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Embryonic stem cells can form germ cells in vitro
Open accessAbstract
Knock-in embryonic stem (ES) cells, in which GFP or lacZ was expressed from the endogenous mouse vasa homolog ( Mvh ), which is specifically expressed in differentiating germ cells, were used to visualize germ cell production during in vitro differentiation. The appearance of MVH-positive germ cells depended on embryoid body formation and was greatly enhanced by the inductive effects of bone morphogenic protein 4-producing cells. The ES-derived MVH-positive cells could participate in spermatogenesis when transplanted into reconstituted testicular tubules, demonstrating that ES cells can produce functional germ cells in vitro . In vitro germ cell differentiation provides a paradigm for studying the molecular basis of germ line establishment, as well as for developing new approaches to reproductive engineering.
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Cited by
Xu, Q., Bernardo, A., Walker, D., Kanegawa, T., Mahley, R. W., & Huang, Y. (2006) · The Journal of Neuroscience · doi:10.1523/jneurosci.5476-05.2006 · Cited by 524
From teratocarcinomas to embryonic stem cells and beyond: a history of embryonic stem cell research
Solter, D. (2006) · Nature Reviews Genetics · doi:10.1038/nrg1827 · Cited by 326
In vitro production of fertile sperm from murine spermatogonial stem cell lines
Sato, T., Katagiri, K., Yokonishi, T., Kubota, Y., Inoue, K., Ogonuki, N., Matoba, S., Ogura, A., & Ogawa, T. (2011) · Nature Communications · doi:10.1038/ncomms1478 · Cited by 228
Resurrecting biodiversity: Advanced assisted reproductive technologies and biobanking
Bolton, R. L., Mooney, A., Pettit, M. T., Bolton, A. E., Morgan, L., Drake, G. J., Appeltant, R., Walker, S. L., Gillis, J. D., & Hvilsom, C. (2022) · Reproduction and Fertility · doi:10.1530/raf-22-0005 · Cited by 108
Metadata
- Authors
- Toyooka, Y., Tsunekawa, N., Akasu, R., & Noce, T.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2003-09-22
- Volume
- 100
- Issue
- 20
- Pages
- 11457-11462
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2143023822
- Cited by
- 700
- References
- 0
- FWCI
- 15.01
- Type
- journal article
- Language
- en
- Open access
- green
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- Toyooka, Y., Tsunekawa, N., Akasu, R., & Noce, T. (2003). Embryonic stem cells can form germ cells in vitro. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1932826100
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
