The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Widespread cytoplasmic mRNA transport in yeast: Identification of 22 bud-localized transcripts using DNA microarray analysis
Open accessAbstract
Cytoplasmic mRNA localization provides a means of generating cell asymmetry and segregating protein activity. Previous studies have identified two mRNAs that localize to the bud tips of the yeast Saccharomyces cerevisiae . To identify additional localized mRNAs, we immunoprecipitated the RNA transport components She2p, She3p, and Myo4p and performed DNA microarray analysis of their associated RNAs. A secondary screen, using a GFP-tagged RNA reporter assay, identified 22 mRNAs that are localized to bud tips. These messages encode a wide variety of proteins, including several involved in stress responses and cell wall maintenance. Many of these proteins are asymmetrically localized to buds. However, asymmetric localization also occurs in the absence of RNA transport, suggesting the existence of redundant protein localization mechanisms. In contrast to findings in metazoans, the untranslated regions are dispensable for mRNA localization in yeast. This study reveals an unanticipated widespread use of RNA transport in budding yeast.
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Cites
Visualization of Single RNA Transcripts in Situ
Femino, A. M., Fay, F. S., Fogarty, K., & Singer, R. H. (1998) · Science · doi:10.1126/science.280.5363.585 · Cited by 1,504
Cited by
Hogan, D. J., Riordan, D. P., Gerber, A. P., Herschlag, D., & Brown, P. O. (2008) · PLoS Biology · doi:10.1371/journal.pbio.0060255 · Cited by 609
Self-citationArchitecture and Biosynthesis of the Saccharomyces cerevisiae Cell Wall
Orlean, P. (2012) · Genetics · doi:10.1534/genetics.112.144485 · Cited by 478
Organization and Function of Membrane Contact Sites
Helle, S. C. J., Kanfer, G., Kolar, K., Lang, A., Michel, A. H., & Kornmann, B. (2013) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2013.01.028 · Cited by 453
Intracellular mRNA transport and localized translation
Das, S., Vera, M., Gandin, V., Singer, R. H., & Tutucci, E. (2021) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-021-00356-8 · Cited by 382
Post-transcriptional control of gene expression: a genome-wide perspective
Mata, J., Marguerat, S., & Bähler, J. (2005) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2005.07.005 · Cited by 288
Metadata
- Authors
- Shepard, K. A., Gerber, A. P., Jambhekar, A., Takizawa, P. A., Brown, P. O., Herschlag, D., DeRisi, J. L., & Vale, R. D.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2003-09-17
- Volume
- 100
- Issue
- 20
- Pages
- 11429-11434
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2075639867
- Cited by
- 284
- References
- 1
- FWCI
- 5.58
- Type
- journal article
- Language
- en
- Open access
- green
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- Shepard, K. A., Gerber, A. P., Jambhekar, A., Takizawa, P. A., Brown, P. O., Herschlag, D., DeRisi, J. L., & Vale, R. D. (2003). Widespread cytoplasmic mRNA transport in yeast: Identification of 22 bud-localized transcripts using DNA microarray analysis. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.2033246100
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