The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
A chemical approach for identifying O -GlcNAc-modified proteins in cells
Open accessAbstract
The glycosylation of serine and threonine residues with a single GlcNAc moiety is a dynamic posttranslational modification of many nuclear and cytoplasmic proteins. We describe a chemical strategy directed toward identifying O -GlcNAc-modified proteins from living cells or proteins modified in vitro . We demonstrate, in vitro , that each enzyme in the hexosamine salvage pathway, and the enzymes that affect this dynamic modification (UDP-GlcNAc:polypeptidtyltransferase and O -GlcNAcase), tolerate analogues of their natural substrates in which the N -acyl side chain has been modified to bear a bio-orthogonal azide moiety. Accordingly, treatment of cells with N -azidoacetylglucosamine results in the metabolic incorporation of the azido sugar into nuclear and cytoplasmic proteins. These O -azidoacetylglucosamine-modified proteins can be covalently derivatized with various biochemical probes at the site of protein glycosylation by using the Staudinger ligation. The approach was validated by metabolic labeling of nuclear pore protein p62, which is known to be posttranslationally modified with O -GlcNAc. This strategy will prove useful for both the identification of O -GlcNAc-modified proteins and the elucidation of the specific residues that bear this saccharide.
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Cites
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Dynamic O-Glycosylation of Nuclear and Cytosolic Proteins
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Cited by
Bioorthogonal Chemistry: Fishing for Selectivity in a Sea of Functionality
Sletten, E. M., & Bertozzi, C. R. (2009) · Angewandte Chemie International Edition · doi:10.1002/anie.200900942 · Cited by 3,165
Self-citationPrescher, J. A., & Bertozzi, C. R. (2005) · Nature Chemical Biology · doi:10.1038/nchembio0605-13 · Cited by 1,456
Self-citationCycling of O-linked β-N-acetylglucosamine on nucleocytoplasmic proteins
Hart, G. W., Housley, M. P., & Slawson, C. (2007) · Nature · doi:10.1038/nature05815 · Cited by 1,418
A metabolic labeling approach toward proteomic analysis of mucin-type O-linked glycosylation
Hang, H. C., Yu, C., Kato, D. L., & Bertozzi, C. R. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2335201100 · Cited by 580
Self-citationBioorthogonal Reactions for Labeling Proteins
Lang, K., & Chin, J. W. (2014) · ACS Chemical Biology · doi:10.1021/cb4009292 · Cited by 555
Metadata
- Authors
- Vocadlo, D. J., Hang, H. C., Kim, E.-J., Hanover, J. A., & Bertozzi, C. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2003-07-21
- Volume
- 100
- Issue
- 16
- Pages
- 9116-9121
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2089693526
- Cited by
- 538
- References
- 38
- FWCI
- 6.00
- Type
- journal article
- Language
- en
- Open access
- green
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