Cited by 211Archaeology and ancient environmental studiesArchaeological and Geological StudiesAncient and Medieval Archaeology Studies

The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index; none of the 22 sources its claims cite is checked for support yet.

Revealing a 5,000-y-old beer recipe in China

Open access
Wang, Jiajing, Liu, Li, Ball, Terry, Yu, Linjie, Li, Yuanqing, Xing, Fulai·2016·Proceedings of the National Academy of Sciences

Abstract

The pottery vessels from the Mijiaya site reveal, to our knowledge, the first direct evidence of in situ beer making in China, based on the analyses of starch, phytolith, and chemical residues. Our data reveal a surprising beer recipe in which broomcorn millet ( Panicum miliaceum ), barley ( Hordeum vulgare ), Job’s tears ( Coix lacryma-jobi ), and tubers were fermented together. The results indicate that people in China established advanced beer-brewing technology by using specialized tools and creating favorable fermentation conditions around 5,000 y ago. Our findings imply that early beer making may have motivated the initial translocation of barley from the Western Eurasia into the Central Plain of China before the crop became a part of agricultural subsistence in the region 3,000 y later.

Overview

AI snapshot

Written from the paper’s full text.

TL;DR

Residue analyses of Mijiaya pottery provide the earliest direct evidence of in situ beer making in China, with a mixed recipe of broomcorn millet, barley, Job’s tears, and tubers around 5,000 y ago. The barley evidence predates macrobotanical remains in China by 1,000 y and suggests beer making may have motivated barley’s initial translocation before it became a subsistence crop.

Key findings

  • Starch, phytolith, and chemical residues from Mijiaya vessels indicate a fermented beverage made from broomcorn millet (*Panicum miliaceum*), barley (*Hordeum vulgare*), Job’s tears (*Coix lacryma-jobi*), and tubers including snake gourd root, yam, and lily.
  • A total of 541 starch grains were recovered; 488 (90.2%) were identifiable, and 166 (30.7%) showed damage patterns matching malting and mashing. Phytoliths included η-shaped broomcorn millet elements, Job’s tears crosses, and articulated dendritic Triticeae phytoliths most probably from barley.
  • Ion chromatography detected oxalate in Funnel 1 at 0.08% (80 mg/100 g) and Pot 5 at 0.05% (50 mg/100 g), but not in Pot 3; control samples showed little or no comparable residues.
  • Barley at Mijiaya is reported as the earliest occurrence of this crop in China, predating macrobotanical remains by 1,000 y; the authors suggest barley was initially introduced to the Central Plain as an alcohol ingredient rather than for subsistence.

Methods

  • Residue analyses—starch, phytolith, and ion chromatography—were conducted on two complete funnels and pottery sherds from five *jiandiping* amphorae and two wide-mouth pots from Mijiaya pits H82 and H78, dated to 3400–2900 BC (Banpo IV/late Yangshao), with four control samples.
  • Starch and phytolith identification used morphological comparison with a reference collection of over 1,000 Asian and European economically important plant specimens and morphometric methods: a discriminant model separating Job’s tears from millets with 82.4% success, and articulated dendritic phytolith wave-lobe measurements with at least 30 measurable lobes per residue sample compared against 20 Triticeae and other dendritic-producing species.
  • Oxalate was measured by ion chromatography on residues from Funnel 1, Pot 3, and Pot 5, with control sample 4 analyzed separately.

Limitations

  • The paper states that future research with more comprehensive phytolith data from other Neolithic contexts is needed to test the hypothesis, and notes this is the first study applying morphometric analysis to dendritic phytoliths from China.
  • Oxalate was not detected in Pot 3, and only three residue samples were tested by ion chromatography for oxalate; the authors also acknowledge that oxalates occur naturally in some plants, though they argue the vessel types are not suitable for storing fresh plant material.
  • Evident from the text: identifications rely partly on morphometric models and reference collections with stated success rates, such as 82.4% for separating Job’s tears from millets, and the “beer-making toolkit” interpretation rests on artifact assemblages from two pits rather than direct ancient DNA or macrobotanical barley remains.

Model-written summary. Check it against the paper before relying on it.

Integrity signals

Registry

Crossref · 10.1073/pnas.1601465113

Cites

29 of 29 references verified0 citation errors · 0 not checkable

Statistics

Nothing to recompute.

Verification

Claims the paper makes

0 of 22 cited sources checked · 0 supported · 0 flagged

Show all 36 claims

  • It may state two separate claims as one. 19
  • The quote only partly states this claim. 1
  • Probably not a substantial claim. 6
23 of 23
  • The paper relies on this without support.

    Direct evidence for alcohol production from Yangshao sites is lacking.

    “However, direct evidence for alcohol production from Yangshao sites is lacking.”
  • The paper relies on this without support.

    The Mijiaya vessel types are not suitable for storing any fresh plant.

    “Although oxalates occur naturally in plants like spinach (Spinacia oleracea), rhubarb (Rheum rhabarbarum), and some Dioscorea and Lilium species, the vessel types in this study are not suitable for storing any fresh plant.”
  • The paper relies on this without support.

    Barley was first domesticated in Western Eurasia and later introduced into China, presumably through the Central Asian steppe.

    “Barley was first domesticated in Western Eurasia and later introduced into China, presumably through the Central Asian steppe.”
3 of 3

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

  • Triticeae percentage is inconsistent with the table totals

    Minor

    The table reports 152 Triticeae grains out of 541 total grains. That is 28.1%, not 28.61%.

