The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Distribution of NF-κB-binding sites across human chromosome 22
Open accessAbstract
We have mapped the chromosomal binding site distribution of a transcription factor in human cells. The NF-κB family of transcription factors plays an essential role in regulating the induction of genes involved in several physiological processes, including apoptosis, immunity, and inflammation. The binding sites of the NF-κB family member p65 were determined by using chromatin immunoprecipitation and a genomic microarray of human chromosome 22 DNA. Sites of binding were observed along the entire chromosome in both coding and noncoding regions, with an enrichment at the 5′ end of genes. Strikingly, a significant proportion of binding was seen in intronic regions, demonstrating that transcription factor binding is not restricted to promoter regions. NF-κB binding was also found at genes whose expression was regulated by tumor necrosis factor α, a known inducer of NF-κB-dependent gene expression, as well as adjacent to genes whose expression is not affected by tumor necrosis factor α. Many of these latter genes are either known to be activated by NF-κB under other conditions or are consistent with NF-κB's role in the immune and apoptotic responses. Our results suggest that binding is not restricted to promoter regions and that NF-κB binding occurs at a significant number of genes whose expression is not altered, thereby suggesting that binding alone is not sufficient for gene activation.
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Cites
Activators and target genes of Rel/NF-κB transcription factors
Pahl, H. L. (1999) · Oncogene · doi:10.1038/sj.onc.1203239 · Cited by 3,727
Transcriptional Regulatory Networks in Saccharomyces cerevisiae
Lee, T. I., Rinaldi, N. J., Robert, F., Odom, D. T., Bar-Joseph, Z., Gerber, G. K., Hannett, N. M., Harbison, C. T., Thompson, C. M., Simon, I., et al. (2002) · Science · doi:10.1126/science.1075090 · Cited by 2,956
Looping and Interaction between Hypersensitive Sites in the Active β-globin Locus
Tolhuis, B., Palstra, R.-J., Splinter, E., Grosveld, F., & de Laat, W. (2002) · Molecular Cell · doi:10.1016/s1097-2765(02)00781-5 · Cited by 1,344
E2F integrates cell cycle progression with DNA repair, replication, and G 2 /M checkpoints
Ren, B., Cam, H., Takahashi, Y., Volkert, T., Terragni, J., Young, R. A., & Dynlacht, B. D. (2002) · Genes & Development · doi:10.1101/gad.949802 · Cited by 1,125
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Transcriptional Regulatory Elements in the Human Genome
Maston, G. A., Evans, S. K., & Green, M. R. (2006) · Annual Review of Genomics and Human Genetics · doi:10.1146/annurev.genom.7.080505.115623 · Cited by 937
Circuitry of nuclear factor κB signaling
Hoffmann, A., & Baltimore, D. (2006) · Immunological Reviews · doi:10.1111/j.0105-2896.2006.00375.x · Cited by 907
Insights from genomic profiling of transcription factors
Farnham, P. J. (2009) · Nature Reviews Genetics · doi:10.1038/nrg2636 · Cited by 519
Xia, X., Lemieux, M. E., Li, W., Carroll, J. S., Brown, M., Liu, X. S., & Kung, A. L. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0810067106 · Cited by 410
Hollenhorst, P. C., Shah, A. A., Hopkins, C., & Graves, B. J. (2007) · Genes & Development · doi:10.1101/gad.1561707 · Cited by 290
Crosstalk via the NF-κB signaling system
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Neuroadaptations in Human Chronic Alcoholics: Dysregulation of the NF-κB System
Ökvist, A., Johansson, S., Kuzmin, A., Bazov, I., Merino-Martinez, R., Ponomarev, I., Mayfield, R. D., Harris, R. A., Sheedy, D., Garrick, T., et al. (2007) · PLoS ONE · doi:10.1371/journal.pone.0000930 · Cited by 84
The Activation of IL-1-Induced Enhancers Depends on TAK1 Kinase Activity and NF-κB p65
Jurida, L., Soelch, J., Bartkuhn, M., Handschick, K., Müller, H., Newel, D., Weber, A., Dittrich-Breiholz, O., Schneider, H., Bhuju, S., et al. (2015) · Cell Reports · doi:10.1016/j.celrep.2015.01.001 · Cited by 51
Metadata
- Authors
- Martone, R., Euskirchen, G., Bertone, P., Hartman, S., Royce, T. E., Luscombe, N. M., Rinn, J. L., Nelson, F. K., Miller, P., Gerstein, M., Weissman, S., & Snyder, M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2003-10-03
- Volume
- 100
- Issue
- 21
- Pages
- 12247-12252
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2154074258
- Cited by
- 305
- References
- 4
- FWCI
- 12.14
- Type
- journal article
- Language
- en
- Open access
- green
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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