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Blast resistance of CC-NB-LRR protein Pb1 is mediated by WRKY45 through protein–protein interaction
Open accessAbstract
Panicle blast 1 ( Pb1 ) is a panicle blast resistance gene derived from the indica rice cultivar “Modan.” Pb1 encodes a coiled-coil–nucleotide-binding site–leucine-rich repeat (CC-NB-LRR) protein and confers durable, broad-spectrum resistance to Magnaporthe oryzae races. Here, we investigated the molecular mechanisms underlying Pb1 -mediated blast resistance. The Pb1 protein interacted with WRKY45, a transcription factor involved in induced resistance via the salicylic acid signaling pathway that is regulated by the ubiquitin proteasome system. Pb1 -mediated panicle blast resistance was largely compromised when WRKY45 was knocked down in a Pb1 -containing rice cultivar. Leaf-blast resistance by Pb1 overexpression ( Pb1 -ox) was also compromised in WRKY45 knockdown /Pb1 -ox rice. Blast infection induced higher accumulation of WRKY45 in Pb1 -ox than in control Nipponbare rice. Overexpression of Pb1-Quad , a coiled-coil domain mutant that had weak interaction with WRKY45, resulted in significantly weaker blast resistance than that of wild-type Pb1 . Overexpression of Pb1 with a nuclear export sequence failed to confer blast resistance to rice. These results suggest that the blast resistance of Pb1 depends on its interaction with WRKY45 in the nucleus. In a transient system using rice protoplasts, coexpression of Pb1 enhanced WRKY45 accumulation and increased WRKY45-dependent transactivation activity, suggesting that protection of WRKY45 from ubiquitin proteasome system degradation is possibly involved in Pb1-dependent blast resistance.
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Shen, Q.-H., Saijo, Y., Mauch, S., Biskup, C., Bieri, S., Keller, B., Seki, H., Ülker, B., Somssich, I. E., & Schulze-Lefert, P. (2007) · Science · doi:10.1126/science.1136372 · Cited by 751
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Le Roux, C., Huet, G., Jauneau, A., Camborde, L., Trémousaygue, D., Kraut, A., Zhou, B., Levaillant, M., Adachi, H., Yoshioka, H., et al. (2015) · Cell · doi:10.1016/j.cell.2015.04.025 · Cited by 489
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Metadata
- Authors
- Inoue, H., Hayashi, N., Matsushita, A., Xinqiong, L., Nakayama, A., Sugano, S., Jiang, C.-J., & Takatsuji, H.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2013-05-21
- Volume
- 110
- Issue
- 23
- Pages
- 9577-9582
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2121429996
- Cited by
- 226
- References
- 1
- FWCI
- 23.94
- Type
- journal article
- Language
- en
- Open access
- green
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- Inoue, H., Hayashi, N., Matsushita, A., Xinqiong, L., Nakayama, A., Sugano, S., Jiang, C.-J., & Takatsuji, H. (2013). Blast resistance of CC-NB-LRR protein Pb1 is mediated by WRKY45 through protein–protein interaction. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1222155110
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