The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Ferritin-Conjugated Plant Agglutinins as Specific Saccharide Stains for Electron Microscopy: Application to Saccharides Bound to Cell Membranes
Open accessAbstract
An electron microscopic stain for specific saccharides was prepared by the conjugation of ferritin to concanavalin A, a plant agglutinin that specifically binds to oligosaccharides containing terminal d -glucose, d -mannose, or sterically related sugar residues. A technique was developed to allow topological visualization of erythrocyte and other membranes by means of transmission electron microscopy, and the distribution of the binding sites for ferritin-concanavalin A on such membrane preparations was determined. The conjugate was found to bind specifically to the outer, but not the inner, surface of erythrocyte membranes. The number of conjugate molecules bound per unit area of the membrane was larger for rabbit than for human erythrocytes.
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Citations
Cites
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INTERACTION OF THE CARBOHYDRATE-BINDING PROTEIN CONCANAVALIN A WITH NORMAL AND TRANSFORMED CELLS
Inbar, M., & Sachs, L. (1969) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.63.4.1418 · Cited by 526
Identification of Hemagglutinin of Jack Bean with Concanavalin A
Sumner, J. B., & Howell, S. F. (1936) · Journal of Bacteriology · doi:10.1128/jb.32.2.227-237.1936 · Cited by 454
Red cell membrane: structure and function
Jamieson, G. A., & Greenwalt, T. J. (1969) · Lippincott eBooks · Cited by 378
Physical and chemical properties of a protein isolated from red cell membranes
Marchesi, S. L., Steers, E., Marchesi, V. T., & Tillack, T. W. (1970) · Biochemistry · doi:10.1021/bi00803a007 · Cited by 326
Structural Difference in Sites on the Surface Membrane of Normal and Transformed Cells
INBAR, M., & SACHS, L. (1969) · Nature · doi:10.1038/223710a0 · Cited by 311
Positive and negative colloidal iron as cell surface electron stains.
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SILVER IMPREGNATION OF ULTRATHIN SECTIONS FOR ELECTRON MICROSCOPY
Marinozzi, V. (1961) · The Journal of Cell Biology · doi:10.1083/jcb.9.1.121 · Cited by 179
Silver Impregnation Methods for Electron Microscopy
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Cited by
The Lectins: Carbohydrate-Binding Proteins of Plants and Animals
Goldstein, I. J., & Hayes, C. E. (1978) · Advances in Carbohydrate Chemistry and Biochemistry · doi:10.1016/s0065-2318(08)60220-6 · Cited by 1,621
Rothman, J. E., & Lenard, J. (1977) · Science · doi:10.1126/science.402030 · Cited by 986
Nicolson, G. L., Yanagimachi, R., & Yanagimachi, H. (1975) · The Journal of cell biology · doi:10.1083/jcb.66.2.263 · Cited by 484
Self-citationKim, K. J., Li, B., Houck, K., Winer, J., & Ferrara, N. (1992) · Growth Factors · doi:10.3109/08977199209023937 · Cited by 306
Properties of the Two Polypeptides of Sodium- and Potassium-dependent Adenosine Triphosphatase
Kyte, J. (1972) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)44573-0 · Cited by 265
Metadata
- Authors
- Nicolson, G. L., & Singer, S. J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1971-05
- Volume
- 68
- Issue
- 5
- Pages
- 942-945
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2035219404
- Cited by
- 274
- References
- 12
- FWCI
- 14.34
- Type
- journal article
- Language
- en
- Open access
- green
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- Nicolson, G. L., & Singer, S. J. (1971). Ferritin-Conjugated Plant Agglutinins as Specific Saccharide Stains for Electron Microscopy: Application to Saccharides Bound to Cell Membranes. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.68.5.942
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.