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Pel is a cationic exopolysaccharide that cross-links extracellular DNA in the Pseudomonas aeruginosa biofilm matrix
Open accessAbstract
Significance: Exopolysaccharides and extracellular DNA are important structural components that contribute to the self-assembly of large aggregates or microcolonies that are characteristic of biofilms. Pseudomonas aeruginosa is capable of producing multiple exopolysaccharides, including alginate, Psl, and Pel. At present, little is known about Pel’s chemical structure and its role in microcolony formation. Our results demonstrate that Pel is composed of cationic amino sugars. Using this knowledge, we have developed a Pel-specific lectin stain to directly visualize Pel in biofilms. We show that the positive charge on Pel facilitates its binding to extracellular DNA in the biofilm stalk, and that Pel can compensate for lack of Psl in the biofilm periphery.
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Gunn, J. S., Bakaletz, L. O., & Wozniak, D. J. (2016) · Journal of Biological Chemistry · doi:10.1074/jbc.r115.707547 · Cited by 211
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A Critical Role for Extracellular DNA in Dental Plaque Formation
Rostami, N., Shields, R. C., Yassin, S. A., Hawkins, A. R., Bowen, L., Luo, T. L., Rickard, A. H., Holliday, R., Preshaw, P. M., & Jakubovics, N. S. (2017) · Journal of Dental Research · doi:10.1177/0022034516675849 · Cited by 56
Qiu, D., Zheng, C., Zeng, Y., Wu, L., Huang, C., Ran, Y., Ding, Y., Shi, J., Cai, X., & Pan, Y. (2023) · Chemical Engineering Journal · doi:10.1016/j.cej.2023.147217 · Cited by 40
Metadata
- Authors
- Jennings, L. K., Storek, K. M., Ledvina, H. E., Coulon, C., Marmont, L. S., Sadovskaya, I., Secor, P. R., Tseng, B. S., Scian, M., Filloux, A., Wozniak, D. J., Howell, P. L., & Parsek, M. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2015-08-26
- Volume
- 112
- Issue
- 36
- Pages
- 11353-11358
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2124439712
- Cited by
- 624
- References
- 0
- FWCI
- 14.80
- Type
- journal article
- Language
- en
- Open access
- green
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