other components of the treatment.
“NaNO3 was added to the drinking water during 8 to 10 wk at a concentration of 85 mg·L−1 (1 mM).”
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“the therapeutic potential of dietary nitrate/nitrite has been supported by recent studies demonstrating the improvements of insulin resistance in humans and animals as a result of its enhancing the NO availability in plasma and tissues [65,66,67,68].”
From NO-Rich Diet for Lifestyle-Related Diseases (2015) · cites this paper as [66]
In the citing paper
“the endogenous production of nitrate from NO oxidation (mainly via reaction with oxy-hemoglobin) has long been recognized to be an order of magnitude greater than dietary intake as shown in the late 1970s [7] and more recently in studies using eNOS-deficient mice [45].”
From Nitrate reduction to nitrite, nitric oxide and ammonia by gut bacteria under physiological conditions (2015) · cites this paper as [45]
In the citing paper
“NOS3 dependent NO production plays an important role in regulating steady state plasma nitrite levels, while also influencing nitrate, nitroso, and nitrosyl products in tissues [72, 73].”
From Inorganic nitrite therapy: historical perspective and future directions (2011) · cites this paper as [72]
In the citing paper
“Recent work from Carlström et al reveals that long term dietary nitrate consumption can reverse metabolic parameters associated with eNOS dysfunction that are associated with the development of cardiovascular disease [72].”
From Inorganic nitrite therapy: historical perspective and future directions (2011) · cites this paper as [72]
In the citing paper
“It was found that daily administration of 85 mg/L−1 in the drinking water for 8–10 weeks decreased body weight, plasma triglycerides, and visceral fat while improving glucose tolerance and fasting blood glucose levels in eNOS deficient mice.”
From Inorganic nitrite therapy: historical perspective and future directions (2011) · cites this paper as [72]
Citations
Cites
An obesity-associated gut microbiome with increased capacity for energy harvest
Turnbaugh, P. J., Ley, R. E., Mahowald, M. A., Magrini, V., Mardis, E. R., & Gordon, J. I. (2006) · Nature · doi:10.1038/nature05414 · Cited by 12,444
The L-Arginine-Nitric Oxide Pathway
Epstein, F. H., Moncada, S., & Higgs, A. (1993) · New England Journal of Medicine · doi:10.1056/nejm199312303292706 · Cited by 6,678
Eckel, R. H., Alberti, K., Grundy, S. M., & Zimmet, P. (2010) · The Lancet · doi:10.1016/s0140-6736(09)61794-3 · Cited by 6,317
Eckel, R. H., Grundy, S. M., & Zimmet, P. (2005) · The Lancet · doi:10.1016/s0140-6736(05)66378-7 · Cited by 5,755
Abdominal obesity and metabolic syndrome
Després, J.-P., & Lemieux, I. (2006) · Nature · doi:10.1038/nature05488 · Cited by 4,354
The nitrate-nitrite-nitric oxide pathway in physiology and therapeutics
Lundberg, J. O., Weitzberg, E., & Gladwin, M. T. (2008) · Nature Reviews Drug Discovery · doi:10.1038/nrd2466 · Cited by 2,739 · cited 7×
“This result would be mechanistically similar to the earlier proposed role of S-nitrosothiols (23) or nitrite (10) as stable carriers and transducers of NO-like bioactivity in blood.”