    Reported 28.61 · recomputed 28.096118

  • Pot 3 starch counts do not sum to the reported total

    Minor

    The counts in the Pot 3 row sum to 37, not 38.

    Reported 38 · recomputed 37

  • Pot 5 starch counts do not sum to the reported total

    Minor

    The counts in the Pot 5 row sum to 64, not 66.

    Reported 66 · recomputed 64

  • Pot 7 starch counts do not sum to the reported total

    Minor

    The counts in the Pot 7 row sum to 22, not 24.

    Reported 24 · recomputed 22

4 of 4

Citations

Cites

Flagged first, then by citations

Show all 28 references

Research works printed but not linked: 13

  1. S Wen, T Yuan Yinxu Buci Yanjiu: Kexue Jishu Pian (A Study of Oracle Bones from Yinxu: Science and Technology) (Sichuansheng Shehui Kexueyuan, 1st Ed, Chengdu, China, 1983).
  2. D Zhang, Yinshang jiuwenhua chulun (A preliminary study of Shang alcohol culture). Zhongyuan Wenwu 3, 19–24 (1994).
  3. M Chen Yinxu Buci Zongshu (A Comprehensive Study of the Oracle Bone Inscriptions from Yinxu) (Kexue, Beijing, 1956).
  4. HT Huang Science and Civilisation in China. Vol 6: Biology and Biological Technology. Part V: Fermentations and Food Science (Cambridge Univ Press, Cambridge, UK, 2000).
  5. Y Li, Dui woguo niangjiu qiyuan de tantao (The origin of alcoholic beverages in China). Kaogu 1, 41–44 (1962).
  6. Q Bao, J Zhou Zhongguo Chuantong Gongyi Quanji: Niangzao (Chinese Traditional Technology: Brewing) (Daxiang, 1st Ed, Zhengzhou, China, 2007).
  7. ; Shaanxi Provincial Institute of Archaeology Xi’an Mijiaya–Xinshiqi Shidai Yizhi 2004-2006 Kaogu Fajue Baogao (2004–2006 Excavation Report of the Neolithic Site Mijiaya in Xi’an) (Kexue, Beijing, 2012).
  8. ; Institute of Archaeology, Chinese Academy of Social Sciences Zhongguo Kaoguxue Zhong Tanshisi Niandai Shujuji (Radiocarbon Dates in Chinese Archaeology 1965–1991) (Cultural Relics Publishing House, Beijing, 1991).
  9. Y Yang Shaanxi Yangguanzhai Yizhi Yangshao Wenhua Zhongwanqi Qihou Huanjing Jilu Ji Yizhi Gurenlei Dui Quyu Huanjin Yicun (The Climatic Record and Human-environmental Relations from Middle to Late Yangshao Period: A Case Study at Yangguanzhai Shaanxi). Master’s thesis (Xibei University Xi’an China). (2013).
  10. BL Dronzek, P Hwang, W Bushuk, Scanning electron microscopy of starch from sprouted wheat. Cereal Chem 49, 232–239 (1972).
  11. X Liang, Shilun Shaanxi Miaodegou II culture (A Discussion on Shaanxi Miaodigou II Culture). Kaogu Xuebao 04, 397–412 (1987).
  12. X Liang, Shilun keshengzhuang Erqi wenhua (A discussion on the Keshengzhuang Culture). Kaogu Xuebao 04, 397–424 (1994).
  13. ; GPICRA (Gansu Provincial Institute of Cultural Relics and Archaeology) Gansu Qin’an Dadiwan Xinshiqi Shidai Yizhi Fajue Baogao (Qin’an Dadiwan Excavation Report) (Cultural Relics Publishing House, Beijing, 2006).

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Metadata

Authors
Wang, J., Liu, L., Ball, T., Yu, L., Li, Y., & Xing, F.
Affiliations
  • Stanford University (US)
  • Stanford Archaeology Center, Stanford University, Stanford, CA 94305;
  • Brigham Young University (US)
  • Zhejiang Research Institute of Chemical Industry (CN)
  • Shaanxi Provincial Institute of Archaeology, 710054 Xi’an, China
Corresponding author
Wang, Jiajing
Venue
Proceedings of the National Academy of Sciences
Published
2016-05-23
Volume
113
Issue
23
Pages
6444-6448
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2398626377
Cited by
206
References
28
Citations by year
2026: 12 · 2025: 13 · 2024: 35 · 2023: 20 · 2022: 30 · 2021: 24 · 2020: 32 · 2019: 18 · 2018: 13 · 2017: 9
FWCI
11.09
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • ScholarsArchive (Brigham Young University) (submittedVersion, cc-by-nc-nd)
Topics
  • Social Sciences › Archeology › Archaeology and ancient environmental studies
  • Social Sciences › Arts and Humanities › Archeology (arts and humanities) › Archaeological and Geological Studies
  • Social Sciences › Arts and Humanities › Archeology (arts and humanities) › Ancient and Medieval Archaeology Studies
Keywords
starch grain analysis, phytoliths, chemical residues, Panicum miliaceum, barley, Coix lacryma-jobi, tubers, fermentation
MeSH terms
Beer, Edible Grain, China, Magnoliopsida, Plant Tubers, History, Ancient

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