Defective histone supply causes changes in RNA polymerase II elongation rate and cotranscriptional pre-mRNA splicing
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Significance: The study of the role of histones in transcription in mammals has been hindered by the existence of large gene families encoding every histone subunit. Here we reduce the level of canonical histones in a human cell line by silencing stem-loop–binding protein, a histone mRNA regulatory factor. We show that canonical histone depletion increases the RNA polymerase II (RNAPII) elongation rate and temporally separates transcription and splicing. Furthermore, histone depletion also causes several pre-mRNA splicing defects, including skipping of alternative exons and intron retention. Thus we demonstrate that the correct histone supply is required to control the RNAPII elongation rate and pre-mRNA splicing.
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Histone depletion from human cells increases transcription speed.
“Consistent with the idea that nucleosomes represent a transcriptional barrier, histone depletion from human cells increases transcription speed (Jimeno‐González et al, 2015).”
From RNA polymerase II speed: a key player in controlling and adapting transcriptome composition (2021) · cites this paper as Jimeno‐González et al, 2015
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RNA polymerase II speed: a key player in controlling and adapting transcriptome composition
Muniz, L., Nicolas, E., & Trouche, D. (2021) · The EMBO Journal · doi:10.15252/embj.2020105740 · Cited by 203 · see citing works
“:3) alez et al , 2015).”
Pause & go: from the discovery of RNA polymerase pausing to its functional implications
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- Authors
- Jimeno-González, S., Payán-Bravo, L., Muñoz-Cabello, A. M., Guijo, M., Gutierrez, G., Prado, F., & Reyes, J. C.
- Affiliations
- Consejo Superior de Investigaciones Científicas (ES)
- Department of Genetics, University of Seville, 41080, Seville, Spain
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2015-11-17
- Volume
- 112
- Issue
- 48
- Pages
- 14840-14845
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC4672771
- OpenAlex
- W2176036947
- Cited by
- 80
- References
- 8
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 9 · 2023: 10 · 2022: 7 · 2021: 9 · 2020: 7 · 2019: 8 · 2018: 5 · 2017: 10 · 2016: 6
- FWCI
- 3.04
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
- Funders
- Ministerio de Economía y Competitividad (BFU2014-53543-P, BFU-2011-23442)
- Andalussian Government (P12CT52270)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Keywords
- RNA polymerase II elongation, histone depletion, chromatin structure, H2AX, H2A.Z, histone H3.3, histone variants, intron retention, alternative splicing, CD44
- MeSH terms
- Histones, Humans, RNA Polymerase II, RNA Precursors, RNA Splicing, Hyaluronan Receptors, Cell Line, Tumor, Transcription Elongation, Genetic
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