BackgroundResultInfluentialSelf-citationMetabolic Syndrome and Altered Gut Microbiota in Mice Lacking Toll-Like Receptor 5
Vijay-Kumar, M., Aitken, J. D., Carvalho, F. A., Cullender, T. C., Mwangi, S., Srinivasan, S., Sitaraman, S. V., Knight, R., Ley, R. E., & Gewirtz, A. T. (2010) · Science · doi:10.1126/science.1179721 · Cited by 2,068
Nitrite reduction to nitric oxide by deoxyhemoglobin vasodilates the human circulation
Cosby, K., Partovi, K. S., Crawford, J. H., Patel, R. P., Reiter, C. D., Martyr, S., Yang, B. K., Waclawiw, M. A., Zalos, G., Xu, X., et al. (2003) · Nature Medicine · doi:10.1038/nm954 · Cited by 1,807
Kim, J.-A., Montagnani, M., Koh, K. K., & Quon, M. J. (2006) · Circulation · doi:10.1161/circulationaha.105.563213 · Cited by 1,652
S-nitrosohaemoglobin: a dynamic activity of blood involved in vascular control
Jia, L., Bonaventura, C., Bonaventura, J., & Stamler, J. S. (1996) · Nature · doi:10.1038/380221a0 · Cited by 1,592
Flagged first, then by citations
Cited by
Sorted by citations
Items 201–300 of 381
Bradley, J., Islam, K. N., Polhemus, D. J., Donnarumma, E., Brewster, L. P., Tao, Y., Goodchild, T. T., & Lefer, D. J. (2015) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00163.2015 · Cited by 20
Investigation of the nitrate and nitrite contents in milk and milk powder in Taiwan
Yeh, T., Liao, S. F., Kuo, C. Y., & Hwang, W. I. (2013) · Journal of Food and Drug Analysis · doi:10.6227/jfda.2013210109 · Cited by 20
Gheibi, S., Jeddi, S., Carlström, M., Kashfi, K., & Ghasemi, A. (2019) · Nitric Oxide · doi:10.1016/j.niox.2019.08.006 · Cited by 20
Self-citationYang, Z., Du, C., Chang, Z., Yang, Y., & Hu, L. (2025) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2025.1612223 · Cited by 19
Skeletal muscle as a reservoir for nitrate and nitrite: The role of xanthine oxidase reductase (XOR)
de Zevallos, J. O., Woessner, M. N., & Kelley, E. E. (2022) · Nitric Oxide · doi:10.1016/j.niox.2022.10.004 · Cited by 19
Khorasani, V., Jeddi, S., Yaghmaei, P., Tohidi, M., & Ghasemi, A. (2019) · Nitric Oxide · doi:10.1016/j.niox.2019.02.003 · Cited by 19
Bahadoran, Z., Mirmiran, P., Kashfi, K., & Ghasemi, A. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22094735 · Cited by 19
High serum nitric oxide metabolites and incident metabolic syndrome
Ghasemi, A., Zahediasl, S., & Azizi, F. (2012) · Scandinavian Journal of Clinical and Laboratory Investigation · doi:10.3109/00365513.2012.701322 · Cited by 19
Park, J. W., Piknova, B., Walter, P. J., Cai, H., Upanan, S., Thomas, S. M., Tunau-Spencer, K. J., & Schechter, A. N. (2023) · Scientific Reports · doi:10.1038/s41598-023-28190-2 · Cited by 20
Laranjinha, J. A. N., Nunes, C., Ledo, A., Lourenço, C. F., Rocha, B. S., & Barbosa, R. M. (2020) · Neurochemical Research · doi:10.1007/s11064-020-03015-0 · Cited by 18
PPARα-independent effects of nitrate supplementation on skeletal muscle metabolism in hypoxia
