The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Insights into the oxidative degradation of cellulose by a copper metalloenzyme that exploits biomass components
Open accessAbstract
The enzymatic degradation of recalcitrant plant biomass is one of the key industrial challenges of the 21st century. Accordingly, there is a continuing drive to discover new routes to promote polysaccharide degradation. Perhaps the most promising approach involves the application of “cellulase-enhancing factors,” such as those from the glycoside hydrolase (CAZy) GH61 family. Here we show that GH61 enzymes are a unique family of copper-dependent oxidases. We demonstrate that copper is needed for GH61 maximal activity and that the formation of cellodextrin and oxidized cellodextrin products by GH61 is enhanced in the presence of small molecule redox-active cofactors such as ascorbate and gallate. By using electron paramagnetic resonance spectroscopy and single-crystal X-ray diffraction, the active site of GH61 is revealed to contain a type II copper and, uniquely, a methylated histidine in the copper's coordination sphere, thus providing an innovative paradigm in bioinorganic enzymatic catalysis.
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Emsley, P., & Cowtan, K. (2004) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444904019158 · Cited by 30,571
Cited by
O'Connell, R. J., Thon, M. R., Hacquard, S., Amyotte, S. G., Kleemann, J., Torres, M. F., Damm, U., Buiate, E. A., Epstein, L., Alkan, N., et al. (2012) · Nature Genetics · doi:10.1038/ng.2372 · Cited by 1,047
Payne, C. M., Knott, B. C., Mayes, H. B., Hansson, H., Himmel, M. E., Sandgren, M., Ståhlberg, J., & Beckham, G. T. (2015) · Chemical Reviews · doi:10.1021/cr500351c · Cited by 855
Oxidative cleavage of polysaccharides by monocopper enzymes depends on H2O2
Bissaro, B., Røhr, Å. K., Müller, G., Chylenski, P., Skaugen, M., Forsberg, Z., Horn, S. J., Vaaje-Kolstad, G., & Eijsink, V. G. H. (2017) · Nature Chemical Biology · doi:10.1038/nchembio.2470 · Cited by 594
Shah, F., Nicolás, C., Bentzer, J., Ellström, M., Smits, M., Rineau, F., Canbäck, B., Floudas, D., Carleer, R., Lackner, G., et al. (2016) · New Phytologist · doi:10.1111/nph.13722 · Cited by 394
Lytic Polysaccharide Monooxygenases in Biomass Conversion
Hemsworth, G. R., Johnston, E. M., Davies, G. J., & Walton, P. H. (2015) · Trends in Biotechnology · doi:10.1016/j.tibtech.2015.09.006 · Cited by 314
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Metadata
- Authors
- Quinlan, R. J., Sweeney, M. D., Lo Leggio, L., Otten, H., Poulsen, J.-C. N., Johansen, K. S., Krogh, K. B. R. M., Jørgensen, C. I., Tovborg, M., Anthonsen, A., Tryfona, T., Walter, C. P., Dupree, P., Xu, F., Davies, G. J., & Walton, P. H.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2011-08-29
- Volume
- 108
- Issue
- 37
- Pages
- 15079-15084
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1967796434
- Cited by
- 1,036
- References
- 1
- FWCI
- 37.56
- Type
- journal article
- Language
- en
- Open access
- green
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- Quinlan, R. J., Sweeney, M. D., Lo Leggio, L., Otten, H., Poulsen, J.-C. N., Johansen, K. S., Krogh, K. B. R. M., Jørgensen, C. I., Tovborg, M., Anthonsen, A., Tryfona, T., Walter, C. P., Dupree, P., Xu, F., Davies, G. J., & Walton, P. H. (2011). Insights into the oxidative degradation of cellulose by a copper metalloenzyme that exploits biomass components. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1105776108
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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