The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Protein folding and unfolding in microseconds to nanoseconds by experiment and simulation
Open accessAbstract
The Engrailed Homeodomain protein has the highest refolding and unfolding rate constants directly observed to date. Temperature jump relaxation measurements gave a refolding rate constant of 37,500 s −1 in water at 25°C, rising to 51,000 s −1 around 42°C. The unfolding rate constant was 1,100 s −1 in water at 25°C and 205,000 s −1 at 63°C. The unfolding half-life is extrapolated to be ≈7.5 ns at 100°C, which allows real-time molecular dynamics unfolding simulations to be tested on this system at a realistic temperature. Preliminary simulations did indeed conform to unfolding on this time scale. Further, similar transition states were observed in simulations at 100°C and 225°C, suggesting that high-temperature simulations provide results applicable to lower temperatures.
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Kabsch, W., & Sander, C. (1983) · Biopolymers · doi:10.1002/bip.360221211 · Cited by 15,320
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The protein folding ‘speed limit’
Kubelka, J., Hofrichter, J., & Eaton, W. A. (2004) · Current Opinion in Structural Biology · doi:10.1016/j.sbi.2004.01.013 · Cited by 830
The complete folding pathway of a protein from nanoseconds to microseconds
Mayor, U., Guydosh, N. R., Johnson, C. M., Grossmann, J. G., Sato, S., Jas, G. S., Freund, S. M. V., Alonso, D. O. V., Daggett, V., & Fersht, A. R. (2003) · Nature · doi:10.1038/nature01428 · Cited by 458
Self-citationIs there a unifying mechanism for protein folding?
Daggett, V., & Fersht, A. R. (2003) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(02)00012-9 · Cited by 454
Self-citationFolding a protein in a computer: An atomic description of the folding/unfolding of protein A
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Metadata
- Authors
- Mayor, U., Johnson, C. M., Daggett, V., & Fersht, A. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2000-11-21
- Volume
- 97
- Issue
- 25
- Pages
- 13518-13522
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2088780328
- Cited by
- 316
- References
- 1
- FWCI
- 7.21
- Type
- journal article
- Language
- en
- Open access
- bronze
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