O’Brien, K. A., Horscroft, J. A., Devaux, J. B. L., Lindsay, R. T., Steel, A. S., Clark, A. D., Philp, A., Harridge, S. D. R., & Murray, A. J. (2018) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2018.07.027 · Cited by 18
Norouzirad, R., Gholami, H., Ghanbari, M., Hedayati, M., González‐Muniesa, P., Jeddi, S., & Ghasemi, A. (2019) · Life Sciences · doi:10.1016/j.lfs.2019.05.068 · Cited by 18
Zhou, J., Liu, H., Hu, L., Kagami, H., & Wang, S. (2024) · Medicine Plus · doi:10.1016/j.medp.2023.100003 · Cited by 17
Aggarwal, H., Pathak, P., Singh, P., Gayen, J. R., Jagavelu, K., & Dikshit, M. (2020) · Antioxidants · doi:10.3390/antiox9080736 · Cited by 17
Potential Roles of Endoplasmic Reticulum Stress and Cellular Proteins Implicated in Diabesity
Mustapha, S., Mohammed, M., Azemi, A. K., Yunusa, I. U., Shehu, A., Mustapha, L., Wada, Y., Ahmad, M. H., Ahmad, W. A. N. W., Rasool, A. H. G., et al. (2021) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2021/8830880 · Cited by 18
Lewis, S. E., Rosencrance, C. B., DeVallance, E., Giromini, A. P., Williams, X. M., Oh, J., Schmidt, H. M., Straub, A. C., Chantler, P. D., Patel, R. P., et al. (2021) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2021.07.012 · Cited by 19
Effects of long-term oral nitrate administration on adiposity in normal adult female rats
Bakhtiarzadeh, F., Siavoshi, F., Gheibi, S., Kashfi, K., Samadi, R., Jeddi, S., & Ghasemi, A. (2018) · Life Sciences · doi:10.1016/j.lfs.2018.08.032 · Cited by 17
Beneficial effects of inorganic nitrate in non-alcoholic fatty liver disease
Liu, Y., Croft, K. D., Caparros-Martin, J. A., O’Gara, F., Mori, T. A., & Ward, N. C. (2021) · Archives of Biochemistry and Biophysics · doi:10.1016/j.abb.2021.109032 · Cited by 16
Nitric oxide and insulin resistance
(2015) · Immunoendocrinology · doi:10.14800/ie.657 · Cited by 16
Porpino, S. K. P., Zollbrecht, C., Peleli, M., Montenegro, M. F., Brandão, M. C. R., de Athayde-Filho, P. F., França-Silva, M. S., Larsson, E., Lundberg, J. O., Weitzberg, E., et al. (2016) · British Journal of Pharmacology · doi:10.1111/bph.13511 · Cited by 16
Self-citationNaturally cured meats: Quality, safety, and chemistry
Sullivan, G. A. (2011) · Iowa State University Digital Repository (Iowa State University) · doi:10.31274/etd-180810-2479 · Cited by 16
da Silva, D. V. T., Baião, D. D. S., de Almeida, C. C., & Paschoalin, V. M. F. (2023) · Nutrients · doi:10.3390/nu15112618 · Cited by 15
Xu, S., Chuang, C. Y., Malle, E., Gamon, L. F., Hawkins, C. L., & Davies, M. J. (2022) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2022.06.222 · Cited by 15
Yingchoncharoen, T., Rakyhao, T., Chuncharunee, S., Sritara, P., Pienvichit, P., Paiboonsukwong, K., Sathavorasmith, P., Sirirat, K., Sriwantana, T., Srihirun, S., et al. (2017) · Nitric Oxide · doi:10.1016/j.niox.2017.09.010 · Cited by 15
Bahadoran, Z., Jeddi, S., Gheibi, S., Mirmiran, P., Kashfi, K., & Ghasemi, A. (2020) · EXCLI journal · doi:10.17179/excli2020-2515 · Cited by 15
Dietary nitrate and brain health. Too much ado about nothing or a solution for dementia prevention?
