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Neural tube morphogenesis in synthetic 3D microenvironments
Open accessAbstract
Significance: In vitro organoids have become widely used model systems in basic research and for therapeutic applications due to their ability to recapitulate key elements of in vivo form and function. However, their full potential remains unfulfilled as a result of the poorly defined matrices in which they are grown. Here, we use modular synthetic 3D matrices to show that early neural morphogenesis can be precisely controlled by the extracellular microenvironment. Our approach is broadly applicable to gain a broader understanding of the multifactorial 3D cell–matrix interactions that coordinate multicellular growth and differentiation, and opens up avenues to discover and dissect the unique microenvironments that control morphogenesis in various organoid systems.
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Designing materials for biology and medicine
Langer, R., & Tirrell, D. A. (2004) · Nature · doi:10.1038/nature02388 · Cited by 3,198
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Griffith, L. G., & Swartz, M. A. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1858 · Cited by 2,347
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Takebe, T., Sekine, K., Enomura, M., Koike, H., Kimura, M., Ogaeri, T., Zhang, R.-R., Ueno, Y., Zheng, Y.-W., Koike, N., et al. (2013) · Nature · doi:10.1038/nature12271 · Cited by 2,170
Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture
Ying, Q.-L., Stavridis, M., Griffiths, D., Li, M., & Smith, A. (2003) · Nature Biotechnology · doi:10.1038/nbt780 · Cited by 1,504
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Lütolf, M. P., Gilbert, P. M., & Blau, H. M. (2009) · Nature · doi:10.1038/nature08602 · Cited by 1,409
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Lukinavičius, G., Reymond, L., D’Este, E., Masharina, A., Göttfert, F., Ta, H., Güther, A., Fournier, M., Rizzo, S., Waldmann, H., et al. (2014) · Nature Methods · doi:10.1038/nmeth.2972 · Cited by 822
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Eyckmans, J., Boudou, T., Yu, X., & Chen, C. S. (2011) · Developmental Cell · doi:10.1016/j.devcel.2011.06.015 · Cited by 527
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Metadata
- Authors
- Ranga, A., Girgin, M., Meinhardt, A., Eberle, D., Caiazzo, M., Tanaka, E. M., & Lutolf, M. P.
- Affiliations
- École Polytechnique Fédérale de Lausanne (CH)
- Center for Regenerative Therapies Dresden (DE)
- Laboratory of Stem Cell Bioengineering, Institute of Bioengineering, School of Life Sciences and School of Engineering, Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland;
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2016-10-14
- Volume
- 113
- Issue
- 44
- Pages
- E6831-E6839
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC5098636
- OpenAlex
- W2531835077
- Cited by
- 221
- References
- 32
- Citations by year
- 2026: 7 · 2025: 13 · 2024: 21 · 2023: 29 · 2022: 31 · 2021: 29 · 2020: 30 · 2019: 21 · 2018: 24 · 2017: 15 · 2016: 1
- FWCI
- 13.84
- Type
- journal article
- Language
- en
- Update notices
- correction (2017-04-10)
- Open access
- bronze
- doi.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Utrecht University Repository (Utrecht University) (submittedVersion, other-oa)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
- Lirias (submittedVersion)
- Funders
- EU FP7 (PluriMes)
- SystemX.ch (StoNets)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- neural tube morphogenesis, synthetic extracellular matrix, 3D organoids, neuroepithelial cyst, early neurogenesis, dorsoventral patterning, symmetry breaking, extracellular matrix mechanics, organogenesis
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