The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
A vertebrate fatty acid desaturase with Δ5 and Δ6 activities
Open accessAbstract
Δ5 and Δ6 fatty acid desaturases are critical enzymes in the pathways for the biosynthesis of the polyunsaturated fatty acids arachidonic, eicosapentaenoic, and docosahexaenoic acids. They are encoded by distinct genes in mammals and Caenorhabditis elegans . This paper describes a cDNA isolated from zebrafish ( Danio rerio ) with high similarity to mammalian Δ6 desaturase genes. The 1,590-bp sequence specifies a protein that, in common with other fatty acid desaturases, contains an N-terminal cytochrome b 5 domain and three histidine boxes, believed to be involved in catalysis. When the zebrafish cDNA was expressed in Saccharomyces cerevisiae it conferred the ability to convert linoleic acid (18:2 n -6) and α-linolenic acid (18:3 n -3) to their corresponding Δ6 desaturated products, 18:3 n -6 and 18:4 n -3. However, in addition it conferred on the yeast the ability to convert di-homo-γ-linoleic acid (20:3 n -6) and eicosatetraenoic acid (20:4 n -3) to arachidonic acid (20:4 n -6) and eicosapentaenoic acid (20:5 n -3), respectively, indicating that the zebrafish gene encodes an enzyme having both Δ5 and Δ6 desaturase activity. The zebrafish Δ5/Δ6 desaturase may represent a component of a prototypic vertebrate polyunsaturated fatty acids biosynthesis pathway.
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Self-citationPolyunsaturated fatty acids in the food chain in the United States
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Metabolism and Functions of Lipids and Fatty Acids in Teleost Fish
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Self-citationFish oil replacement in finfish nutrition
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Self-citationSTRUCTURE, FUNCTION, AND DIETARY REGULATION OF Δ6, Δ5, AND Δ9 DESATURASES
Nakamura, M. T., & Nara, T. Y. (2004) · Annual Review of Nutrition · doi:10.1146/annurev.nutr.24.121803.063211 · Cited by 1,073
Omega-3 long-chain polyunsaturated fatty acids and aquaculture in perspective
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Metadata
- Authors
- Hastings, N., Agaba, M., Tocher, D. R., Leaver, M. J., Dick, J. R., Sargent, J. R., & Teale, A. J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2001-11-27
- Volume
- 98
- Issue
- 25
- Pages
- 14304-14309
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1974172717
- Cited by
- 345
- References
- 35
- FWCI
- 8.24
- Type
- journal article
- Language
- en
- Open access
- green
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