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Dilatational band formation in bone
Open accessAbstract
Toughening in hierarchically structured materials like bone arises from the arrangement of constituent material elements and their interactions. Unlike microcracking, which entails micrometer-level separation, there is no known evidence of fracture at the level of bone’s nanostructure. Here, we show that the initiation of fracture occurs in bone at the nanometer scale by dilatational bands. Through fatigue and indentation tests and laser confocal, scanning electron, and atomic force microscopies on human and bovine bone specimens, we established that dilatational bands of the order of 100 nm form as ellipsoidal voids in between fused mineral aggregates and two adjacent proteins, osteocalcin (OC) and osteopontin (OPN). Laser microdissection and ELISA of bone microdamage support our claim that OC and OPN colocalize with dilatational bands. Fracture tests on bones from OC and/or OPN knockout mice (OC −/− , OPN −/− , OC-OPN −/−;−/− ) confirm that these two proteins regulate dilatational band formation and bone matrix toughness. On the basis of these observations, we propose molecular deformation and fracture mechanics models, illustrating the role of OC and OPN in dilatational band formation, and predict that the nanometer scale of tissue organization, associated with dilatational bands, affects fracture at higher scales and determines fracture toughness of bone.
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“Microdamage may appear as debonding of the proteinaceous–hydroxyapatite composite (such as debonding of hydroxyapatite aggregates and noncollagenous proteins) or as slippage of the lamellae along one another or along cement lines (6–8).”
From Bone Mechanical Properties in Healthy and Diseased States (2018) · cites this paper as [7]
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Mechanical properties and the hierarchical structure of bone
Rho, J. Y., Kuhn, L. T., & Zioupos, P. (1998) · Medical Engineering & Physics · doi:10.1016/s1350-4533(98)00007-1 · Cited by 2,596
Materials become insensitive to flaws at nanoscale: Lessons from nature
Gao, H., Ji, B., Jäger, I. L., Arzt, E., & Fratzl, P. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0631609100 · Cited by 1,922
Finite-deformation irreversible cohesive elements for three-dimensional crack-propagation analysis
Ortíz, M., & Pandolfi, A. M. (1999) · International Journal for Numerical Methods in Engineering · doi:10.1002/(sici)1097-0207(19990330)44:9 · Cited by 1,431
Structure and mechanical quality of the collagen–mineral nano-composite in bone
Fratzl, P., Gupta, H. S., Paschalis, E. P., & Roschger, P. (2004) · Journal of Materials Chemistry · doi:10.1039/b402005g · Cited by 1,221
Fantner, G. E., Hassenkam, T., Kindt, J. H., Weaver, J. C., Birkedal, H., Pechenik, L., Cutroni, J. A., Cidade, G. A. G., Stucky, G. D., Morse, D. E., et al. (2005) · Nature Materials · doi:10.1038/nmat1428 · Cited by 933
Mechanistic fracture criteria for the failure of human cortical bone
Nalla, R. K., Kinney, J. H., & Ritchie, R. O. (2003) · Nature Materials · doi:10.1038/nmat832 · Cited by 734
Calvert, P. (1999) · Nature · doi:10.1038/20326 · Cited by 597
Li, X., Chang, W.-C., Chao, Y. J., Wang, R., & Chang, M. (2004) · Nano Letters · doi:10.1021/nl049962k · Cited by 592
Bone recognition mechanism of porcine osteocalcin from crystal structure
Hoang, Q. Q., Sicheri, F., Howard, A. J., & Yang, D. S. C. (2003) · Nature · doi:10.1038/nature02079 · Cited by 554
From brittle to ductile fracture of bone
Peterlik, H., Roschger, P., Klaus Klaushofer, A., & Fratzl, P. (2005) · Nature Materials · doi:10.1038/nmat1545 · Cited by 489
Cited by
Sorted by citations
Bone Mechanical Properties in Healthy and Diseased States
Morgan, E. F., Unnikrisnan, G. U., & Hussein, A. I. (2018) · Annual Review of Biomedical Engineering · doi:10.1146/annurev-bioeng-062117-121139 · Cited by 1,026
Structure and mechanics of interfaces in biological materials
Barthelat, F., Zhen, Y., & Buehler, M. J. (2016) · Nature Reviews Materials · doi:10.1038/natrevmats.2016.7 · Cited by 764
New insights into the biology of osteocalcin
Zoch, M. L., Clemens, T. L., & Riddle, R. C. (2015) · Bone · doi:10.1016/j.bone.2015.05.046 · Cited by 612
Hierarchical Structures of Bone and Bioinspired Bone Tissue Engineering
Liu, Y., Luo, D., & Wang, T. (2016) · Small · doi:10.1002/smll.201600626 · Cited by 482
Jepsen, K. J., Silva, M. J., Vashishth, D., Guo, X.-D. E., & van der Meulen, M. C. H. (2015) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.2539 · Cited by 338
Self-citation
Metadata
- Authors
- Poundarik, A. A., Diab, T., Sroga, G. E., Ural, A., Boskey, A. L., Gundberg, C. M., & Vashishth, D.
- Affiliations
- Rensselaer Polytechnic Institute (US)
- Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180;
- Department of Mechanical Engineering, Villanova University, Villanova, PA 19085;
- Hospital for Special Surgery (US)
- Yale University (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2012-11-05
- Volume
- 109
- Issue
- 47
- Pages
- 19178-19183
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC3511118
- OpenAlex
- W2001957914
- Cited by
- 265
- References
- 49
- Citations by year
- 2026: 5 · 2025: 12 · 2024: 11 · 2023: 18 · 2022: 18 · 2021: 20 · 2020: 43 · 2019: 23 · 2018: 27 · 2017: 13 · 2016: 18 · 2015: 30 · 2014: 17 · 2013: 10
- FWCI
- 19.48
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR49635, R01AR038460, AR38460, R01AR041325, R01AR049635)
- National Institute of Dental and Craniofacial Research (R37DE004141, DE04141, R01DE004141)
- Topics
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Bone and Dental Protein Studies
- Physical Sciences › Materials Science › Biomaterials › Calcium Carbonate Crystallization and Inhibition
- Keywords
- osteocalcin, osteopontin, bone matrix, mineral aggregates, protein colocalization, bone microdamage, knockout mice, hierarchy, fatigue (material), indentation, fracture mechanics modeling
- MeSH terms
- Animals, Bone and Bones, Bone Matrix, Cattle, Enzyme-Linked Immunosorbent Assay, Hardness, Humans, Immunohistochemistry, Osteocalcin, Mice, Knockout, Microscopy, Confocal, Microscopy, Atomic Force, Fractures, Bone, Mice, Osteopontin
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