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An optomechanical transducer in the blue light receptor phototropin from Avena sativa
Open accessAbstract
The PHOT1 ( NPH1 ) gene from Avena sativa specifies the blue light receptor for phototropism, phototropin, which comprises two FMN-binding LOV domains and a serine/threonine protein kinase domain. Light exposure is conducive to autophosphorylation of the protein kinase domain. We have reconstituted a recombinant LOV2 domain of A. sativa phototropin with various 13 C/ 15 N-labeled isotopomers of the cofactor, FMN. The reconstituted protein samples were analyzed by NMR spectroscopy under dark and light conditions. Blue light irradiation is shown to result in the addition of a thiol group (cysteine 450) to the 4a position of the FMN chromophore. The adduct reverts spontaneously in the dark by elimination. The light-driven flavin adduct formation results in conformational modification, which was diagnosed by 1 H and 31 P NMR spectroscopy. This conformational change is proposed to initiate the transmission of the light signal via conformational modulation of the protein kinase domain conducive to autophosphorylation of NPH1 .
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Citations
Cited by
Phototropins 1 and 2: versatile plant blue-light receptors
Briggs, W. R., & Christie, J. M. (2002) · Trends in Plant Science · doi:10.1016/s1360-1385(02)02245-8 · Cited by 785
Structural Basis of a Phototropin Light Switch
Harper, S. M., Neil, L. C., & Gardner, K. H. (2003) · Science · doi:10.1126/science.1086810 · Cited by 767
Structure and Signaling Mechanism of Per-ARNT-Sim Domains
Möglich, A., Ayers, R. A., & Moffat, K. (2009) · Structure · doi:10.1016/j.str.2009.08.011 · Cited by 531
Structure and function of plant photoreceptors
Möglich, A., Yang, X., Ayers, R. A., & Moffat, K. (2010) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-042809-112259 · Cited by 499
Cellular and Subcellular Localization of Phototropin 1
Sakamoto, K., & Briggs, W. R. (2002) · The Plant Cell · doi:10.1105/tpc.003293 · Cited by 405
Metadata
- Authors
- Salomon, M., Eisenreich, W., Dürr, H., Schleicher, E., Knieb, E., Massey, V., Rüdiger, W., Müller, F., Bacher, A., & Richter, G.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2001-10-16
- Volume
- 98
- Issue
- 22
- Pages
- 12357-12361
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2168836416
- Cited by
- 212
- References
- 0
- FWCI
- 23.40
- Type
- journal article
- Language
- en
- Open access
- green
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- Salomon, M., Eisenreich, W., Dürr, H., Schleicher, E., Knieb, E., Massey, V., Rüdiger, W., Müller, F., Bacher, A., & Richter, G. (2001). An optomechanical transducer in the blue light receptor phototropin from Avena sativa. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.221455298
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