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Cited by 384

The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.

An acquired and a native penicillin-binding protein cooperate in building the cell wall of drug-resistant staphylococci

Open access
Pinho, Mariana G., de Lencastre, Hermínia, Tomasz, Alexander·2001·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.191260798
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Abstract

The blanket resistance of methicillin-resistant Staphylococcus aureus to all β-lactam antibiotics—which had such a devastating impact on chemotherapy of staphylococcal infections—is related to the properties of the key component of this resistance mechanism: the “acquired” penicillin-binding protein (PBP)-2A, which has unusual low affinity for all β-lactam antibiotics. Until now, the accepted model of resistance implied that in the presence of β-lactam antibiotics in the surrounding medium, PBP2A must take over the biosynthesis of staphylococcal cell wall from the four native staphylococcal PBPs because the latter become rapidly acylated and inactivated at even low concentrations of the antibiotic. However, recent observations indicate that this model requires revision. Inactivation of the transglycosylase domain, but not the transpeptidase domain, of PBP2 of S. aureus prevents expression of β-lactam resistance, despite the presence of the low-affinity PBP2A. The observations suggest that cell-wall synthesis in the presence of β-lactam antibiotics requires the cooperative functioning of the transglycosylase domain of the native staphylococcal PBP2 and the transpeptidase domain of the PBP2A, a protein imported by S. aureus from an extra species source.

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Metadata

Authors
Pinho, M. G., de Lencastre, H., & Tomasz, A.
Venue
Proceedings of the National Academy of Sciences
Publisher
Proceedings of the National Academy of Sciences
Published
2001-08-21
Volume
98
Issue
19
Pages
10886-10891
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.191260798
OpenAlex
W2083435927
Cited by
384
References
22
FWCI
9.28
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: An acquired and a native penicillin-binding protein cooperate in building the cell wall of drug-resistant staphylococci. https://app.cochat.ai/papers/10.1073/pnas.191260798
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Pinho, M. G., de Lencastre, H., & Tomasz, A. (2001). An acquired and a native penicillin-binding protein cooperate in building the cell wall of drug-resistant staphylococci. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.191260798

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