The DOI matches its Crossref record.
Construction of Biologically Functional Bacterial Plasmids In Vitro
Open accessAbstract
The construction of new plasmid DNA species by in vitro joining of restriction endonuclease-generated fragments of separate plasmids is described. Newly constructed plasmids that are inserted into Escherichia coli by transformation are shown to be biologically functional replicons that possess genetic properties and nucleotide base sequences from both of the parent DNA molecules. Functional plasmids can be obtained by reassociation of endonuclease-generated fragments of larger replicons, as well as by joining of plasmid DNA molecules of entirely different origins.
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Citations
Cited by
Design and self-assembly of two-dimensional DNA crystals
Winfree, E., Liu, F., Wenzler, L. A., & Seeman, N. C. (1998) · Nature · doi:10.1038/28998 · Cited by 2,857
A vision for the future of genomics research
Collins, F. S., Green, E. D., Guttmacher, A. E., Guyer, M. S., & on behalf of the US National Human Genome Research Institute (2003) · Nature · doi:10.1038/nature01626 · Cited by 1,752
Base editing: precision chemistry on the genome and transcriptome of living cells
Rees, H. A., & Liu, D. R. (2018) · Nature Reviews Genetics · doi:10.1038/s41576-018-0059-1 · Cited by 1,675
The sequence of sequencers: The history of sequencing DNA
Heather, J. M., & Chain, B. (2016) · Genomics · doi:10.1016/j.ygeno.2015.11.003 · Cited by 1,397
“The development of techniques such as polymerase chain reaction (PCR) [37,38] and recombinant DNA technologies[39,40]further aided the genomicsrevolution by pro-vidingmeans of generatingthe highconcentrationsof pure DNAspecies required for sequencing.”
MethodCounting absolute numbers of molecules using unique molecular identifiers
Kivioja, T., Vähärautio, A., Karlsson, K., Bonke, M., Enge, M., Linnarsson, S., & Taipale, J. (2012) · Nature Methods · doi:10.1038/nmeth.1778 · Cited by 1,010
Metadata
- Authors
- Cohen, S. N., Chang, A. C. Y., Boyer, H. W., & Helling, R. B.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1973-11
- Volume
- 70
- Issue
- 11
- Pages
- 3240-3244
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1995924881
- Cited by
- 1,802
- References
- 0
- FWCI
- 24.38
- Type
- journal article
- Language
- en
- Open access
- green
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- Cohen, S. N., Chang, A. C. Y., Boyer, H. W., & Helling, R. B. (1973). Construction of Biologically Functional Bacterial Plasmids In Vitro. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.70.11.3240
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