Siervo, M., Babateen, A. M., Alharbi, M., Stephan, B. C. M., & Shannon, O. M. (2022) · British Journal Of Nutrition · doi:10.1017/s0007114522002434 · Cited by 14
Aggarwal, H., Pathak, P., Kumar, Y., Jagavelu, K., & Dikshit, M. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms23010195 · Cited by 13
Ezejiofor, A. N., Orish, C. N., & Orisakwe, O. E. (2014) · Chinese Journal of Natural Medicines · doi:10.1016/s1875-5364(14)60114-x · Cited by 13
Bailey, S. J., Blackwell, J. R., Wylie, L. J., Emery, A., Taylor, E., Winyard, P. G., & JONES, A. M. (2016) · Nitric Oxide · doi:10.1016/j.niox.2016.12.008 · Cited by 13
Matthews, V. B., Hollingshead, R. L., Koch, H., Croft, K. D., & Ward, N. C. (2018) · International Journal of Endocrinology · doi:10.1155/2018/7969750 · Cited by 13
Oral nitrite restores age-dependent phenotypes in eNOS-null mice
Τενοπούλου, Μ., Doulias, P., Nakamoto, K., Berríos, K. N., Zura, G., Li, C., Faust, M., Yakovishina, V., Evans, P., Tan, L., et al. (2018) · JCI Insight · doi:10.1172/jci.insight.122156 · Cited by 13
Reciprocal regulation of sulfite oxidation and nitrite reduction by mitochondrial sulfite oxidase
Kaczmarek, A. T., Strampraad, M. J. F., Hagedoorn, P., & Schwarz, G. (2019) · Nitric Oxide · doi:10.1016/j.niox.2019.04.004 · Cited by 13
McNally, B. D., Moran, A., Watt, N. T., Ashmore, T., Whitehead, A., Murfitt, S. A., Kearney, M. T., Cubbon, R. M., Murray, A. J., Griffin, J. L., et al. (2020) · Diabetes · doi:10.2337/db19-0892 · Cited by 13
Yousefzadeh, N., Jeddi, S., Zarkesh, M., Kashfi, K., & Ghasemi, A. (2023) · Scientific Reports · doi:10.1038/s41598-023-31240-4 · Cited by 12
Nitric Oxide Formation From Inorganic Nitrate
Lundberg, J. O., & Weitzberg, E. (2017) · Elsevier eBooks · doi:10.1016/b978-0-12-804273-1.00012-0 · Cited by 13
Self-citationCampelj, D. G., Debruin, D. A., Timpani, C. A., Hayes, A., Goodman, C. A., & Rybalka, E. (2020) · Scientific Reports · doi:10.1038/s41598-020-71974-z · Cited by 12
Oral and systemic diseases under the framework of homeostatic medicine
Zhou, J., Li, X., Hu, L., & Wang, S. (2026) · Dental Research · doi:10.1016/j.dtrs.2026.100027 · Cited by 11
Cytoplasmic Protein Serine/Threonine Kinases
Thiriet, M. (2012) · Biomathematical and biomechanical modeling of the circulatory and ventilatory systems · doi:10.1007/978-1-4614-4370-4_5 · Cited by 11
Zhang, S., Jiang, J., Luo, Y., Liu, G., Hu, S., Wan, S., Luo, C., Li, H., Li, N., Vasconcelos, J. D. G. E. S., et al. (2025) · Frontiers in Cardiovascular Medicine · doi:10.3389/fcvm.2025.1613900 · Cited by 11
El consumo de nitrato y su potencial efecto benéfico sobre la salud cardiovascular
Brayan Moreno, C., Karen Soto, O., & González, D. R. (2015) · Revista chilena de nutrición · doi:10.4067/s0717-75182015000200013 · Cited by 13
The potential use of salivary nitrite as a marker of NO status in humans
Bryan, N. S. (2014) · Nitric Oxide · doi:10.1016/j.niox.2014.12.011 · Cited by 11
Capó, X., Ferrer, M. D., Olek, R. A., Salaberry, E., Suau, R., Marí, B., Llompart, I., Tur, J. A., Sureda, A., & Pons, A. (2020) · Antioxidants · doi:10.3390/antiox9070596 · Cited by 11
