The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
The binding specificity and affinity determinants of family 1 and family 3 cellulose binding modules
Open accessAbstract
Cellulose binding modules (CBMs) potentiate the action of cellulolytic enzymes on insoluble substrates. Numerous studies have established that three aromatic residues on a CBM surface are needed for binding onto cellulose crystals and that tryptophans contribute to higher binding affinity than tyrosines. However, studies addressing the nature of CBM–cellulose interactions have so far failed to establish the binding site on cellulose crystals targeted by CBMs. In this study, the binding sites of CBMs on Valonia cellulose crystals have been visualized by transmission electron microscopy. Fusion of the CBMs with a modified staphylococcal protein A (ZZ-domain) allowed direct immuno-gold labeling at close proximity of the actual CBM binding site. The transmission electron microscopy images provide unequivocal evidence that the fungal family 1 CBMs as well as the family 3 CBM from Clostridium thermocellum CipA have defined binding sites on two opposite corners of Valonia cellulose crystals. In most samples these corners are worn to display significant area of the hydrophobic (110) plane, which thus constitutes the binding site for these CBMs.
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Citations
Cites
Sugiyama, J., Vuong, R., & Chanzy, H. (1991) · Macromolecules · doi:10.1021/ma00014a033 · Cited by 791
Self-citation
Cited by
Zhang, Y. P., & Lynd, L. R. (2004) · Biotechnology and Bioengineering · doi:10.1002/bit.20282 · Cited by 1,893
Deconstruction of Lignocellulosic Biomass to Fuels and Chemicals
Chundawat, S. P. S., Beckham, G. T., Himmel, M. E., & Dale, B. E. (2011) · Annual Review of Chemical and Biomolecular Engineering · doi:10.1146/annurev-chembioeng-061010-114205 · Cited by 1,017
Payne, C. M., Knott, B. C., Mayes, H. B., Hansson, H., Himmel, M. E., Sandgren, M., Ståhlberg, J., & Beckham, G. T. (2015) · Chemical Reviews · doi:10.1021/cr500351c · Cited by 855
Computer simulation studies of microcrystalline cellulose Iβ
Matthews, J. F., Skopec, C. E., Mason, P. E., Zuccato, P., Torget, R. W., Sugiyama, J., Himmel, M. E., & Brady, J. W. (2006) · Carbohydrate Research · doi:10.1016/j.carres.2005.09.028 · Cited by 398
Self-citationCrystal Structure and Binding Properties of the Serratia marcescens Chitin-binding Protein CBP21
Vaaje-Kolstad, G., Houston, D. R., Riemen, A. H. K., Eijsink, V. G. H., & van Aalten, D. M. F. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m407175200 · Cited by 314
Metadata
- Authors
- Lehtiö, J., Sugiyama, J., Gustavsson, M., Fransson, L., Linder, M., & Teeri, T. T.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2003-01-09
- Volume
- 100
- Issue
- 2
- Pages
- 484-489
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2095205431
- Cited by
- 358
- References
- 1
- FWCI
- 8.45
- Type
- journal article
- Language
- en
- Open access
- green
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- Lehtiö, J., Sugiyama, J., Gustavsson, M., Fransson, L., Linder, M., & Teeri, T. T. (2003). The binding specificity and affinity determinants of family 1 and family 3 cellulose binding modules. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.212651999
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