Cited by 123RNA and protein synthesis mechanismsBacterial Genetics and BiotechnologyMicrobial Natural Products and Biosynthesis

The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.

Sequence selectivity of macrolide-induced translational attenuation

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Davis, Amber R., Gohara, David W., Yap, Mee-Ngan F.·2014·Proceedings of the National Academy of Sciences

Abstract

Significance: By globally mapping the position of stalled ribosomes in macrolide-treated Staphylococcus aureus , we identified the proteins whose synthesis is affected by this antibiotic. Rather than acting as a nonselective inhibitor, the binding of macrolides to the ribosome tunnel only induces stalling at a few unique sites that are enriched in proline and charged residues. The sequences that flank the stalling sites are highly variable but are essential for efficient stalling. Although it has long been assumed that macrolides inhibit translation after the synthesis of a few residues, we found that stalling could occur at any point during translation. Our results reveal a striking conservation of stalling motifs among all known arrest peptides that strongly suggests a universal ribosome stalling mechanism.

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Metadata

Authors
Davis, A. R., Gohara, D. W., & Yap, M.-N. F.
Affiliations
Saint Louis University (US)
Venue
Proceedings of the National Academy of Sciences
Published
2014-10-13
Volume
111
Issue
43
Pages
15379-15384
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2165035045
Cited by
123
References
46
Citations by year
2026: 13 · 2025: 10 · 2024: 10 · 2023: 6 · 2022: 8 · 2021: 14 · 2020: 12 · 2019: 8 · 2018: 11 · 2017: 18 · 2016: 10 · 2015: 3
FWCI
4.23
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Saint Louis University
  • National Institutes of Health
  • National Institute of General Medical Sciences (R00GM094212)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Microbial Natural Products and Biosynthesis
Keywords
macrolide antibiotics, azithromycin, translational attenuation, ribosome stalling, Staphylococcus aureus, proline-rich sequences, charged residues, translational arrest
MeSH terms
Peptides, Proline, Ribosomes, Staphylococcus aureus, Protein Biosynthesis, Open Reading Frames, Azithromycin, Macrolides, Sequence Analysis, Protein

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