The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Genomewide view of gene silencing by small interfering RNAs
Open accessAbstract
RNA interference (RNAi) is an evolutionarily conserved mechanism in plant and animal cells that directs the degradation of messenger RNAs homologous to short double-stranded RNAs termed small interfering RNA (siRNA). The ability of siRNA to direct gene silencing in mammalian cells has raised the possibility that siRNA might be used to investigate gene function in a high throughput fashion or to modulate gene expression in human diseases. The specificity of siRNA-mediated silencing, a critical consideration in these applications, has not been addressed on a genomewide scale. Here we show that siRNA-induced gene silencing of transient or stably expressed mRNA is highly gene-specific and does not produce secondary effects detectable by genomewide expression profiling. A test for transitive RNAi, extension of the RNAi effect to sequences 5′ of the target region that has been observed in Caenorhabditis elegans , was unable to detect this phenomenon in human cells.
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Cites
Cluster analysis and display of genome-wide expression patterns
Eisen, M. B., Spellman, P. T., Brown, P. O., & Botstein, D. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.25.14863 · Cited by 16,184
Self-citationPotent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998) · Nature · doi:10.1038/35888 · Cited by 15,429
Cited by
RNA Interference: Biology, Mechanism, and Applications
Agrawal, N., Dasaradhi, P. V. N., Mohmmed, A., Malhotra, P., Bhatnagar, R. K., & Mukherjee, S. K. (2003) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.67.4.657-685.2003 · Cited by 1,305
Kawahara, T. L. A., Michishita, E., Adler, A. S., Damian, M., Berber, E., Lin, M., McCord, R. A., Ongaigui, K. C. L., Boxer, L. D., Chang, H. Y., et al. (2009) · Cell · doi:10.1016/j.cell.2008.10.052 · Cited by 1,090
Self-citationJackson, A. L., & Linsley, P. S. (2010) · Nature Reviews Drug Discovery · doi:10.1038/nrd3010 · Cited by 1,083
siRNAs: applications in functional genomics and potential as therapeutics
Dorsett, Y., & Tuschl, T. (2004) · Nature Reviews Drug Discovery · doi:10.1038/nrd1345 · Cited by 909
Os8N3 is a host disease-susceptibility gene for bacterial blight of rice
Yang, B., Sugio, A., & White, F. F. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0604088103 · Cited by 639
Metadata
- Authors
- Chi, J.-T., Chang, H. Y., Wang, N. N., Chang, D. S., Dunphy, N., & Brown, P. O.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2003-05-02
- Volume
- 100
- Issue
- 11
- Pages
- 6343-6346
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2082353493
- Cited by
- 285
- References
- 2
- FWCI
- 19.19
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Chi, J.-T., Chang, H. Y., Wang, N. N., Chang, D. S., Dunphy, N., & Brown, P. O. (2003). Genomewide view of gene silencing by small interfering RNAs. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1037853100
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