Cited by 211Bacterial biofilms and quorum sensingEscherichia coli research studiesBacterial Genetics and Biotechnology

What's on the Outside Matters: The Role of the Extracellular Polymeric Substance of Gram-negative Biofilms in Evading Host Immunity and as a Target for Therapeutic Intervention

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Gunn, John S., Bakaletz, Lauren O., Wozniak, Daniel J.·2016·Journal of Biological Chemistry

Abstract

Biofilms are organized multicellular communities encased in an extracellular polymeric substance (EPS). Biofilm-resident bacteria resist immunity and antimicrobials. The EPS provides structural stability and presents a barrier; however, a complete understanding of how EPS structure relates to biological function is lacking. This review focuses on the EPS of three Gram-negative pathogens: Pseudomonas aeruginosa, nontypeable Haemophilus influenzae, and Salmonella enterica serovar Typhi/Typhimurium. Although EPS proteins and polysaccharides are diverse, common constituents include extracellular DNA, DNABII (DNA binding and bending) proteins, pili, flagella, and outer membrane vesicles. The EPS biochemistry promotes recalcitrance and informs the design of therapies to reduce or eliminate biofilm burden.

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Crossref · 10.1074/jbc.r115.707547

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  • New roles are emerging for glycans in the biofilms surrounding bacteria, enabling them to form discrete multicellular communities.

    “and new roles are emerging for glycans in the bio fi lms surrounding bacteria, enabling them to form discrete multicellular communities (579 -583).”

    From Biological roles of glycans (2017) · cites this paper as [583]

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Authors
Gunn, J. S., Bakaletz, L. O., & Wozniak, D. J.
Affiliations
Nationwide Children's Hospital (US)
Corresponding author
Wozniak, Daniel J.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2016-06
Volume
291
Issue
24
Pages
12538-12546
ISSN
0021-9258 (ISSN-L)
OpenAlex
W2338854241
Cited by
211
References
105
Citations by year
2026: 23 · 2025: 30 · 2024: 20 · 2023: 16 · 2022: 20 · 2021: 32 · 2020: 24 · 2019: 18 · 2018: 13 · 2017: 8 · 2016: 7
FWCI
1.38
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of Allergy and Infectious Diseases (R01AI097511, R01AI116917)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
extracellular polymeric substances, biofilm, Gram-negative bacteria, host immune evasion, antimicrobial resistance, Pseudomonas aeruginosa, nontypeable Haemophilus influenzae, Salmonella typhi, extracellular DNA, fimbriae, flagella, outer membrane vesicles, biofilm-targeted therapy
MeSH terms
Anti-Bacterial Agents, Bacterial Infections, DNA, Bacterial, Extracellular Matrix, Gram-Negative Bacteria, Humans, Polysaccharides, Bacterial, Biofilms, Host-Pathogen Interactions, Immune Evasion

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