Jeddi, S., Gheibi, S., Carlström, M., Kashfi, K., & Ghasemi, A. (2020) · Life Sciences · doi:10.1016/j.lfs.2020.118770 · Cited by 11
Self-citationEvidence-based sports supplements: A redox analysis
Margaritelis, N. V., Cobley, J. N., Nastos, G. G., Παπανικολάου, Κ., Bailey, S. J., Kritsiligkou, P., & Nikolaidis, M. G. (2024) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2024.08.012 · Cited by 10
Nitrate-Reducing Oral Bacteria: Linking Oral and Systemic Health
Bryan, N. S., & Petrosino, J. F. (2017) · Springer eBooks · doi:10.1007/978-3-319-46189-2_3 · Cited by 11
Kang, J., Ha, T. W., Jung, H., Lim, J. E., & Oh, B. (2022) · PLoS ONE · doi:10.1371/journal.pone.0267938 · Cited by 10
Positive feedback loop between dietary nitrate intake and oral health
Zhang, H., & Qin, L. (2023) · Nutrition Research · doi:10.1016/j.nutres.2023.04.008 · Cited by 10
Pharmacological therapies, lifestyle choices and nitric oxide deficiency: A perfect storm
Bryan, N. S. (2012) · Pharmacological Research · doi:10.1016/j.phrs.2012.09.009 · Cited by 10
Nitric oxide is required for the insulin sensitizing effects of contraction in mouse skeletal muscle
Zhang, X., Hiam, D., Hong, Y. H., Zulli, A., Hayes, A., Rattigan, S., & McConell, G. K. (2017) · The Journal of Physiology · doi:10.1113/jp275133 · Cited by 10
Miyamoto, L., Yamane, M., Tomida, Y., Kono, M., Yamaoka, T., Kawasaki, A., Hatano, A., Tsuda, K., Xu, W., Ikeda, Y., et al. (2017) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.b17-00316 · Cited by 10
Metagenomics: Implications in Oral Health and Disease
Nagarajan, M., Prabhu, V. R., & Kamalakkannan, R. (2017) · Elsevier eBooks · doi:10.1016/b978-0-08-102268-9.00009-4 · Cited by 10
Bryan, N. S. (2015) · Elsevier eBooks · doi:10.1016/b978-0-12-384947-2.00484-0 · Cited by 10
Thiriet, M. (2012) · Biomathematical and biomechanical modeling of the circulatory and ventilatory systems · doi:10.1007/978-1-4614-4370-4_2 · Cited by 9
Brain glucose metabolism: Role of nitric oxide
Ghasemi, A., Jeddi, S., & Kashfi, K. (2024) · Biochemical Pharmacology · doi:10.1016/j.bcp.2024.116728 · Cited by 9
History of Nitrite and Nitrate in Food
KEETON, J. T. (2017) · Springer eBooks · doi:10.1007/978-3-319-46189-2_7 · Cited by 9
Carlström, M., Cananau, C., Checa, A., Wide, K., Sartz, L., Svensson, A., Wheelock, C. E., Westphal, S., Békássy, Z. D., Barany, P. F., et al. (2016) · Nitric Oxide · doi:10.1016/j.niox.2016.05.005 · Cited by 9
Self-citationSilveira, R. F., Fernandes, M. H. M. R., Almeida, A. K., de Araújo, R. C., Biagioli, B., Lima, A. R. C., Teixeira, I. A. M. D. A., & de Resende, K. T. (2018) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2018.12.008 · Cited by 9
Fischer, A., Lüersen, K., Schultheiß, G., de Pascual‐Teresa, S., Mereu, A., Ipharraguerre, I. R., & Rimbach, G. H. (2019) · Journal of Clinical Biochemistry and Nutrition · doi:10.3164/jcbn.19-43 · Cited by 9
Inorganic Nitrate Therapy Improves Doxorubicin-Induced Cardiomyopathy
Daiber, A., Gori, T., & Münzel, T. F. (2011) · Journal of the American College of Cardiology · doi:10.1016/j.jacc.2011.02.019 · Cited by 9
Cytoplasmic Protein Tyrosine Kinases
