The DOI matches its Crossref record; 52 of the 52 references it cites were found in a registry or an index.
Self-reproducing catalyst drives repeated phospholipid synthesis and membrane growth
Open accessAbstract
Significance: We report on the design and synthesis of an artificial cell membrane that sustains continual growth. Lipid membranes are ubiquitous in all domains of life. Numerous studies have exploited the ability of lipids to self-assemble into bilayer vesicles with properties reminiscent of cellular membranes, but previous work has yet to mimic nature’s ability to support persistent phospholipid membrane formation. In this work, we have developed an artificial cell membrane that continually synthesizes all of the components needed to form additional catalytic membranes. These results demonstrate that complex lipid membranes capable of indefinite self-synthesis can emerge when supplied with simpler chemical building blocks.
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In the citing paper
“The model lipid catalyst has a headgroup constituting a hydrophilic chelator for Cu1+ ions and the hydrophobic moieties were three modified fatty acid chains. This amphiphilic molecule served as effective catalyst for the covalent bond formation between an alkyne-derivatized lysophospholipid and an azide-derivatized fatty acid analogue, leading to the formation of two-chain phospholipid analogue.”
From Systems protobiology: origin of life in lipid catalytic networks (2018) · cites this paper as [193]
In the citing paper
“The lipid-embodied network has four food compounds, three of which generate a lipid catalyst in two consecutive reactions.”
From Systems protobiology: origin of life in lipid catalytic networks (2018) · cites this paper as [193]
In the citing paper
“The experimental results provided are describable by a simple mutually catalytic network in line with the Kauffman–Hordijk model, as well as by the formalisms of M-GARD (previous section).”
From Systems protobiology: origin of life in lipid catalytic networks (2018) · cites this paper as [193]
In the citing paper
“The catalyst then enhances the rate of its own formation, as well as that of a reaction generating a two-chain phospholipid from a single chain phospholipid and a fatty acid analogue.”
From Systems protobiology: origin of life in lipid catalytic networks (2018) · cites this paper as [193]
In the citing paper
“but highlight a first published experimental instance of a simple lipid-based GARD network [193].”
From Systems protobiology: origin of life in lipid catalytic networks (2018) · cites this paper as [193]
In the citing paper
“Regarding evidence for our model's validity, we point out the insurmountable hurdles for comprehensive experimental verification, but highlight a first published experimental instance of a simple lipid-based GARD network [193].”
From Systems protobiology: origin of life in lipid catalytic networks (2018) · cites this paper as [193]
Citations
Cites
Cu-Catalyzed Azide−Alkyne Cycloaddition
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“The lack of a strong pH effect on the permeability coefficients of measured charged species supports a transient defect mechanism of membrane permeation over a solubility-diffusion mechanism (32).”
BackgroundLiposomes from ionic, single-chain amphiphiles
Hargreaves, W. R., & Deamer, D. W. (1978) · Biochemistry · doi:10.1021/bi00611a014 · Cited by 576
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Cited by
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Liposomes and polymersomes: a comparative review towards cell mimicking
Rideau, E., Dimova, R., Schwille, P., Wurm, F. R., & Landfester, K. (2018) · Chemical Society Reviews · doi:10.1039/c8cs00162f · Cited by 1,144
Artificial Cells: Synthetic Compartments with Life-like Functionality and Adaptivity
Buddingh’, B. C., & van Hest, J. C. M. (2017) · Accounts of Chemical Research · doi:10.1021/acs.accounts.6b00512 · Cited by 684
From dynamic self-assembly to networked chemical systems
Grzybowski, B. A., Fitzner, K., Paczesny, J., & Granick, S. (2017) · Chemical Society Reviews · doi:10.1039/c7cs00089h · Cited by 306
Synthetic Cells: From Simple Bio‐Inspired Modules to Sophisticated Integrated Systems
Guindani, C., da Silva, L. C., Cao, S., Ivanov, T., & Landfester, K. (2021) · Angewandte Chemie International Edition · doi:10.1002/anie.202110855 · Cited by 215
Artificial Cells: Past, Present and Future
Jiang, W., Wu, Z., Gao, Z., Wan, M., Zhou, M., Mao, C., & Shen, J. (2022) · ACS Nano · doi:10.1021/acsnano.2c06104 · Cited by 215
Metadata
- Authors
- Hardy, M. D., Yang, J., Selimkhanov, J., Cole, C. M., Tsimring, L. S., & Devaraj, N. K.
- Affiliations
- University of California San Diego (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2015-06-22
- Volume
- 112
- Issue
- 27
- Pages
- 8187-8192
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC4500204
- OpenAlex
- W1952600287
- Cited by
- 181
- References
- 52
- Citations by year
- 2026: 15 · 2025: 13 · 2024: 13 · 2023: 17 · 2022: 12 · 2021: 17 · 2020: 20 · 2019: 26 · 2018: 16 · 2017: 20 · 2016: 6 · 2015: 6
- FWCI
- 6.95
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Department of Defense (W911NF-13-1-0383)
- National Science Foundation (CHE-1254611)
- HHS | National Institutes of Health (P50GM085764)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
- Physical Sciences › Physics and Astronomy › Astronomy and Astrophysics › Origins and Evolution of Life
- Physical Sciences › Chemistry › Organic Chemistry › Advanced Polymer Synthesis and Characterization
- Keywords
- autocatalysis, phospholipid synthesis, model membranes, membrane growth, vesicle growth, membrane remodeling, biomimetic membranes, lipid bilayer formation, origin of life
- MeSH terms
- Catalysis, Cell Membrane, Copper, Lipid Bilayers, Membrane Lipids, Membranes, Artificial, Models, Chemical, Magnetic Resonance Spectroscopy, Phosphatidylcholines, Phospholipids, Triazoles, Molecular Structure, Microscopy, Confocal, Microscopy, Electron, Transmission, Unilamellar Liposomes, Time-Lapse Imaging, Cycloaddition Reaction
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