Cited by 123RNA and protein synthesis mechanismsBacterial Genetics and BiotechnologyMicrobial Natural Products and Biosynthesis

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Sequence selectivity of macrolide-induced translational attenuation

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Davis, Amber R., Gohara, David W., Yap, Mee-Ngan F.·2014·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.1410356111PMC PMC4217412

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Abstract

Significance: By globally mapping the position of stalled ribosomes in macrolide-treated Staphylococcus aureus , we identified the proteins whose synthesis is affected by this antibiotic. Rather than acting as a nonselective inhibitor, the binding of macrolides to the ribosome tunnel only induces stalling at a few unique sites that are enriched in proline and charged residues. The sequences that flank the stalling sites are highly variable but are essential for efficient stalling. Although it has long been assumed that macrolides inhibit translation after the synthesis of a few residues, we found that stalling could occur at any point during translation. Our results reveal a striking conservation of stalling motifs among all known arrest peptides that strongly suggests a universal ribosome stalling mechanism.

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Authors: Davis, A. R., Gohara, D. W., & Yap, M.-N. F.
Affiliations: Saint Louis University (US)
ORCID: Gohara, David W.
Yap, Mee-Ngan F.
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 2014-10-13
Volume: 111
Issue: 43
Pages: 15379-15384
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.1410356111
PMC: PMC4217412
OpenAlex: W2165035045
Cited by: 123
References: 46
Citations by year: 2026: 13 · 2025: 10 · 2024: 10 · 2023: 6 · 2022: 8 · 2021: 14 · 2020: 12 · 2019: 8 · 2018: 11 · 2017: 18 · 2016: 10 · 2015: 3
FWCI: 4.23
Type: journal article
Language: en
Open access: bronze
doi.org
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: Saint Louis University
National Institutes of Health
National Institute of General Medical Sciences (R00GM094212)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Microbial Natural Products and Biosynthesis
Keywords: macrolide antibiotics, azithromycin, translational attenuation, ribosome stalling, Staphylococcus aureus, proline-rich sequences, charged residues, translational arrest
MeSH terms: Peptides, Proline, Ribosomes, Staphylococcus aureus, Protein Biosynthesis, Open Reading Frames, Azithromycin, Macrolides, Sequence Analysis, Protein

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Sequence selectivity of macrolide-induced translational attenuation

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