The DOI matches its Crossref record.
Sex determination of human remains from peptides in tooth enamel
Abstract
Significance: The ability to assign biological sex to human skeletal remains is a fundamental requirement in archaeology, paleoanthropology, and medico-legal sciences. While DNA sequencing can be used, it is expensive, time-consuming, and often fails due to the poor quality of the remaining DNA. An easier, more reliable, and consistently applicable method is needed. We present a method for sex determination of human remains using peptides retrieved from tooth enamel. Amelogenin is an enamel-forming protein encoded for by both chromosomes X and Y, with slight differences in their amino acid sequences. Peptides with these differences were identified by nanoflow liquid chromatography mass spectrometry and found to correctly assign sex to archaeological human remains of various chronological ages, from hundreds to thousands of years old.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Enamel has been explored as a well-preserved source of ancient proteins that can be analyzed to enable phylogenetic analysis of extinct animals and humans, as well as identification of sex based on sequence differences in amelogenin.
“Recently, enamel has been explored as a well-preserved source of ancient proteins that can be analyzed to enable the phylogenetic analysis of extinct animals and humans (71, 72), as well as the identification of sex based on sequence differences in the enamel protein amelogenin (73–75).”
From Ancient protein analysis in archaeology (2021) · cites this paper as [74]
More sensitive MS techniques have enabled increased protein sequence coverage.
“More recently, more sensitive MS techniques have enabled increased protein sequence coverage”
From Ancient protein analysis in archaeology (2021) · cites this paper as [74]
The Materials and Methods section describes the nanoLC-MS/MS equipment and analysis, and the Results and Discussion section reports the enamel peptides identified. Neither passage addresses whether more sensitive MS techniques increased protein sequence coverage; searches for sensitivity and sequence coverage found no such comparison in our copy. (LLM, conf=0.70)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
No references could be resolved yet.
Research works printed but not linked: 17
- R Gowland, TJU Thompson Human Identity and Identification (Cambridge Univ Press, Cambridge, UK, 2013). · DOI
- M Cox, S Mays Human Osteology in Archaeology and Forensic Science (Greenwich Medical Media, London), pp. 117–120 (2000).
- JE Buikstra, DH Ubelaker Standards for Data Collection from Human Skeletal Remains: Proceedings of a Seminar at the Field Museum of Natural History (Arkansas Archeological Survey, Fayetteville, AR, 1994).
- H Schutkowski, Sex determination of infant and juvenile skeletons, I: Morphognostic features. Am J Phys Anthropol 90, 199–205 (1993). · DOI
- D Vlak, M Roksandic, MA Schillaci, Greater sciatic notch as a sex indicator in juveniles. Am J Phys Anthropol 137, 309–315 (2008). · DOI
- M Lewis The Bioarchaeology of Children: Perspectives from Biological and Forensic Anthropology (Cambridge Univ Press, Cambridge, UK, 2007).
- N Abu-Mandil Hassan, KA Brown, J Eyers, TA Brown, S Mays, Ancient DNA study of the remains of putative infanticide victims from the Yewden Roman villa site at Hambleden, England. J Archaeol Sci 43, 192–197 (2014). · DOI
- S Mays, M Faerman, Sex identification in some putative infanticide victims from Roman Britain using ancient DNA. J Archaeol Sci 28, 555–559 (2001). · DOI
- NA Stewart, , The identification of peptides by nanoLC-MS/MS from human surface tooth enamel following a simple acid etch extraction. RSC Adv 6, 61673–61679 (2016). · DOI
- GA Castiblanco, , Identification of proteins from human permanent erupted enamel. Eur J Oral Sci 123, 390–395 (2015). · DOI
- IM Porto, HJ Laure, FB de Sousa, JC Rosa, RF Gerlach, New techniques for the recovery of small amounts of mature enamel proteins. J Archaeol Sci 38, 3596–3604 (2011). · DOI
- IM Porto, , Recovery and identification of mature enamel proteins in ancient teeth. Eur J Oral Sci 119, 83–87 (2011). · DOI · Paper page
- A Caffell, M Holst Osteological analysis, the Church of Saint Michael and Saint Lawrence, Fewston, UK (York Osteoarchaeology Ltd., York, UK, 2010).
- WM Bass Human Osteology: A Laboratory and Field Manual (Missouri Archaeological Society, 3rd Ed, Columbia, MO, 1987).
- AT Chamberlain, AV Witkin, A Neolithic cairn at Whitwell, Derbyshire. Derbyshire Archaeol J 131, 1–131 (2011).
- T Loeffelmann Of doomed and transient men: The Anglo-Saxon skeletons of Seaham in their context. MSc dissertation (Durham University Durham UK). (2014).
- JA Vizcaíno, , 2016 update of the PRIDE database and its related tools. Nucleic Acids Res 44, 11033 (2016). · DOI
Cited by
Sorted by citations
Ancient protein analysis in archaeology
Hendy, J. (2021) · Science Advances · doi:10.1126/sciadv.abb9314 · Cited by 161
Ancient Biomolecules and Evolutionary Inference
Cappellini, E., Prohaska, A., Racimo, F., Welker, F., Pedersen, M. W., Allentoft, M. E., de Barros Damgaard, P., Gutenbrunner, P., Dunne, J., Hammann, S., et al. (2018) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-062917-012002 · Cited by 118
Metadata
- Authors
- Stewart, N. A., Gerlach, R. F., Gowland, R. L., Gron, K. J., & Montgomery, J.
- Affiliations
- School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton BN2 4AT, United Kingdom;
- Department of Morphology, Physiology, and Basic Pathology, School of Dentistry of Ribeirão Preto, University of São Paulo, FORP/USP, 807 São Paulo, Brazil;
- Department of Archaeology, Durham University, Durham DH1 3LE, United Kingdom
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2017-12-11
- Volume
- 114
- Issue
- 52
- Pages
- 13649-13654
- ISSN
- 0027-8424
- 1091-6490
- PMC
- PMC5748210
- Cited by
- 208
- References
- 0
- Language
- en
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