Thiriet, M. (2012) · Biomathematical and biomechanical modeling of the circulatory and ventilatory systems · doi:10.1007/978-1-4614-4370-4_4 · Cited by 8
A Fibre-Optic Platform for Sensing Nitrate Using Conducting Polymers
Shahnia, S., Ebendorff‐Heidepriem, H., Evans, D., & Shahraam Afshar, V. (2020) · Sensors · doi:10.3390/s21010138 · Cited by 8
Dietary Beetroot Juice – Effects in Patients with COPD: A Review
Chen, M., Chang, S., Xu, Y., Parke, H. G., & Liu, J. (2024) · International Journal of COPD · doi:10.2147/copd.s473397 · Cited by 8
Wang, L., Fu, Z., Gao, B., Mo, X., Liang, P., & Huang, J. (2021) · Environmental Science and Pollution Research · doi:10.1007/s11356-021-17423-4 · Cited by 8
New Thoughts in an Old Player: Role of Nitrite in the Treatment of Ischemic Revascularization
Jia, G., & Sowers, J. R. (2013) · Diabetes · doi:10.2337/db13-1530 · Cited by 8
Jeddi, S., Gheibi, S., Afzali, H., Carlström, M., Kashfi, K., & Ghasemi, A. (2022) · Nitric Oxide · doi:10.1016/j.niox.2022.04.004 · Cited by 8
Self-citationGuanosine Triphosphatases and Their Regulators
Thiriet, M. (2012) · Biomathematical and biomechanical modeling of the circulatory and ventilatory systems · doi:10.1007/978-1-4614-4370-4_9 · Cited by 7
Stamm, P., Kalinovic, S., Oelze, M. W., Steven, S., Czarnowski, A., Kvandová, M., Bayer, F., Reinhardt, C., Münzel, T. F., & Daiber, A. (2022) · Biomedicines · doi:10.3390/biomedicines10030730 · Cited by 7
Yousefzadeh, N., Jeddi, S., Zarkesh, M., Norouzirad, R., Kashfi, K., & Ghasemi, A. (2023) · Pharmacological Reports · doi:10.1007/s43440-023-00499-9 · Cited by 7
Xiao, Y., Liu, C., Lei, R., Wang, Z., Wang, X., Tian, H., Xue, B., Zhou, E., Zhang, K., Hu, J., et al. (2023) · Chemosphere · doi:10.1016/j.chemosphere.2023.140243 · Cited by 7
Dietary nitrate struggles in atherosclerosis
Ramms, B., & Gordts, P. L. S. M. (2015) · Atherosclerosis · doi:10.1016/j.atherosclerosis.2015.12.007 · Cited by 7
Watanabe, N. S., & Yamasaki, H. (2017) · Journal of Nutrition & Food Sciences · doi:10.4172/2155-9600.1000572 · Cited by 7
Yousefzadeh, N., Jeddi, S., Shokri, M., Afzali, H., Norouzirad, R., Kashfi, K., & Ghasemi, A. (2021) · Archives of Medical Research · doi:10.1016/j.arcmed.2021.09.007 · Cited by 7
Ma, J., Li, P., Tao, L., Yang, X., Luo, Y., Guo, X., & Gao, B. (2025) · Ecotoxicology and Environmental Safety · doi:10.1016/j.ecoenv.2025.118993 · Cited by 6
Effects of beetroot juice supplementation on vascular functional and structural changes in aged mice
Tawa, M., Nakagawa, K., & Ohkita, M. (2023) · Physiological Reports · doi:10.14814/phy2.15755 · Cited by 6
Gonçalves, J. S., Marçal, A. L., Marques, B. S., Costa, F. D., Laranjinha, J., Rocha, B. S., & Lourenço, C. F. (2024) · Biochemical Society Transactions · doi:10.1042/bst20230491 · Cited by 6
Karimzadeh, L., Behrouz, V., Sohrab, G., Razavion, T., & Haji‐Maghsoudi, S. (2024) · International Journal of Food Sciences and Nutrition · doi:10.1080/09637486.2024.2395817 · Cited by 6
Nitric oxide in modulating oxidative stress mediated skeletal muscle insulin resistance
Anwar, A., Shukla, S., & Pathak, P. (2024) · Molecular Biology Reports · doi:10.1007/s11033-024-09874-y · Cited by 6
