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An alternative polyadenylation mechanism coopted to the Arabidopsis RPP7 gene through intronic retrotransposon domestication

Open access
Tsuchiya, Tokuji, Eulgem, Thomas·2013·Proceedings of the National Academy of Sciences

Abstract

Significance: We show the histone mark H3K9me2, which is known to mediate transposon silencing, to determine the choice between alternative polyadenylation sites within the Arabidopsis thaliana disease resistance gene RPP7 . High H3K9me2 levels recruited to the first RPP7 intron by the COPIA-R7 retrotransposon suppress use of a promoter-proximal polyadenylation site. Modulating H3K9me2 levels at this site shifts the balance between full-length and incomplete RPP7 transcripts. By recruiting H3K9me2-dependent polyadenylation control to RPP7 , the COPIA-R7 insertion provided a new switch to fine-tune RPP7 expression. Selective advantages resulting from this mechanism likely contributed to the domestication of COPIA-R7 at RPP7 .

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Metadata

Authors
Tsuchiya, T., & Eulgem, T.
Venue
Proceedings of the National Academy of Sciences
Published
2013-08-12
Volume
110
Issue
37
Pages
E3535-43
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2000365513
Cited by
167
References
1
FWCI
17.20
Type
journal article
Language
en
Open access
green

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Tsuchiya, T., & Eulgem, T. (2013). An alternative polyadenylation mechanism coopted to the Arabidopsis RPP7 gene through intronic retrotransposon domestication. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1312545110

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