The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Conserved interdomain linker promotes phase separation of the multivalent adaptor protein Nck
Open accessAbstract
Significance: Many eukaryotic proteins are composed of tandem arrays of modular domains, which bind peptide ligands that also often appear in tandem arrays. The interdomain linkers in such systems are often considered merely passive elements that flexibly connect the functional domains. Collective interactions among multivalent molecules lead to micron-sized phase-separated states that are thought to be important in cellular organization. Here, we show that in the multivalent signaling adaptor protein Nck, weak interactions involving an interdomain linker play a significant role in self-assembly and phase separation with ligands neuronal Wiskott-Aldrich syndrome protein (N-WASP) and phosphorylated nephrin. Our results suggest that interactions mediated by ordered modular domains may generally act synergistically with weak interactions of disordered interdomain linkers to promote phase separation.
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Kato, M., Han, T. W., Xie, S., Shi, K., Du, X., Wu, L. C., Mirzaei, H., Goldsmith, E. J., Longgood, J., Pei, J., et al. (2012) · Cell · doi:10.1016/j.cell.2012.04.017 · Cited by 2,012
Nott, T. J., Petsalaki, E., Farber, P., Jervis, D., Fussner, E., Plochowietz, A., Craggs, T. D., Bazett-Jones, D. P., Pawson, T., Forman-Kay, J. D., et al. (2015) · Molecular Cell · doi:10.1016/j.molcel.2015.01.013 · Cited by 1,803
Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck
Eden, S., Rohatgi, R., Podtelejnikov, A. V., Mann, M., & Kirschner, M. W. (2002) · Nature · doi:10.1038/nature00859 · Cited by 835
Cited by
A Phase Separation Model for Transcriptional Control
Hnisz, D., Shrinivas, K., Young, R. A., Chakraborty, A. K., & Sharp, P. A. (2017) · Cell · doi:10.1016/j.cell.2017.02.007 · Cited by 1,753
Compositional Control of Phase-Separated Cellular Bodies
Banani, S. F., Rice, A. M., Peeples, W. B., Lin, Y., Jain, S., Parker, R., & Rosen, M. K. (2016) · Cell · doi:10.1016/j.cell.2016.06.010 · Cited by 1,246
Self-citationHarmon, T. S., Holehouse, A. S., Rosen, M. K., & Pappu, R. V. (2017) · eLife · doi:10.7554/elife.30294 · Cited by 713
Self-citationOpenCell: Endogenous tagging for the cartography of human cellular organization
Cho, N. H., Cheveralls, K. C., Brunner, A.-D., Kim, K., Michaelis, A. C., Raghavan, P., Kobayashi, H., Savy, L., Li, J. Y., Canaj, H., et al. (2022) · Science · doi:10.1126/science.abi6983 · Cited by 567
“…compartmentsforwhichbiomolecularconden-sationhasbeenproposedtoplayanimportant role(Fig.5G),namelyP-bodies( 64 ), stress granules ( 59 ), centrosome ( 65 ), cell junctions ( 66 ), and the interface between cell surface and actin cytoskeleton ( 67 ).”
BackgroundBrady, J. P., Farber, P. J., Sekhar, A., Lin, Y.-H., Huang, R., Bah, A., Nott, T. J., Chan, H. S., Baldwin, A. J., Forman-Kay, J. D., et al. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1706197114 · Cited by 533
Metadata
- Authors
- Banjade, S., Wu, Q., Mittal, A., Peeples, W. B., Pappu, R. V., & Rosen, M. K.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2015-11-09
- Volume
- 112
- Issue
- 47
- Pages
- E6426-35
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2125272201
- Cited by
- 183
- References
- 3
- FWCI
- 4.63
- Type
- journal article
- Language
- en
- Open access
- green
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- Banjade, S., Wu, Q., Mittal, A., Peeples, W. B., Pappu, R. V., & Rosen, M. K. (2015). Conserved interdomain linker promotes phase separation of the multivalent adaptor protein Nck. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1508778112
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