Nascent chain-monitored remodeling of the Sec machinery for salinity adaptation of marine bacteria.
Open accessAbstract
Significance: Bacteria living in seawater must cope with low-sodium environments that they may encounter. Here we show an unexpected finding that remodeling of the Sec protein export machinery plays a pivotal role in this adaptation. Vibrio alginolyticus possesses alternative SecDF1 and SecDF2 homologs that use the transmembrane gradient of Na + and that of H + , respectively, to enhance protein export by cooperating with the SecYEG translocon. The synthesis of SecDF2 is induced in low-Na + environments, and this induction is essential for the bacterium to survive low salinity. Remarkably, the Vibrio species use a nascent polypeptide, VemP, to monitor the functional state of the Sec pathway and to up-regulate translation of SecDF2 when activity of the SecDF1-containing Sec machinery declines.
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In Vibrio alginolyticus, the substrate VemP controls the switch between a sodium-coupled SecDF2 complex and a proton-coupled SecDF1 complex in low Na+ environments.
“The use of monitoring substrates for adjusting the protein transport capacity has also been shown in Vibrio alginolyticus, where the substrate VemP controls the switch between a sodium-coupled SecDF2 complex and a proton-coupled SecDF1 complex in low Na+ environments (Ishii et al., 2015; Miyazaki et al., 2020).”
From The Dynamic SecYEG Translocon (2021) · cites this paper as Ishii et al., 2015
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Self-citation
- Authors
- Ishii, E., Chiba, S., Hashimoto, N., Kojima, S., Homma, M., Ito, K., Akiyama, Y., & Mori, H.
- Affiliations
- Kyoto University (JP)
- Kyoto Sangyo University (JP)
- Nagoya University (JP)
- Corresponding author
- Mori, Hiroyuki
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2015-09-21
- Volume
- 112
- Issue
- 40
- Pages
- E5513-22
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC4603450
- OpenAlex
- W2150104949
- Cited by
- 65
- References
- 73
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 11 · 2023: 7 · 2022: 6 · 2021: 8 · 2020: 6 · 2019: 2 · 2018: 8 · 2017: 5 · 2016: 5
- FWCI
- 4.11
- Type
- journal article
- Language
- en
- Update notices
- correction (2015-12-14)
- Open access
- bronze
- doi.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science, and Technology (24117003, 15H01532, 26116008)
- Japan Society for the Promotion of Science (22370070, 25291006)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- SecDF, SecYEG translocon, protein export, Vibrio alginolyticus, salinity adaptation, proton motive force, vestibular evoked myogenic potentials, gene expression regulation, marine bacteria, paralogs
- MeSH terms
- Amino Acid Sequence, Bacterial Proteins, Base Sequence, Molecular Sequence Data, Mutation, Nucleic Acid Conformation, Ribosomes, RNA, Messenger, Seawater, Sodium, Protein Biosynthesis, Vibrio, Immunoblotting, Gene Expression Regulation, Bacterial, Sequence Homology, Amino Acid, Proton-Motive Force, Protein Transport, Salinity, Salt Tolerance
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