The effects of dietary nitrate on plasma glucose and insulin sensitivity in sheep
Villar, M. L., Godwin, I. R., Hegarty, R. S., Dobos, R. C., Smith, K. A., Clay, J. W., & Nolan, J. V. (2019) · Journal of Animal Physiology and Animal Nutrition · doi:10.1111/jpn.13174 · Cited by 6
Impaired Nitric Oxide Metabolism in Type 2 Diabetes: At a Glance
Bahadoran, Z., Carlström, M., Mirmiran, P., & Ghasemi, A. (2022) · BENTHAM SCIENCE PUBLISHERS eBooks · doi:10.2174/9789815079814122010006 · Cited by 6
Self-citationDietary Nitrite: from menace to marvel
Bryan, N. S. (2016) · Functional Foods in Health and Disease · doi:10.31989/ffhd.v6i11.295 · Cited by 6
Liao, G., He, C., Zhang, Y., & Zhang, J. (2025) · Obesity Reviews · doi:10.1111/obr.70021 · Cited by 5
Bahadoran, Z., Mirmiran, P., Ghasemi, A., & Azizi, F. (2024) · Journal of Diabetes & Metabolic Disorders · doi:10.1007/s40200-024-01517-0 · Cited by 5
Liu, Y., Croft, K. D., Mori, T. A., Gaspari, T. A., Kemp‐Harper, B. K., & Ward, N. C. (2023) · European Journal of Nutrition · doi:10.1007/s00394-023-03127-7 · Cited by 5
Oghbaei, H., Alipour, M. R., Mohaddes, G., Hamidian, G., & Keyhanmanesh, . (2019) · Endocrine Regulations · doi:10.2478/enr-2019-0003 · Cited by 5
Cortés‐Puch, I., Sun, J., Schechter, A. N., Solomon, S. B., Park, J. W., Feng, J., Gilliard, C. N., Natanson, C., & Piknova, B. (2019) · Nitric Oxide · doi:10.1016/j.niox.2019.07.001 · Cited by 5
Wynne, A. G., & Affourtit, C. (2022) · PLoS ONE · doi:10.1371/journal.pone.0266905 · Cited by 5
Mitogen-Activated Protein Kinase Module
Thiriet, M. (2012) · Biomathematical and biomechanical modeling of the circulatory and ventilatory systems · doi:10.1007/978-1-4614-4370-4_6 · Cited by 5
Allergic activation of mast cells.
Parker, C. W. (1986) · PubMed · Cited by 5
The hemodynamic response to nitrite is acute and dependent upon tissue perfusion
Dunaway, L. S., Saii, K., LoBue, A., Nyshadham, S., Abib, N. A., Heuser, S. K., Loeb, S. A., Simonsen, U., Cortese-Krott, M. M., & Isakson, B. E. (2024) · Nitric Oxide · doi:10.1016/j.niox.2024.07.005 · Cited by 4
Sowton, A. P., Holzner, L. M. W., Krause, F. N., Baxter, R. M., Mocciaro, G., Krzyzanska, D., Minnion, M., O’Brien, K. A., Harrop, M. C., Darwin, P. M., et al. (2024) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00256.2024 · Cited by 6
Shahraki, Z. S., Karbalaei, N., & Nemati, M. (2020) · Journal of Diabetes & Metabolic Disorders · doi:10.1007/s40200-020-00516-1 · Cited by 4
Zhou, J., Pan, W., Li, X., & Wang, S. (2023) · Chinese Science Bulletin (Chinese Version) · doi:10.1360/tb-2023-0917 · Cited by 4
Protection from diabetic cardiomyopathy — Putative role of the retinoid receptor-mediated signaling
Rassaf, T., & Kelm, M. (2013) · Journal of Molecular and Cellular Cardiology · doi:10.1016/j.yjmcc.2013.03.015 · Cited by 4
Isosorbide-5-mononitrate and endothelial function: a wolf in sheep's clothing
Rassaf, T., & Kelm, M. (2012) · European Heart Journal · doi:10.1093/eurheartj/ehs214 · Cited by 4
Impact of different hormones on the regulation of nitric oxide in diabetes
