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Cited by 411

The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.

Compressive fluorescence microscopy for biological and hyperspectral imaging

Open access
Studer, Vincent, Bobin, Jérome, Chahid, Makhlad, Mousavi, Hamed Shams, Candes, Emmanuel, Dahan, Maxime·2012·Proceedings of the National Academy of Sciences

Abstract

The mathematical theory of compressed sensing (CS) asserts that one can acquire signals from measurements whose rate is much lower than the total bandwidth. Whereas the CS theory is now well developed, challenges concerning hardware implementations of CS-based acquisition devices—especially in optics—have only started being addressed. This paper presents an implementation of compressive sensing in fluorescence microscopy and its applications to biomedical imaging. Our CS microscope combines a dynamic structured wide-field illumination and a fast and sensitive single-point fluorescence detection to enable reconstructions of images of fluorescent beads, cells, and tissues with undersampling ratios (between the number of pixels and number of measurements) up to 32. We further demonstrate a hyperspectral mode and record images with 128 spectral channels and undersampling ratios up to 64, illustrating the potential benefits of CS acquisition for higher-dimensional signals, which typically exhibits extreme redundancy. Altogether, our results emphasize the interest of CS schemes for acquisition at a significantly reduced rate and point to some remaining challenges for CS fluorescence microscopy.

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Metadata

Authors
Studer, V., Bobin, J., Chahid, M., Mousavi, H. S., Candes, E., & Dahan, M.
Venue
Proceedings of the National Academy of Sciences
Published
2012-06-11
Volume
109
Issue
26
Pages
E1679-87
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2114456237
Cited by
411
References
33
FWCI
22.56
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Compressive fluorescence microscopy for biological and hyperspectral imaging. https://app.cochat.ai/papers/10.1073/pnas.1119511109
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Studer, V., Bobin, J., Chahid, M., Mousavi, H. S., Candes, E., & Dahan, M. (2012). Compressive fluorescence microscopy for biological and hyperspectral imaging. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1119511109

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