The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.
In vivo two-photon calcium imaging of neuronal networks
Open accessAbstract
Two-photon calcium imaging is a powerful means for monitoring the activity of distinct neurons in brain tissue in vivo . In the mammalian brain, such imaging studies have been restricted largely to calcium recordings from neurons that were individually dye-loaded through microelectrodes. Previous attempts to use membrane-permeant forms of fluorometric calcium indicators to load populations of neurons have yielded satisfactory results only in cell cultures or in slices of immature brain tissue. Here we introduce a versatile approach for loading membrane-permeant fluorescent indicator dyes in large populations of cells. We established a pressure ejection-based local dye delivery protocol that can be used for a large spectrum of membrane-permeant indicator dyes, including calcium green-1 acetoxymethyl (AM) ester, Fura-2 AM, Fluo-4 AM, and Indo-1 AM. We applied this dye-loading protocol successfully in mouse brain tissue at any developmental stage from newborn to adult in vivo and in vitro. In vivo two-photon Ca 2+ recordings, obtained by imaging through the intact skull, indicated that whisker deflection-evoked Ca 2+ transients occur in a subset of layer 2/3 neurons of the barrel cortex. Thus, our results demonstrate the suitability of this technique for real-time analyses of intact neuronal circuits with the resolution of individual cells.
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Verkhratsky, A., & Nedergaard, M. (2018) · Physiological Reviews · doi:10.1152/physrev.00042.2016 · Cited by 1,678
Imaging large-scale neural activity with cellular resolution in awake, mobile mice
Dombeck, D. A., Khabbaz, A. N., Collman, F., Adelman, T. L., & Tank, D. W. (2007) · Neuron · doi:10.1016/j.neuron.2007.08.003 · Cited by 1,099
Clusters of Hyperactive Neurons Near Amyloid Plaques in a Mouse Model of Alzheimer’s Disease
Busche, M. A., Eichhoff, G., Adelsberger, H., Abramowski, D., Wiederhold, K.-H., Haass, C., Staufenbiel, M., Konnerth, A., & Garaschuk, O. (2008) · Science · doi:10.1126/science.1162844 · Cited by 1,125
Self-citationHighly selective receptive fields in mouse visual cortex
Niell, C. M., & Stryker, M. P. (2008) · The Journal of Neuroscience · doi:10.1523/jneurosci.0623-08.2008 · Cited by 1,081
Metadata
- Authors
- Stosiek, C., Garaschuk, O., Holthoff, K., & Konnerth, A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2003-05-30
- Volume
- 100
- Issue
- 12
- Pages
- 7319-7324
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2046031057
- Cited by
- 1,312
- References
- 42
- FWCI
- 17.43
- Type
- journal article
- Language
- en
- Open access
- green
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