Gluvic, Z. M., Obradovic, M. M., Manojlovic, M. S., Giglio, R. V., Patti, A. M., Ciaccio, M., Suri, J. S., Rizzo, M., & Isenović, E. R. (2024) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2024.112325 · Cited by 3
Organic vs. inorganic nitrates: Metabolic and vascular outcomes in STZ-induced diabetes in mice
Fernandes-Costa, F., da Silva, R. T. G., de Almeida, A. J. P. O., de Medeiros, I. A., Tafuri, L. S. D. A., dos Santos, G. J., Carlström, M., & Cruz, J. C. (2024) · Life Sciences · doi:10.1016/j.lfs.2024.123257 · Cited by 3
Self-citationNitric Oxide and Type 2 Diabetes: Lessons from Genetic Studies
Bahadoran, Z., Mirmiran, P., Carlström, M., & Ghasemi, A. (2022) · BENTHAM SCIENCE PUBLISHERS eBooks · doi:10.2174/9789815079814122010009 · Cited by 3
Self-citationCarlström, M. (2010) · Current Cardiovascular Risk Reports · doi:10.1007/s12170-010-0139-z · Cited by 3
Self-citationNitrates, nitrites, oxyde nitrique (NO) : nouvelles perspectives pour la santé ?
Bourre, J. M., Buson, C., & L'hirondel, J.-L. (2011) · Médecine et Nutrition · doi:10.1051/mnut/201120001 · Cited by 3
Carlström, M., & Lundberg, J. O. (2016) · Circulation · doi:10.1161/circulationaha.116.021905 · Cited by 3
Self-citation
Metadata
- Authors
- Carlström, M., Larsen, F. J., Nyström, T., Hezel, M., Borniquel, S., Weitzberg, E., & Lundberg, J. O.
- Affiliations
- Uppsala University (SE)
- Karolinska Institutet (SE)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2010-09-27
- Volume
- 107
- Issue
- 41
- Pages
- 17716-17720
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC2955084
- OpenAlex
- W2074172668
- Cited by
- 382
- References
- 43
- Citations by year
- 2026: 9 · 2025: 14 · 2024: 18 · 2023: 21 · 2022: 24 · 2021: 15 · 2020: 31 · 2019: 22 · 2018: 25 · 2017: 33 · 2016: 33 · 2015: 30 · 2014: 31 · 2013: 22 · 2012: 29
- FWCI
- 16.23
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Wenner-Gren Foundation
- European Commission
- Kommunfullmäktige, Stockholms Stad
- Karolinska Institutet
- Vetenskapsrådet
- Svenska Läkaresällskapet
- Topics
- Health Sciences › Medicine › Physiology (medical) › Nitric Oxide and Endothelin Effects
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiovascular Health and Disease Prevention
- Health Sciences › Medicine › Biochemistry (medical) › Phytochemicals and Antioxidant Activities
- Keywords
- dietary nitrate, sodium nitrate, metabolic syndrome, endothelial nitric oxide synthase, nitrate-nitrite-NO pathway, visceral fat accumulation, triglycerides, prediabetes, blood pressure, cardiovascular disease prevention, type 2 diabetes, eNOS-deficient mice
- MeSH terms
- Analysis of Variance, Animals, Blood Pressure, Body Weight, Mice, Mutant Strains, Nitrates, Nitrogen Oxides, Triglycerides, Dietary Supplements, Metabolic Syndrome, Intra-Abdominal Fat, Mice, Rats, Nitric Oxide Synthase Type III
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
A language model drew the claims from the paper on 2026-10-09, under version 3 of the claim definition (model deepseek-v4.1-flash, prompt extract-v3).
A second model labelled each claim on 2026-10-09 (model jev-1.13-20260917, definition revision gold-guideline-v0.2).
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
