The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
Localized cell death focuses mechanical forces during 3D patterning in a biofilm
Open accessAbstract
From microbial biofilm communities to multicellular organisms, 3D macroscopic structures develop through poorly understood interplay between cellular processes and mechanical forces. Investigating wrinkled biofilms of Bacillus subtilis , we discovered a pattern of localized cell death that spatially focuses mechanical forces, and thereby initiates wrinkle formation. Deletion of genes implicated in biofilm development, together with mathematical modeling, revealed that ECM production underlies the localization of cell death. Simultaneously with cell death, we quantitatively measured mechanical stiffness and movement in WT and mutant biofilms. Results suggest that localized cell death provides an outlet for lateral compressive forces, thereby promoting vertical mechanical buckling, which subsequently leads to wrinkle formation. Guided by these findings, we were able to generate artificial wrinkle patterns within biofilms. Formation of 3D structures facilitated by cell death may underlie self-organization in other developmental systems, and could enable engineering of macroscopic structures from cell populations.
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Cites
Abràmoff, M. D., Magalhães, P. J., & Ram, S. J. (2004) · Utrecht University Repository (Utrecht University) · Cited by 11,821
Flemming, H.-C., & Wingender, J. (2010) · Nature Reviews Microbiology · doi:10.1038/nrmicro2415 · Cited by 10,137
Whitesides, G. M., & Grzybowski, B. (2002) · Science · doi:10.1126/science.1070821 · Cited by 7,439
Geometry and Physics of Wrinkling
Cerda, E., & Mahadevan, L. (2003) · Physical Review Letters · doi:10.1103/physrevlett.90.074302 · Cited by 1,322
Fruiting body formation by Bacillus subtilis
Branda, S. S., González-Pastor, J. E., Ben-Yehuda, S., Losick, R., & Kolter, R. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.191384198 · Cited by 1,129
Self-organization in biological systems
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Mechanical control of tissue and organ development
Mammoto, T., & Ingber, D. E. (2010) · Development · doi:10.1242/dev.024166 · Cited by 850
Amyloid fibers provide structural integrity to Bacillus subtilis biofilms
Romero, D., Aguilar, C., Losick, R., & Kolter, R. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0910560107 · Cited by 792
Cell Death in Pseudomonas aeruginosa Biofilm Development
Webb, J. S., Thompson, L. S., James, S., Charlton, T., Tolker-Nielsen, T., Koch, B., Givskov, M., & Kjelleberg, S. (2003) · Journal of Bacteriology · doi:10.1128/jb.185.15.4585-4592.2003 · Cited by 599
A master regulator for biofilm formation by Bacillus subtilis
Kearns, D. B., Chu, F., Branda, S. S., Kolter, R., & Losick, R. (2005) · Molecular Microbiology · doi:10.1111/j.1365-2958.2004.04440.x · Cited by 597
Cited by
Critical review on biofilm methods
Azeredo, J., Azevedo, N. F., Briandet, R., Cerca, N., Coenye, T., Costa, A. R., Desvaux, M., Di Bonaventura, G., Hébraud, M., Jaglic, Z., et al. (2017) · Critical Reviews in Microbiology · doi:10.1080/1040841x.2016.1208146 · Cited by 1,045
The Mechanical World of Bacteria
Persat, A., Nadell, C. D., Kim, M. K., Ingremeau, F., Siryaporn, A., Drescher, K., Wingreen, N. S., Bassler, B. L., Gitai, Z., & Stone, H. A. (2015) · Cell · doi:10.1016/j.cell.2015.05.005 · Cited by 615
Hobley, L., Harkins, C., MacPhee, C. E., & Stanley-Wall, N. R. (2015) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuv015 · Cited by 581
“…structures at the surface of the biofilm (Branda et al. 2001; Veening, Hamoen and Kuipers 2005), wrinkle and internal channel formation (Wilking et al. 2013), complex colony spreading (Seminara et al. 2012), extreme hydrophobicity (Epstein et al. 2011) and mechanical stiffness (Asally et al. 2012).”
BackgroundBacterial community morphogenesis is intimately linked to the intracellular redox state
Dietrich, L. E. P., Okegbe, C., Price-Whelan, A., Sakhtah, H., Hunter, R. C., & Newman, D. K. (2013) · Journal of Bacteriology · doi:10.1128/jb.02273-12 · Cited by 291
Biofilms deform soft surfaces and disrupt epithelia
Cont, A., Rossy, T., Al-Mayyah, Z., & Persat, A. (2020) · eLife · doi:10.7554/elife.56533 · Cited by 70
Metadata
- Authors
- Asally, M., Kittisopikul, M., Rué, P., Du, Y., Hu, Z., Çağatay, T., Robinson, A. B., Lu, H., Garcia-Ojalvo, J., & Süel, G. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2012-09-24
- Volume
- 109
- Issue
- 46
- Pages
- 18891-18896
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2022288015
- Cited by
- 349
- References
- 37
- FWCI
- 8.19
- Type
- journal article
- Language
- en
- Open access
- green
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- Asally, M., Kittisopikul, M., Rué, P., Du, Y., Hu, Z., Çağatay, T., Robinson, A. B., Lu, H., Garcia-Ojalvo, J., & Süel, G. M. (2012). Localized cell death focuses mechanical forces during 3D patterning in a biofilm. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1212429109
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