The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
Small fluorescence-activating and absorption-shifting tag for tunable protein imaging in vivo
Open accessAbstract
Significance: We developed a small protein tag enabling fluorescent labeling of proteins in living cells and in multicellular organisms through the specific binding and activation of a cell-permeant and nontoxic fluorogenic ligand. This tag, called Yellow Fluorescence-Activating and absorption-Shifting Tag (Y-FAST), was engineered by directed evolution from the Photoactive Yellow Protein. Y-FAST distinguishes itself from other labeling methods because the fluorogen binding is highly dynamic and fully reversible. Apart from providing new opportunities in superresolution imaging and biosensor design, this feature enables rapid switching on and off of the fluorescence of a fusion protein by addition or withdrawing of the fluorogenic ligand, opening exciting ways to perform sequential multiplexing imaging.
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Cites
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“However, protein copy numbers can vary by six orders of magnitude in mammalian cells (37), surpassing the dynamic range of most fluorescence detectors (typically three orders of magnitude).”
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Cited by
De novo design of a fluorescence-activating β-barrel
Dou, J., Vorobieva, A. A., Sheffler, W., Doyle, L. A., Park, H., Bick, M. J., Mao, B., Foight, G. W., Lee, M. Y., Gagnon, L. A., et al. (2018) · Nature · doi:10.1038/s41586-018-0509-0 · Cited by 351
Bottone, S., Joliot, O., Cakil, Z. V., El Hajji, L., Rakotoarison, L.-M., Boncompain, G., Perez, F., & Gautier, A. (2023) · Nature Methods · doi:10.1038/s41592-023-01988-8 · Cited by 48
Self-citationA fluorogenic complementation tool kit for interrogating lipid droplet–organelle interaction
Li, X., Gamuyao, R., Wu, M.-L., Cho, W. J., King, S. V., Petersen, R. A., Stabley, D. R., Lindow, C., Climer, L. K., Shirinifard, A., et al. (2024) · Journal of Cell Biology · doi:10.1083/jcb.202311126 · Cited by 31
(2025) · doi:10.1038/s41467-025-62241-8 · Cited by 5
Metadata
- Authors
- Plamont, M.-A., Billon-Denis, E., Maurin, S., Gauron, C., Pimenta, F. M., Specht, C. G., Shi, J., Quérard, J., Pan, B., Rossignol, J., Moncoq, K., Morellet, N., Volovitch, M., Lescop, E., Chen, Y., Triller, A., Vriz, S., Le Saux, T., Jullien, L., & Gautier, A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2015-12-28
- Volume
- 113
- Issue
- 3
- Pages
- 497-502
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2196795138
- Cited by
- 286
- References
- 44
- FWCI
- 7.36
- Type
- journal article
- Language
- en
- Open access
- green
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- Plamont, M.-A., Billon-Denis, E., Maurin, S., Gauron, C., Pimenta, F. M., Specht, C. G., Shi, J., Quérard, J., Pan, B., Rossignol, J., Moncoq, K., Morellet, N., Volovitch, M., Lescop, E., Chen, Y., Triller, A., Vriz, S., Le Saux, T., Jullien, L., & Gautier, A. (2016). Small fluorescence-activating and absorption-shifting tag for tunable protein imaging in vivo. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1513094113
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