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SILVER IMPREGNATION OF ULTRATHIN SECTIONS FOR ELECTRON MICROSCOPY
Open accessAbstract
A new procedure is described for silver impregnation of thin sections for electron microscopy. Sections of various tissues, fixed in OsO4 and embedded in methacrylate, were treated with an ammoniacal silver solution, directly or after oxidation with periodic acid or hydrogen peroxide. After OsO4 fixation all cellular membranous systems exhibit a primary argentaffinity probably due to the reduction of ammoniacal silver solution by the reduced osmium bound to unsaturated lipids. Bleaching the sections with hydrogen peroxide removes the argentaffinity of protoplasmic structures. Treatment of the sections with periodic acid results in decreased argentaffinity of protoplasmic components while the argentaffinity of metaplasmic structures is greatly enhanced. The latter procedure appears particularly useful for enhancing the contrast of basement membranes.
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Cited by
Nicolson, G. L., & Singer, S. J. (1971) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.68.5.942 · Cited by 274
THE ORGANIC-INORGANIC RELATIONSHIP IN CALCIFIED MITOCHONDRIA
Bonucci, E., Derenzini, M., & Marinozzi, V. (1973) · The Journal of Cell Biology · doi:10.1083/jcb.59.1.185 · Cited by 83
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Metadata
- Authors
- Marinozzi, V.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1961-01-01
- Volume
- 9
- Issue
- 1
- Pages
- 121-133
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2041459680
- Cited by
- 179
- References
- 0
- FWCI
- 2.34
- Type
- journal article
- Language
- en
- Open access
- green
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- Marinozzi, V. (1961). SILVER IMPREGNATION OF ULTRATHIN SECTIONS FOR ELECTRON MICROSCOPY. The Journal of Cell Biology. https://doi.org/10.1083/jcb.9.1.121
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
