The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Cross-strand binding of TFAM to a single mtDNA molecule forms the mitochondrial nucleoid
Open accessAbstract
Significance: Altered expression of mitochondrial DNA (mtDNA) is heavily implicated in human disease and aging, but the basic organizational unit of mtDNA, the mitochondrial nucleoid, is poorly understood. Here, we have used a combination of biochemistry, superresolution microscopy, and electron microscopy to show that mammalian mitochondrial nucleoids have an irregular ellipsoidal shape and typically contain a single copy of mtDNA. Furthermore, we show that the nucleoid ultrastructure is independent of cellular mtDNA copy number and that the core nucleoid structure is formed by cross-strand binding of mitochondrial transcription factor A (TFAM) to a single copy of mtDNA. The clarification of the ultrastructure of the mammalian mitochondrial nucleoid provides the fundamental basis for the understanding of regulation of mtDNA maintenance and expression in mammals.
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Cites
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Mitochondrial transcription factor A is necessary for mtDNA maintance and embryogenesis in mice
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Self-citationMitochondrial transcription factor A regulates mtDNA copy number in mammals
Ekstrand, M. I. (2004) · Human Molecular Genetics · doi:10.1093/hmg/ddh109 · Cited by 919 · cited 3×
“To study the effect of mtDNA copy number on the abundance and morphology of mitochondrial nucleoids in vivo, we generated primary mouse embryonic fibroblast (MEF) cell lines from mice expressing human TFAM (11), herein referred to as “TFAM overexpressor (OE)” MEFs (Fig.”
BackgroundINTRAMITOCHONDRIAL FIBERS WITH DNA CHARACTERISTICS
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Mitochondrial transcription factors B1 and B2 activate transcription of human mtDNA
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“It specifically binds the mitochondrial promoters to recruit the mitochondrial RNA polymerase (POLRMT), which, in turn, recruits mitochondrial transcription factor B2 (14, 15).”
BackgroundMethodSelf-citationKukat, C., Wurm, C. A., Spåhr, H., Falkenberg, M., Larsson, N.-G., & Jakobs, S. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1109263108 · Cited by 563
Self-citationSomatic Mitochondrial DNA Mutations in Mammalian Aging
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Cited by
Mitochondrial proteins: from biogenesis to functional networks
Pfanner, N., Warscheid, B., & Wiedemann, N. (2019) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-018-0092-0 · Cited by 1,028
Fluorescence nanoscopy in cell biology
Sahl, S. J., Hell, S. W., & Jakobs, S. (2017) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.71 · Cited by 990
Self-citationZhao, M., Wang, Y., Li, L., Liu, S., Wang, C., Yuan, Y., Yang, G., Chen, Y., Cheng, J., Lu, Y., et al. (2021) · Theranostics · doi:10.7150/thno.50905 · Cited by 820
Structure and function of mitochondrial membrane protein complexes
Kühlbrandt, W. (2015) · BMC Biology · doi:10.1186/s12915-015-0201-x · Cited by 699
Self-citationER-mitochondria contacts couple mtDNA synthesis with mitochondrial division in human cells
Lewis, S. C., Uchiyama, L. F., & Nunnari, J. (2016) · Science · doi:10.1126/science.aaf5549 · Cited by 673
Metadata
- Authors
- Kukat, C., Davies, K. M., Wurm, C. A., Spåhr, H., Bonekamp, N. A., Kühl, I., Joos, F., Polosa, P. L., Park, C. B., Posse, V., Falkenberg, M., Jakobs, S., Kühlbrandt, W., & Larsson, N.-G.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2015-08-24
- Volume
- 112
- Issue
- 36
- Pages
- 11288-11293
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2162006879
- Cited by
- 375
- References
- 39
- FWCI
- 11.06
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Kukat, C., Davies, K. M., Wurm, C. A., Spåhr, H., Bonekamp, N. A., Kühl, I., Joos, F., Polosa, P. L., Park, C. B., Posse, V., Falkenberg, M., Jakobs, S., Kühlbrandt, W., & Larsson, N.-G. (2015). Cross-strand binding of TFAM to a single mtDNA molecule forms the mitochondrial nucleoid. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1512131112
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