Goniometer-based femtosecond crystallography with X-ray free electron lasers
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Significance: The extremely short and bright X-ray pulses produced by X-ray free-electron lasers unlock new opportunities in crystallography-based structural biology research. Efficient methods to deliver crystalline material are necessary due to damage or destruction of the crystal by the X-ray pulse. Crystals for the first experiments were 5 µm or smaller in size, delivered by a liquid injector. We describe a highly automated goniometer-based approach, compatible with crystals of larger and varied sizes, and accessible at cryogenic or ambient temperatures. These methods, coupled with improvements in data-processing algorithms, have resulted in high-resolution structures, unadulterated by the effects of radiation exposure, from only 100 to 1,000 diffraction images.
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Similar improvements in limiting resolution of XFEL versus synchrotron diffraction images have been observed for GPCRs.
“We note that similar improvements in limiting resolution of XFEL versus synchrotron diffraction images have been observed for GPCRs60.”
From Architecture of the synaptotagmin–SNARE machinery for neuronal exocytosis (2015) · cites this paper as [60]
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Cited by
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Architecture of the synaptotagmin–SNARE machinery for neuronal exocytosis
Zhou, Q., Lai, Y., Bacaj, T., Zhao, M., Lyubimov, A. Y., Uervirojnangkoorn, M., Zeldin, O. B., Brewster, A. S., Sauter, N. K., Cohen, A. E., et al. (2015) · Nature · doi:10.1038/nature14975 · Cited by 348 · see citing works
Self-citation
- Authors
- Cohen, A. E., Soltis, S. M., González, A., Aguila, L., Alonso-Mori, R., Barnes, C. O., Baxter, E. L., Brehmer, W., Brewster, A. S., Brunger, A. T., Calero, G., Chang, J. F., Chollet, M., Ehrensberger, P., Eriksson, T. L., Feng, Y., Hattne, J., Hedman, B., Hollenbeck, M., Holton, J. M., Keable, S., Kobilka, B. K., Kovaleva, E. G., Kruse, A. C., Lemke, H. T., Lin, G., Lyubimov, A. Y., Manglik, A., Mathews, I. I., McPhillips, S. E., Nelson, S., Peters, J. W., Sauter, N. K., Smith, C. A., Song, J., Stevenson, H. P., Tsai, Y., Uervirojnangkoorn, M., Vinetsky, V., Wakatsuki, S., Weis, W. I., Zadvornyy, O. A., Zeldin, O. B., Zhu, D., & Hodgson, K. O.
- Affiliations
- Stanford Synchrotron Radiation Lightsource (US)
- Stanford Synchrotron Radiation Lightsource,
- Linac Coherent Light Source, and
- University of Pittsburgh (US)
- Lawrence Berkeley National Laboratory (US)
- Molecular and Cellular Physiology, and
- Linac Coherent Light Source (US)
- Montana State University (US)
- Howard Hughes Medical Institute (US)
- Photon Science, SLAC National Accelerator Laboratory, Stanford University, Menlo Park, CA 94025;
- ORCID
- Cohen, Aina E.
- Soltis, S. Michael
- Barnes, Christopher O.
- Brewster, Aaron S.
- Calero, Guillermo
- Chollet, Matthieu
- Eriksson, Thomas L.
- Hattne, Johan
- Holton, James M.
- Kobilka, Brian K.
- Kovaleva, Elena G.
- Kruse, Andrew C.
- Lemke, Henrik T.
- Lin, Guowu
- Lyubimov, Artem Y.
- Manglik, Aashish
- Mathews, Irimpan I.
- Nelson, Silke
- Peters, John W.
- Sauter, Nicholas K.
- Smith, Clyde A.
- Tsai, Yingssu
- Weis, William I.
- Zadvornyy, Oleg A.
- Zhu, Diling
- Hodgson, Keith O.
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2014-10-31
- Volume
- 111
- Issue
- 48
- Pages
- 17122-17127
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC4260607
- OpenAlex
- W2073679797
- Cited by
- 144
- References
- 0
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 3 · 2023: 5 · 2022: 6 · 2021: 8 · 2020: 12 · 2019: 18 · 2018: 15 · 2017: 19 · 2016: 20 · 2015: 28 · 2014: 1
- FWCI
- 10.29
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- Funders
- NIH (P41GM103393, GM102520, GM095887, GM073210, GM082250, GM094625)
- HHMI (HHMI Collaborative Innovation Award (HCIA))
- Topics
- Physical Sciences › Materials Science › Materials Chemistry › Enzyme Structure and Function
- Physical Sciences › Physics and Astronomy › Radiation › Advanced X-ray Imaging Techniques
- Physical Sciences › Materials Science › Materials Chemistry › X-ray Diffraction in Crystallography
- Keywords
- X-ray free-electron laser, metalloenzymes, hydrogenase, myoglobin, beta2-adrenergic receptor, RNA polymerase II, Linac Coherent Light Source, electron density mapping
- MeSH terms
- Chemistry, Physical, Crystallization, Electrons, Lasers, Models, Molecular, Myoglobin, Protein Conformation, Proteins, RNA Polymerase II, X-Ray Diffraction, X-Rays, Reproducibility of Results, Synchrotrons, Receptors, Adrenergic, beta-2, Crystallography, X-Ray
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